Team:CCU Taiwan/Notebook

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Notebook

NOTEBOOK

Notebook

February

    Molecular biology class
    E. coli genomic DNA preparation
    E. coli transformation

March

    Instrument operation

April

    Project brainstorming: Product Positioning, Monolignol proportion

May

    Culture selection: compare Yeast, E. coli, Acetobacter aceti
    Gene design

June

    Interlab experiment: Calibration 1, 2, 3

July

  7/3
    Start Interlab experiment: cell measurement
  7/5
    Yeast Extract–Peptone–Dextrose (YPD) formulation
  7/9
    Yeast (X33) culture
  7/13
    Fundraising briefing session
  7/16
    Communicate with NCKU (Interlab & project)
  7/18
    Communicate with BIT (project)

August

8/5~8/12

    Cell growth, sampling, and assay
    Digest pGAPZ A(X3)/ pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI
    Transformation pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18 (15µl) into 20µl ECOS™
    101 Competent Cells [DH5a]
    Ligation pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18
    Digest pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI

8/13~8/19

    Miniprep pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18
    Digest pGAPZ A(X3)/ pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI

8/20~8/26

  Gel extract
    Digestion – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)
    Ligation - pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18

8/27~9/2

    Digestion – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)
    Ligation – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)

    Transform
      1. pGAPZ A-1 + HIS4 + Px16
      2. pGAPZ A-1 + HIS4 + Px18
      3. pGAPZ A-1 + HIS4 + Lac1
    Digest pGAPZ A HIS4(X2) with AgeI, EcoRI

September

9/3~9/9

    PCR—pGAPZ A-1 + HIS4 + Px16, Px18, Lac1
    miniprep – pGAPZ A-1 + HIS4 + Px16, Px18
    Transform – pGAPZ A-1 + HIS4 + Lac1
    Digestion – pGAPZ A-1 + HIS4+lac1

9/12~9/14

    Colony PCR gel electrophoresis check - HIS4+Px16, HIS4+Lac1
    Culture HIS4+Px16, HIS4+Lac1
    Colony PCR gel electrophoresis check - HIS4+Px16, HIS4+Lac1
    Plasmid miniprep - HIS4+Lac1, HIS4+Px16

9/24~9/27

    Culture HIS4+Lac1 x3
    Prepare yeast competent cells (SMD1168)
    Traditional plasmid DNA extraction Day1
    Prepare YPD plates
    Prepare the amino acids of SD plates
    Traditional plasmid DNA extraction Day2
    Plasmids contain HIS4 cut with SalI
    Yeast transformation - Px16, Px18, Lac1 (plasmids contain HIS4)

October

10/8~10/11

    Prepare yeast competent cells (SMD1168)
    Colony PCR (HIS4+Px16)
    Safety (30℃, 50℃, 70℃)
    Yeast transformation - Px16, Lac1 (plasmids contain HIS4)