Collaboration with NTHU_Taiwan
Team Collaboration
As a team composed of members from biology background, we gladly offer help and troubleshoot the problem on making constructs for team NTHU_Taiwan, being insufficient with members from biology department. Since August 3rd, we had several meetings
with 2018 iGEM team NTHU_Taiwan.
Earlier before the meeting, NTHU_Taiwan designed the gene of insert for their experiment. After getting the synthesized gene part from IDT and digesting both the inserts and the vectors, they proceeded to ligation, which failed after times
they tried.
We went through details from their design of the gene, cutting site and protocols. Later we concluded that the major reason for failing to make the constructs is due to low concentration of the inserts left following digestion and purification.
We re-designed primers for NTHU_Taiwan and suggested NEBuilder Assembly kit, so that they can avoid the loss of their inserts while digesting and purifying the DNA. The kit requires lower DNA input and can save time by avoiding PCR amplification
steps.
A few weeks later, we were informed that NTHU_Taiwan still failed to make the constructs. Therefore, we provided our lab for their experiments and kept an eye on them through out their experiments to make sure that there’s no man-made technical
false.
Gladly, after several meetup with us and our assistance in lab, team NTHU_Taiwan successfully made all three constructs they needed-- pSB1C3 - AHL snesor - Anti – STAR, pSB1C3 - pLac – STAR, pSB1C3 - pLux – GFP. It was very pleasant experience
to work with NTHU_Taiwan.
Since we urge to seek help in building wiki page, in return, NTHU_Taiwan also give us host a meeting with us teaching coding and giving us tips that help making wiki page easier for us. We took the wiki pages designed by previous teams
as examples to learn and practiced basic coding skills.
Validating construct used in our Biowatcher experiment is also involved in our collaboration.
Constructs from the left to right: pCAG-GBP, pTRE3G-LuxAB, pCMV-LuxCDEfrp and pTRE3G-mCherry. The size of the bands is corrected as expected.
