Protocols
E. coli transformation protocol
Please click on link to view the protocol for E. coli transformation.
Gel extraction protocol
Please click on link to view the protocol for gel extraction protocol.
Gibson assembly protocol
Please click on link to view the protocol for gibson assembly.
Agarose gel electrophoresis protocol
Please click on link to view the protocol for gel electrophoresis.
PCR protocol(Vazyme)
Please click on link to view the protocol for PCR(Vazyme).
PCR protocol(Trans)
Please click on link to view the protocol for PCR(Trans).
ABM assembly protocol
Please click on link to view the protocol for ABM assembly.
Protocol of preparing competent cells
Please click on link to view the protocol for preparing competent cells.
Protocol of purifying bacteria plasmids
Please click on link to view the protocol for purifying bacteria plasmids.
PCR extraction protocol
Please click on link to view the protocol for PCR extraction.
Characterization of CcaS-CcaR system
Please click on link to view the protocol for charactrizing CcaS-CcaR system.
Characterization of crRNA
Please click on link to view the charactrizing crRNA.
Characterization of split RNAP
Please click on link to view the protocol for charactrizing split RNAP.
Measurement of PHB production
Please click on link to view the protocol for measuring PHB production.
Nile red stain protocol
Please click on link to view the protocol for Nile red stain.