Difference between revisions of "Team:XMU-China/Parts"

 
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     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/footer?action=raw&ctype=text/css">
 
     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/footer?action=raw&ctype=text/css">
 
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    <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/font?action=raw&ctype=text/css">
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     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/print?action=raw&ctype=text/css">
 
     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav_mobile?action=raw&ctype=text/css">
 
     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav_mobile?action=raw&ctype=text/css">
 
     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/material-scrolltop?action=raw&ctype=text/css">
 
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<body>
 
<body>
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    <div id="container">
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<header></header>
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<div id="container">
 
         <header>
 
         <header>
 
             <div class="wrapper cf">
 
             <div class="wrapper cf">
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
−
                                <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
 
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
 
                             </ul>
 
                             </ul>
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                             <ul>
 
                             <ul>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
−
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Microfluidic_Chips">Microfluidic Chips</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Fluorescence_Detection">Fluorescence Detection</a></li>
−
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Raspberry_Pi">Raspberry Pi</a></li>
−
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Application">Application</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Product Design</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                             <a href="#">Model</a>
 
                             <a href="#">Model</a>
 
                             <ul>
 
                             <ul>
−
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li>
+
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Model#Summary">Summary</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Thermodynamic_model">Thermodynamic Model</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Fluid_dynamics_model">Fluid dynamics Model</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Molecular_docking_model">Molecular Docking Model</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#The_dynamic_model">Derivation of Rate Equation</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
 
                         <li class="Human_Practice">
 
                         <li class="Human_Practice">
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                             <a href="#">Human Practice</a>
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                             <a href="#">Social Works</a>
 
                             <ul>
 
                             <ul>
−
                                 <li><a href="https://2018.igem.org/Te
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practice</a></li>
−
                                am:XMU-China/Human_Practices">Overview</a></li>
+
−
                                <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
+
−
                                <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
+
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
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                                 <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
 
                                 <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                         <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
 
                     </ul>
 
                     </ul>
 
                 </div>
 
                 </div>
 
                 <div id="Notebook">
 
                 <div id="Notebook">
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                     <div>
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                     <div class="nav-word">Notebook</div>
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                        <div class="nav-word">Notebook</a></div>
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−
                    </div>
+
 
                     <ul>
 
                     <ul>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
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                 </div>
 
                 </div>
 
                 <div id="Human_Practice">
 
                 <div id="Human_Practice">
−
                     <div class="nav-word">Human Practice</div>
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                     <div class="nav-word">Social Works</div>
 
                     <ul>
 
                     <ul>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Overview</a></li>
+
                         <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practice</a></li>
−
                        <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
+
−
                        <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
+
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
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                     <div class="nav-word">Model</div>
 
                     <div class="nav-word">Model</div>
 
                     <ul>
 
                     <ul>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li>
+
                         <li><a href="https://2018.igem.org/Team:XMU-China/Model">Summary</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Thermodynamic_model">Thermodynamic Model</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Fluid_dynamics_model">Fluid dynamics Model</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Molecular_docking_model">Molecular Docking Model</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#The_dynamic_model">Derivation of Rate Equation</a></li>
 
                     </ul>
 
                     </ul>
 
                 </div>
 
                 </div>
 
                 <div id="Hardwork">
 
                 <div id="Hardwork">
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                     <div class="nav-word">Hardwork</div>
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                     <div class="nav-word">Hardware</div>
 
                     <ul>
 
                     <ul>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li>
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                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Microfluidic_Chips">Microfluidic Chips</a></li>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li>
+
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Fluorescence_Detection">Fluorescence Detection</a></li>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li>
+
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Raspberry_Pi">Raspberry Pi</a></li>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li>
+
                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Application">Application</a></li>
−
                         <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li>
+
                         <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Product Design</a></li>
 
                     </ul>
 
                     </ul>
 
                 </div>
 
                 </div>
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                         <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
−
                        <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
 
