Bound Fraction &Unbound Fraction

By defining a simple equation where P is the promoter region and X is the ligand that can bind with P, namely a repressor in this case:

Intuitively, the factional saturation of the promoter is denoted by Ps , which is the fraction of occupied binding sites:

Modelling Equations

We provided team Tsinghua with IPTG assay with Ptac plasmid backbone in Tsinghua’s IPTG induction devices in their NEON system.Also we provided AHL for their Lux system.Tsinghua was generous to provide us some lab space and equipments to work on our projects. We worked close, both physically and intellectually, to create maximum impacts on our team projects.

Methods

This year, we provided SJTU-BioX-Shanghai with plasmids expressing acoustic reporter genes arg1 and arg2. They were applied to report the location of cancer foci combining with ultrasound in their project “E.Coli for Colon Health Observation”. We also shared protocols and equipments regarding non-invasive bacteria monitoring in our section of gas vesicles.

Results

Zeyu Tang from the team, RDFZ-China, kindly provided help on modeling for team GBC. To be specific, Zeyu gave them one document about previous work he has done on the fermentor model, which contains deterministic ODEs for predicting the productivity of engineered bacteria in the fermentor. In addition, Zeyu wrote another document which illustrates the deduction of Hill equation, helping them create their model simulation.

Knowing GreatBay was constructing a constitutive expressing GFP into the bacterial genome, we enquired for it and used it as a capacity monitor for characterizing the expression burden. Greatbay kindly gave it to us as a gift.

Discussion

Our team leader in 2017, Bowen Xiao, did a report on strategies preparing the iGEM competition; then the two teams shared resources and had a conversation on the biggest problems encounter during iGEM. Team BFSUICC-China in September met with us to discuss project updates and Jamboree preparation.