Difference between revisions of "Team:Edinburgh OG/Parts"

 
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         <th>Brief Description</th>
 
         <th>Brief Description</th>
 
         <th>Key Details</th>
 
         <th>Key Details</th>
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         <th>Length/th>
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         <th>Length</th>
 
         <th>Sequence information</th>
 
         <th>Sequence information</th>
 
       </tr>
 
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     <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
 
     <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
 
     <td>1459bp</td>
 
     <td>1459bp</td>
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     <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> Codon Optimised for E.coli</td>NCBI(AM260479.1). Codon Optimised for E.coli</td>
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     <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>. Sequence is codon optimised for <em>E.coli</em>.</td>
 
       </tr>
 
       </tr>
 
       <tr>   
 
       <tr>   
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      <td><a href="http://parts.igem.org/Part:BBa_K2739006">BBa_K2739006</a></td>
 
      <td><a href="http://parts.igem.org/Part:BBa_K2739006">BBa_K2739006</a></td>
 
      <td>proR-PhaR-proP-PhaP-HlyA</td>
 
      <td>proR-PhaR-proP-PhaP-HlyA</td>
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      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
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      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with the phasin native promoter </td>
 
      <td>1646bp</td>
 
      <td>1646bp</td>
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      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> Codon Optimised for E.coli</td>NCBI(AM260479.1) and BBa_K390501. Codon Optimised for E.coli</td>
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      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> and <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>. Part of PhaR is codon optimised for <em>E.coli</em>.</td>  
 
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  </tr>
 
  <tr>
 
  <tr>
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      <td>Phasin with HlyA signal peptide under the control of Lac promoter</td>
 
      <td>Phasin with HlyA signal peptide under the control of Lac promoter</td>
 
      <td>1139bp</td>
 
      <td>1139bp</td>
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      <td>This part is developed from BBa_K390501</td>
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      <td>This part is developed from: <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>.</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
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      <td>Co-expression of Bktb with PHA operon</td>
 
      <td>Co-expression of Bktb with PHA operon</td>
 
      <td>5462bp</td>
 
      <td>5462bp</td>
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      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a>. Codon Optimised for E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051</td>
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      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for <em>E.coli</em>.</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
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      <td>Bktb in replacing the phaA in PHA operon</td>
 
      <td>Bktb in replacing the phaA in PHA operon</td>
 
      <td>4252bp</td>
 
      <td>4252bp</td>
−
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a>. Codon Optimised for E.coli. The part of Hybrid promoter-PhaCB is developed from BBa_K1149051</td>
+
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for <em>E.coli</em>.</td>
 
  </tr>
 
  </tr>
 
     </tbody>
 
     </tbody>

Latest revision as of 01:22, 18 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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Parts

Registry Number Brief Description Key Details Length Sequence information
BBa_K2739005 proR-PhaR-Pha promoter-PhaP The phasin autoregulation system with its native promoter + phasin with its native promoter 1459bp Original Sequence obtained from: NCBI(AM260479.1). Sequence is codon optimised for E.coli.
BBa_K2739006 proR-PhaR-proP-PhaP-HlyA The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with the phasin native promoter 1646bp Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501. Part of PhaR is codon optimised for E.coli.
BBa_K2739007 LacP-RBS-PhaP-HlyA-Terminator Phasin with HlyA signal peptide under the control of Lac promoter 1139bp This part is developed from: BBa_K390501.
BBa_K2739009 Hybrid promoter-PhaCAB-Bktb Co-expression of Bktb with PHA operon 5462bp Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.
BBa_K2739010 Hybrid promoter-PhaCB-Bktb Bktb in replacing the phaA in PHA operon 4252bp Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.