Difference between revisions of "Team:UNSW Australia"

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{{UNSW_Australia}}
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{{Template:UNSW_Australia/Navbar}}
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{{Template:UNSW_Australia/Basics}}
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<html>
 
<html>
  
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<div id="home-content">
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                <div id="loading-image" class="shadow">
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                        <div id="background-image">
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                            <img src="https://static.igem.org/mediawiki/2018/c/cf/T--UNSW_Australia--Loading.png">
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                        </div>
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                        <div class="flex-center">
 +
                        <div id="header-text" width="50%">
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                                <h1 class="shadow-text main-heading">ASSEMBLASE</h1>
 +
                                <h2>Covalently <span class="red-text">Co-localising Enzymes</span> in a <span class="red-text">Modular</span> System</h2>
 +
                        </div>
 +
                        </div>
 +
                </div>
  
 +
<div id="gif-div">
 +
    <div id="unscaffolded" class="gif-box flex-center">
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        <div class="gif-img">
 +
            <img class="left-gif" src="https://static.igem.org/mediawiki/2018/d/d8/T--UNSW_Australia--Unscaffolded.gif">
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        </div>
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        <div class="gif-text">
 +
            <p class="big-text">Enzymes are ubiquitous to synthetic biology. We use them for everything, from the creation of therapeutics, to the development of novel bioremediation systems. Ensuring their efficiency is essential to the success of many synthetic biology projects. </p>
 +
<p class="big-text">The diffusion of reaction intermediates limits the efficiency of many biocatalytic pathways. The UNSW iGEM team has designed the <b>Assemblase self-assembling scaffold system</b> as the solution to this problem.</p>
 +
            <p class="big-text">The Assemblase scaffold specifically and covalently co-localises enzymes in a modular system. As a result, substrates can be channelled between enzymes at a much more efficient rate. This is due to the increased concentration of metabolic intermediates in the proximate surroundings of the enzymes. </p>
 +
<p class="big-text">Head over to our <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Description">description page</a> to find out how our system has been constructed.</p>
  
 +
        </div>
 +
    </div>
 +
    <div id="scaffolded" class="gif-box flex-center">
 +
        <div class="gif-text">
 +
            <p class="big-text">Our Assemblase scaffold has a range of advantages that make it ideal for a variety of applications. This includes it being highly thermostable and chemically resistant permiting our scaffold to be used at high temperatures, allowing for increased kinetic energy in our system and therefore an increased rate of catalysis.</p>
  
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<div class="column full_size" >
+
<p class="big-text">The chosen attachment system also affords our scaffold modularity. This is desirable as it means the scaffold can be easily adapted for use in a range of pathways important in industry, bioremediation, and pharmaceutical synthesis. </p>
−
<h1> Welcome to iGEM 2018! </h1>
+
<p class="big-text">Head over to our <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Design">design page</a> to find out how we chose the best components for our system.</p>
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
        </div>
 +
        <div class="gif-img">
 +
            <img class="right-gif" src="https://static.igem.org/mediawiki/2018/5/5f/T--UNSW_Australia--Scaffolded.gif">
 +
        </div>
 +
    </div>
 +
    <div id="unscaffolded" class="gif-box flex-center">
 +
        <div class="gif-img">
 +
            <img class="left-gif" src="https://static.igem.org/mediawiki/2018/5/53/T--UNSW_Australia--TeamD-01.png">
 +
        </div>
 +
        <div class="gif-text">
 +
            <p class="big-text">Over the past few months we have been busy <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Lab/Cloning">cloning DNA</a>, expressing and <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Lab/Protein">purifying some really cool proteins</a>, and attaching proteins together through self-assembly and with the Spy/Snoop Catcher/Tag system.</p>
 +
<p class="big-text">We also got to perform some really awesome <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Lab/Assays">enzyme assays</a> and <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Lab/FRET">FRET</a> experiments, alongside modelling our <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Model/EKD">enzyme kinetics</a> with some mathematical magic.</p>
 +
<p class="big-text">On top of this, we executed <a target="_blank" class="red-link" href="https://2018.igem.org/Team:UNSW_Australia/Model/MD">molecular dynamics</a> analysis, and grew our <a class="red-link" target="_blank" href="https://2018.igem.org/Team:UNSW_Australia/Lab/Plants">very own plants</a> on agar plates in the lab. We had a great year and would absolutely love to share it with you. So, have a look around, and explore all things UNSW iGEM!
 +
                </p>
 +
        </div>
 +
    </div>
 +
</div>
  
