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Revision as of 17:13, 17 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

         
         

Demonstrate

        

Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve their gold medals!

Figure 1. Six 50 mL cultures of phaCB/Bktb in M9 media and 1% glucose were set up with three containing Pot ale and the other three containing water. Colonies were set up to shake at 37 oC overnight for 64 hours.



Figure 2. OD600 of cells grown in M9 media and 1% glucose with and without pot ale. Cells grown in pot ale and without pot ale similar growth curves.



Figure 3. Dry cell weight vs mass of plastic extracted of phaCB-Bktb cells grown in M9 media and 1% glucose with and without pot ale.



Figure 4. Melting temperature ranges of extracted plastic. Cultures grown on pot ale have consistant melting temperature ranges.