Difference between revisions of "Team:UNSW Australia/Lab"
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| − | <p> | + | <p>To express the various components of our scaffold for self-assembly experiments, our DNA constructs were cloned into plasmid vectors. Gibson assembly cloning methods were used to assemble the genes of interest with the vectors in an isothermal reaction. The products of the reaction were transformed into competent cells and colonies were screened for recombinant plasmids. 8 constructs were successfully cloned into corresponding pETDuet-1 and pRSFDuet-1 plasmids, while 6 constructs were cloned into pET-19b plasmids. </p> |
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Revision as of 11:28, 16 October 2018
Lab Overview
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Lab Sections
DNA Cloning
To express the various components of our scaffold for self-assembly experiments, our DNA constructs were cloned into plasmid vectors. Gibson assembly cloning methods were used to assemble the genes of interest with the vectors in an isothermal reaction. The products of the reaction were transformed into competent cells and colonies were screened for recombinant plasmids. 8 constructs were successfully cloned into corresponding pETDuet-1 and pRSFDuet-1 plasmids, while 6 constructs were cloned into pET-19b plasmids.
Protein Production
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Assembly
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FRET
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Enzyme Assays
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Plants
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