Team:TU-Eindhoven/Parts
Parts overview
To realize our designed parts, we gratefully made use of the IDT Synthesis offer, allowing easy and fast access to the desired DNA constructs. All parts are codon optimized for expression in E. coli, the workhorse of our project. For all our composite parts, the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed. We nominated BBa_K2812004, lysostaphin-thrombin linker-HlyA-His tag, as Best New Basic Part as we think that the realisation of secretable lysostaphin opens up a whole new area of projects and applications, since the issues and limitations associated with cell lysis are avoided. The T7-Lysostaphin-thrombin linker-HlyA-His part, BBa_K2812005, has been nominated for Best New Composite Part, as it allows direct secretion of lysostaphin from the iGEM shipping backbone. This enables future teams to have a head start in their project.
| Fave Part | Name | Type | Description | Designers | Length |
|---|---|---|---|---|---|
| ❤ | BBa_K2812000 | Basic | Carbohydrate-binding domain from MpIBP | Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls | 576 |
| BBa_K2812001 | Basic | Coding sequence for trunctated Lysostaphin | Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls | 738 | |
| BBa_K2812002 | Basic | pBAD-ara promoter - Arabinose inducible regulatory promoter | Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls | 71 | |
| BBa_K2812003 | Composite | Coding sequence for trunctated Lysostaphin regulated by T7-promoter | Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls | 776 | |
| ❤ | BBa_K2812004 | Basic | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls | 1458 |
| ❤ | BBa_K2812005 | Composite | Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter | Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls | 1496 |
| BBa_K2812006 | Basic | Coding sequence for Pyocin S5 with HlyA and His6-tag | Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls | 2217 | |
| BBa_K2812007 | Composite | Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter | Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls | 2288 |
