Throughout the years we meet all kinds of difficulties, and indeed, we could not overcome all of it. Here, we want to memorize all the E.coli with our highest respect. They contributed themselves in an unknown or known way to the known or unknown scientific problem. We recorded the les miserables of E.coli, with our sincere wishes their colleges in the future will not go through this again.
We did not have any data on this device, because the described RBS on that region was wayyyyyyyyyyyyyyy to far away from the start codon, so it is merely impossible to express the desired protein.