The optimisation potential of PHBV production is huge and as a result of our interaction with stakeholders, we developed a number of design requirements for our PHBV production process:

    - Use of optimised and innovative processes, such as the use of glucose as carbon source instead of feeding propionic acid into the system.

    - Use non-food crops and waste streams.

    - Innovative and more cost and environmental friendly processes for separation and purification.

    - Product design.

    - Introduction of sustainable end-of life scenarios such as re-use and recycling.

After incorporating all these design requirements in our final process, the final aim of the team to produce PHBV from waste or industrial by-products. Additionally, we wanted to reduce the impacts of the downstream processing, which is costly environmentally (see our LCA). To demonstrate this we used:

   - Bktb/phaCB operon

   - Whisky pot ale as raw material

   - Phasin and hemolysin secretion system

This section is intended to show how our parts and strategy were implemented and worked as a result of our stakeholders discussions and subsequent design.

Secreation

Growing on Whisky Pot Ale

We were able to grow our E. coli on media containing M9 salts, 1% glucose, and pot ale. Our negative control contained water instead of pot ale. Our cells grew similar in both types of media but grew lower than our model predicted. However, the pot ale did not negatively affect growth.

Next we wanted to show that our cells could produce plastic growing in pot ale. Cells were grown for 64 hours and then had their dry cell weight measured before extraction (Figure 3).