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
 
                         <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
 
                     </ul>
 
                     </ul>
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         <nav class="Quick-navigation">
 
         <nav class="Quick-navigation">
 
             <div class="Quick-navigation_word">
 
             <div class="Quick-navigation_word">
−
                 <img  src="https://static.igem.org/mediawiki/2018/e/e6/T--XMU-China--right50.png">
+
                 <img  src="https://static.igem.org/mediawiki/2018/0/00/T--XMU-China--experiment_right10.png">
−
                 <a href="#Cell_Measurement" class="Quick-navigation-item">
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                 <a href="#Basic_Parts" class="Quick-navigation-item">
−
                     <img id="turn_img" src="https://static.igem.org/mediawiki/2018/5/50/T--XMU-China--right51.png">
+
                     <img id="turn_img" src="https://static.igem.org/mediawiki/2018/b/bb/T--XMU-China--experiment_right12.png">
−
                     <a href="#Cell_Measurement"id="Quick_A">Cell Measurement</a></a>
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                     <a href="#Basic_Parts"id="Quick_A">Basic Parts</a></a>
−
                 <a href="#Colony_Forming_Units" class="Quick-navigation-item" >
+
                 <a href="#Composite_Parts" class="Quick-navigation-item" >
−
                     <img id="turn_img" src="https://static.igem.org/mediawiki/2018/0/04/T--XMU-China--right52.png">
+
                     <img id="turn_img" src="https://static.igem.org/mediawiki/2018/5/50/T--XMU-China--experiment_right11.png">
−
                     <a href="#Colony_Forming_Units" id="Quick_B">Colony Forming Units</a></a>
+
                     <a href="#Composite_Parts" id="Quick_B">Composite Parts</a></a>
−
                <a href="#Experience_and_Discussion" class="Quick-navigation-item">
+
−
                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/d/d0/T--XMU-China--right53.png">
+
−
                    <a href="#Experience_and_Discussion" id="Quick_C">Experience and Discussion</a></a>
+
−
                <a href="#Provenance" class="Quick-navigation-item">
+
−
                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/1/11/T--XMU-China--right54.png">
+
−
                    <a href="#Provenance"id="Quick_D">Provenance</a></a>
+
−
                <a href="#Reference" class="Quick-navigation-item">
+
−
                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/b/ba/T--XMU-China--right55.png">
+
−
                    <a href="#Reference"id="Quick_F">Reference</a></a>
+
 
             </div>
 
             </div>
 
         </nav>
 
         </nav>
 
         <div class="main">
 
         <div class="main">
−
             <section id="Cell_Measurement" class="js-scroll-step">
+
            <section class="js-scroll-step">
 +
                <div class="headline"><br></div>
 +
                <p class="indent">This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click <span><a  class="click_here" href="https://2018.igem.org/Team:XMU-China/Design">here</a></span> to learn about our project, or browse the specific page on <span><a class="click_here" href="http://parts.igem.org/Main_Page">parts.igem.org</a></span> by clicking on the part numbers.</p>
 +
            </section>
 +
             <section id="Basic_Parts" class="js-scroll-step">
 
                 <div class="headline">
 
                 <div class="headline">
−
                     Parts
+
                     Basic Parts <a class="share" href="https://2018.igem.org/Team:XMU-China/Basic_Part"></a>
 
                 </div>
 
                 </div>
−
                 <p class="indent">This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click <span><a  class="click_here" href="https://2018.igem.org/Team:XMU-China/Design">here</a></span> to learn about our project, or browse the specific page on <span><a class="click_here" href="http://parts.igem.org/Main_Page">parts.igem.org</a></span> by clicking on the part numbers.</p>
+
                 <p class="indent">This year we have designed eleven basic parts mostly from TDP protein systems and Kai ABC systems. <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623008">BBa_K2623008</a></span> is our favorite basic part, which is the core part of the TDP circuit. This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.
−
                <h1>Basic Parts</h1>
+
−
                <p class="indent">This year we have designed eleven basic parts mostly from the Kai ABC systems. <a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a> is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
+
 
