Difference between revisions of "Team:UNSW Australia/Parts"

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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<div class="header" id="parts-header">
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<img height="150px" width="150px" class="top-icon" src="https://static.igem.org/mediawiki/2018/c/c0/T--UNSW_Australia--Icon-parts.png">
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<img height="150px" width="150px" class="bottom-icon" src="https://static.igem.org/mediawiki/2018/f/fe/T--UNSW_Australia--Icon-parts-bw.png">
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<h1 class="shadow-text main-heading">Parts</h1>
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<p>All BioBrick parts were constructed from plasmids which were created by Gibson assembly of DNA gBlocks® synthesised by IDT. The relevant inserts were designed to contain the iGEM protein coding prefix and suffix and cloned into pETDuet™-1 or pRSFDuet™-1 plasmids using Gibson Assembly.</p>
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<p>These plasmids were digested and the inserts were ligated into linearised pSB1C3 plasmid backbone, to construct BioBrick parts using 3A assembly. All parts submitted to the registry were validated by sequence verification and agarose gel electrophoresis after digestion with EcoRI and PstI.</p>
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<p>Parts were also cloned into the pET19B expression vector for experimental characterisation. The protein coding parts were expressed in <em>Escherichia coli</em> T7 Express cells (NEB) and purified from cell lysates with Immobilised Metal Affinity Chromatography (IMAC) columns. The self-assembling functionality of our parts was further characterised by Size Exclusion Chromatography (SEC) and SDS-PAGE. From these experiments we were able to confirm the valid functionality of our parts, as they were experimentally expressed and purified, and confirmed to self-assemble as designed.</p>
  
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<table class="parts-table" width="100%">
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  <tr>
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    <th>Special Part</th>
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    <th>Part Number</th>
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    <th>Part Type</th>
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    <th>Description</th>
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    <th>Designer</th>
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<h3>Adding parts to the registry</h3>
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    <th>Length</th>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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    <td><a href="https://2018.igem.org/Team:UNSW_Australia/Basic_Part">Basic Part</a></td>
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<div class="button_link">
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    <td><a href="http://parts.igem.org/Part:BBa_K2710000">BBa_K2710000</a></td>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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    <td>Coding</td>
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ADD PARTS
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    <td>His-Alpha Prefoldin</td>
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</a>
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    <td>Brian Ee</td>
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    <td>456</td>
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  </tr>
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  <tr>
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    <td></td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2710001">BBa_K2710001</a></td>
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    <td>Coding</td>
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    <td>His-Beta Prefoldin</td>
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    <td>Brian Ee</td>
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    <td>396</td>
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  </tr>
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  <tr>
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    <td></td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2710002">BBa_K2710002</a></td>
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    <td>Coding</td>
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    <td>His-Alpha Prefoldin with SpyCatcher</td>
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    <td>Brian Ee</td>
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    <td>822</td>
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  </tr>
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  <tr>
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    <td></td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2710003">BBa_K2710003</a></td>
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    <td>Coding</td>
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    <td>His-Beta Prefoldin with SnoopCatcher</td>
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    <td>Brian Ee</td>
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    <td>759</td>
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  </tr>
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  <tr>
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    <td><a href="https://2018.igem.org/Team:UNSW_Australia/Improve">Improved Part</a></td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2710005">BBa_K2710005</a></td>
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    <td>Coding</td>
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    <td>His-IaaH with SpyTag</td>
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    <td>Brian Ee</td>
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    <td>1497</td>
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  </tr>
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</table>
 
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 UNSW_Australia</groupparts>
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Latest revision as of 18:09, 17 October 2018


Parts


All BioBrick parts were constructed from plasmids which were created by Gibson assembly of DNA gBlocks® synthesised by IDT. The relevant inserts were designed to contain the iGEM protein coding prefix and suffix and cloned into pETDuet™-1 or pRSFDuet™-1 plasmids using Gibson Assembly.

These plasmids were digested and the inserts were ligated into linearised pSB1C3 plasmid backbone, to construct BioBrick parts using 3A assembly. All parts submitted to the registry were validated by sequence verification and agarose gel electrophoresis after digestion with EcoRI and PstI.

Parts were also cloned into the pET19B expression vector for experimental characterisation. The protein coding parts were expressed in Escherichia coli T7 Express cells (NEB) and purified from cell lysates with Immobilised Metal Affinity Chromatography (IMAC) columns. The self-assembling functionality of our parts was further characterised by Size Exclusion Chromatography (SEC) and SDS-PAGE. From these experiments we were able to confirm the valid functionality of our parts, as they were experimentally expressed and purified, and confirmed to self-assemble as designed.

Special Part Part Number Part Type Description Designer Length
Basic Part BBa_K2710000 Coding His-Alpha Prefoldin Brian Ee 456
BBa_K2710001 Coding His-Beta Prefoldin Brian Ee 396
BBa_K2710002 Coding His-Alpha Prefoldin with SpyCatcher Brian Ee 822
BBa_K2710003 Coding His-Beta Prefoldin with SnoopCatcher Brian Ee 759
Improved Part BBa_K2710005 Coding His-IaaH with SpyTag Brian Ee 1497