Team:NTNU Trondheim/Demonstrate

• Team
  Team Members Collaborations
• Project
  Description Design Model Results Demonstrate
• Lab
  Lab Journal Protocols Measurement Safety
• Parts
  Parts Overview New Part
• InterLab
• Human Practices
  Integrated Human Practices Education and Engangement
• Acknowledgements
  Sponsors Attributions
• Awards

We were aiming to reduce the production of biofilm by silencing one significant gene for communication and signaling. To get a realistic result that would be of relevance in different industries we chose media conditions that would be similar to that in nature. By our Integrated Human Practices work we learned that different strains vary in the amount of biofilm produced, and that a strain that should be a good biofilm producer not necessarily proves to be in the laboratory. In addition we learned that the production is based on different parameters like pH, temperature and the presence of sugar. Therefore we tested 18 different media with different glucose concentrations and pH. In nature the resources often are limited, which gave us an indication on what conditions we should try to imitate. Based on the results we chose to do measurements with the minimal medium M63B1 with 0.4 and 0.8% glucose and pH 7.2. We also chose the E.Coli strain TG1, a strain that should produce biofilm in nature.