                 </p>
 
                 </p>
−
                 <table class="table">
+
               
 +
                 <div class="table_container">
 +
                <table class="table">
 
                     <tr>
 
                     <tr>
 
                         <th>Favorite</th>
 
                         <th>Favorite</th>
Line 240: Line 242:
 
                     <tr>
 
                     <tr>
 
                         <td>R</td>
 
                         <td>R</td>
−
                         <td><a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a></td>
+
                         <td><a href="http://parts.igem.org/Part:BBa_K2623008">BBa_K2623008</a></td>
−
                         <td>KaiC</td>
+
                         <td>CAHS</td>
−
                         <td>KaiC coding region</td>
+
                         <td>CAHS coding region</td>
 
                         <td>Coding</td>
 
                         <td>Coding</td>
−
                         <td>1560 bp</td>
+
                         <td>714 bp</td>
−
                         <td>Xinyi Ren</td>
+
                         <td>Weizhao Li</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
Line 264: Line 266:
 
                         <td>309 bp</td>
 
                         <td>309 bp</td>
 
                         <td>Yuhan Qin</td>
 
                         <td>Yuhan Qin</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a></td>
 +
                        <td>KaiC</td>
 +
                        <td>KaiC coding region</td>
 +
                        <td>Coding</td>
 +
                        <td>1560 bp</td>
 +
                        <td>Xinyi Ren</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
Line 286: Line 297:
 
                         <td></td>
 
                         <td></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623006">BBa_K2623006</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623006">BBa_K2623006</a></td>
−
                         <td>paA</td>
+
                         <td>RpaA</td>
−
                         <td>paA coding region</td>
+
                         <td>RpaA coding region</td>
 
                         <td>Coding</td>
 
                         <td>Coding</td>
 
                         <td>753 bp</td>
 
                         <td>753 bp</td>
Line 301: Line 312:
 
                         <td>Yingming Zhu</td>
 
                         <td>Yingming Zhu</td>
 
                     </tr>
 
                     </tr>
−
                     <tr>
+
                      
−
                        <td></td>
+
−
                        <td><a href="http://parts.igem.org/Part:BBa_K2623008">BBa_K2623008</a></td>
+
−
                        <td>CAHS</td>
+
−
                        <td>CAHS coding region</td>
+
−
                        <td>Coding</td>
+
−
                        <td>714 bp</td>
+
−
                        <td>Weizhao Li</td>
+
−
                    </tr>
+
 
                     <tr>
 
                     <tr>
 
                         <td></td>
 
                         <td></td>
Line 315: Line 318:
 
                         <td>pKaiBC</td>
 
                         <td>pKaiBC</td>
 
                         <td>pKaiBC promoter</td>
 
                         <td>pKaiBC promoter</td>
−
                         <td>egulatory</td>
+
                         <td>Regulatory</td>
 
                         <td>89 bp</td>
 
                         <td>89 bp</td>
 
                         <td>Gezhi Xiao</td>
 
                         <td>Gezhi Xiao</td>
Line 332: Line 335:
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td>NG7</td>
 
                         <td>NG7</td>
−
                         <td>siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026)</td>
+
                         <td>siRNA with C/D<sub>box</sub> RNA structure to bind with L7Ae (<a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a>)</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>1447 bp</td>
 
                         <td>1447 bp</td>
Line 338: Line 341:
 
                     </tr>
 
                     </tr>
 
                 </table>
 
                 </table>
−
                 <h1>Composite Parts</h1>
+
                 </div>
−
                 <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a>, which links <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.</p>
+
            </section>
−
                 <table class="table">
+
            <section id="Composite_Parts" class="js-scroll-step">
−
                     <tr>
+
                <div class="headline">
 +
                    Composite Parts <a class="share" href="https://2018.igem.org/Team:XMU-China/Composite_Part"></a>
 +
                </div>
 +
                 <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></span>, which links <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></span> and <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></span>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher.</p>
 +
               
 +
                 <div class="table_container">
 +
                <table class="table">
 +
                     <tr><span>
 
                         <th>Favorite</th>
 
                         <th>Favorite</th>
 
                         <th>Register Name</th>
 
                         <th>Register Name</th>
Line 352: Line 362:
 
                     <tr>
 
                     <tr>
 
                         <td>R</td>
 
                         <td>R</td>
−
                         <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
+
                         <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
−
                         <td>NG8-1</td>
+
                         <td>NG9-1</td>
−
                         <td>A composite part linked by BBa_K2623022 and BBa_K2623025</td>
+
                         <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>.</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>3410 bp</td>
 
                         <td>3410 bp</td>
−
                         <td>Niangui Cai</td>
+
                         <td>Yang Liang</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
Line 408: Line 418:
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
 
                         <td>NG1</td>
 
                         <td>NG1</td>
−
                         <td>BBa_K2623022 with GFP at its N-termini.</td>
+
                         <td><a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> with GFP at its N-termini</td>
 
                         <td>Reporter</td>
 
                         <td>Reporter</td>
 
                         <td>1657 bp</td>
 
                         <td>1657 bp</td>
Line 426: Line 436:
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
 
                         <td>NG3</td>
 
                         <td>NG3</td>
−
                         <td>Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini. </td>
+
                         <td>Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini </td>
 
                         <td>Reporter</td>
 
                         <td>Reporter</td>
 
                         <td>1645 bp</td>
 
                         <td>1645 bp</td>
Line 435: Line 445:
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
 
                         <td>NG4</td>
 
                         <td>NG4</td>
−
                         <td>A non-GFP form of the BBa_K2623023</td>
+
                         <td>A non-GFP form of the <a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>898 bp</td>
 
                         <td>898 bp</td>
Line 457: Line 467:
 
                         <td>2317 bp</td>
 
                         <td>2317 bp</td>
 
                         <td>Yunyun Hu</td>
 
                         <td>Yunyun Hu</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
 +
                        <td>NG8-1</td>
 +
                        <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></td>
 +
                        <td>Composite</td>
 +
                        <td>3410 bp</td>
 +
                        <td>Niangui Cai</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
Line 462: Line 481:
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623029</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623029</a></td>
 
                         <td>NG8-2</td>
 
                         <td>NG8-2</td>
−
                         <td>A composite part linked by BBa_K2623022 and BBa_K2623026.</td>
+
                         <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a>.</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>2698 bp</td>
 
                         <td>2698 bp</td>
 
                         <td>Ye Qiang</td>
 
                         <td>Ye Qiang</td>
 
                     </tr>
 
                     </tr>
−
                     <tr>
+
                      
−
                        <td></td>
+
−
                        <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
+
−
                        <td>NG9-1</td>
+
−
                        <td>A composite part linked by BBa_K2623024 and BBa_K2623025.</td>
+
−
                        <td>Composite</td>
+
−
                        <td>3410 bp</td>
+
−
                        <td>Yang Liang</td>
+
−
                    </tr>
+
 
                     <tr>
 
                     <tr>
 
                         <td></td>
 
                         <td></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
 
                         <td>NG9-2</td>
 
                         <td>NG9-2</td>
−
                         <td>A composite part linked by BBa_K2623024 and BBa_K2623026</td>
+
                         <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>2698 bp</td>
 
                         <td>2698 bp</td>
Line 489: Line 500:
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
 
                         <td>NG10</td>
 
                         <td>NG10</td>
−
                         <td>A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027</td>
+
                         <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a>, BBa_K2623026 and <a href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>4153 bp</td>
 
                         <td>4153 bp</td>
 
                         <td>Niangui Cai</td>
 
                         <td>Niangui Cai</td>
 
                     </tr>
 
                     </tr>
−
                   
 
 
                 </table>
 
                 </table>
 +
                </div>
 
             </section>
 
             </section>
 
         </div>
 
         </div>
Line 508: Line 519:
 
         <div class="footer">
 
         <div class="footer">
 
             <div class="footer_top">
 
             <div class="footer_top">
−
                <img src="https://static.igem.org/mediawiki/2018/3/3d/T--XMU-China--xmu_is_different.png">
+
                    <img src="https://static.igem.org/mediawiki/2018/3/3d/T--XMU-China--xmu_is_different.png">
−
                <ul>
+
                    <ul>
−
                    <li>Home</li>
+
                        <li><a href="https://2018.igem.org/Team:XMU-China">Home</a></a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Members">Members</a></li>
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Latest revision as of 01:57, 18 October 2018

Team:XMU-China/Parts - 2018.igem.org

Parts

This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click here to learn about our project, or browse the specific page on parts.igem.org by clicking on the part numbers.

Basic Parts

This year we have designed eleven basic parts mostly from TDP protein systems and Kai ABC systems. BBa_K2623008 is our favorite basic part, which is the core part of the TDP circuit. This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.

Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2623008 CAHS CAHS coding region Coding 714 bp Weizhao Li
BBa_K2623001 KaiA KaiA coding region Coding 852 bp Yi Lin
BBa_K2623002 KaiB KaiB coding region Coding 309 bp Yuhan Qin
BBa_K2623003 KaiC KaiC coding region Coding 1560 bp Xinyi Ren
BBa_K2623004 SasA SasA coding region Coding 1167 bp Yi Lin
BBa_K2623005 CikA CikA coding region Coding 2265 bp Yuhan Qin
BBa_K2623006 RpaA RpaA coding region Coding 753 bp Xinyi Ren
BBa_K2623007 SAHS SAHS coding region Coding 510 bp Yingming Zhu
BBa_K2623009 pKaiBC pKaiBC promoter Regulatory 89 bp Gezhi Xiao
BBa_K2623040 OmpA leader OmpA leader sequence Coding 72 bp Niangui Cai
BBa_K2623027 NG7 siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026) Composite 1447 bp Yunyun Hu
Composite Parts

We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623030, which links BBa_K2623024 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher.

Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2623030 NG9-1 A composite part linked by BBa_K2623024 and BBa_K2623025. Composite 3410 bp Yang Liang
BBa_K2623011 Kai 2 Phosphorylation pathsway builder of Kai Composite 4444 bp Gezhi Xiao
BBa_K2623013 Kai 1 3 Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC Composite 3978 bp Hongling Liu
BBa_K2623014 CAHS 1 Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) Composite 2891 bp Hongling Liu
BBa_K2623015 SAHS 1 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) Composite 1425 bp Jun Dai
BBa_K2623016 SAHS 2 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) Composite 1431 bp Yuanjun Shang
BBa_K2623021 NG1 BBa_K2623022 with GFP at its N-termini Reporter 1657 bp Niangui Cai
BBa_K2623022 NG2 Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini Composite 910 bp Qiupeng Wang
BBa_K2623023 NG3 Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini Reporter 1645 bp Junhong Chen
BBa_K2623024 NG4 A non-GFP form of the BBa_K2623023 Composite 898 bp Jiyang Zheng
BBa_K2623025 NG5 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. Reporter 2613 bp Ruofan Yang
BBa_K2623026 NG6 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. Composite 2317 bp Yunyun Hu
BBa_K2623028 NG8-1 A composite part linked by BBa_K2623022 and BBa_K2623025 Composite 3410 bp Niangui Cai
BBa_K2623029 NG8-2 A composite part linked by BBa_K2623022 and BBa_K2623026. Composite 2698 bp Ye Qiang
BBa_K2623031 NG9-2 A composite part linked by BBa_K2623024 and BBa_K2623026 Composite 2698 bp Junhong Chen
BBa_K2623032 NG10 A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 Composite 4153 bp Niangui Cai