  
−
<img src="http://placehold.it/1080x320/c4baba/e4dede">
+
<div id="links">
−
+
<h2 class="text-center">Explore Our Project</h2>
 +
<br/>
 +
<div class="flex-center">
 +
<div id=description-icon>
 +
<a href="https://2018.igem.org/Team:UNSW_Australia/Description"><img class='link-icon' src=https://static.igem.org/mediawiki/2018/e/ea/T--UNSW_Australia--Description_Icon.png></a>
 +
<p class="text-center big-text">Description</p>
 +
</div>
 +
</div>
 +
<div class="flex-center">
 +
<div id=lab-icon>
 +
  <a href="https://2018.igem.org/Team:UNSW_Australia/Lab"><img class='link-icon' src=https://static.igem.org/mediawiki/2018/8/8d/T--UNSW_Australia--Icon-lab.png></a>
 +
  <p class="text-center big-text">Lab</p>
 +
</div>
 +
<div id=hp-icon>
 +
<a href="https://2018.igem.org/Team:UNSW_Australia/Human_Practices"><img class='link-icon' src="https://static.igem.org/mediawiki/2018/3/33/T--UNSW_Australia--Icon-hp.png"></a>
 +
<p class="text-center big-text">Human Practices</p>
 +
</div>
 +
</div>
 +
<div class="flex-center">
 +
<div id=design-icon>
 +
  <a href=https://2018.igem.org/Team:UNSW_Australia/Design><img class='link-icon' src=https://static.igem.org/mediawiki/2018/9/9b/T--UNSW_Australia--Icon-project.png></a>
 +
  <p class="text-center big-text">Design</p>
 +
</div>
 +
<div id=modelling-icon>
 +
  <a href="https://2018.igem.org/Team:UNSW_Australia/Model"><img class='link-icon' src=https://static.igem.org/mediawiki/2018/4/44/T--UNSW_Australia--Icon-modelling.png></a>
 +
  <p class="text-center big-text">Modelling</p>
 +
</div>
 +
</div>
 +
<div class="flex-center">
 +
<div id=team-icon>
 +
<a href="https://2018.igem.org/Team:UNSW_Australia/Team"><img class='link-icon' src=https://static.igem.org/mediawiki/2018/9/97/T--UNSW_Australia--Icon-people.png></a>
 +
  <p class="text-center big-text">Team</p>
 +
</div>
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</div>
 +
<br/>
  
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</div>  
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<div height="350px" width="100%">
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</div>
  
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<div class="flex-center" id="sponsor-footer">
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<img src=https://static.igem.org/mediawiki/2018/1/13/T--UNSW_Australia--sponsor-homepage.png>
 +
</div>
  
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<div class="column full_size" >
 
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<h3>Before you start</h3>
 
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<p> Please read the following pages:</p>
 
−
<ul>
 
−
<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
 
−
<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
−
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
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</ul>
 
 
</div>
 
</div>
  
  
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<div class="clear extra_space"></div>
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<style>
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<div class="line_divider"></div>
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#home-content {
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<div class="clear extra_space"></div>
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 +
}
  
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/* Main Banner Image */
  
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<div class="column full_size" >
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#home-content {
−
<h3> Styling your wiki </h3>
+
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
+
  
−
</div>
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}
  
 +
#sponsor-footer {
 +
  background-color: #333;
 +
  height: 150px;
 +
  width: 100%;
 +
  position: absolute;
 +
  bottom: 20px;
 +
  padding-top: 20px;
 +
}
  
 +
#footer p {
 +
  z-index: 3;
 +
}
  
 +
#sponsor-footer img {
 +
  z-index: 2;
 +
  height: 150px;
 +
}
  
−
<div class="clear extra_space"></div>
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#content {
 +
  padding-top: 0px;
 +
}
  
  
 +
@keyframes fade-in {
 +
  0%  {opacity: 0}
 +
  40% {opacity: 0}
 +
  100% {opacity: 1}
 +
}
  
−
<div class="column third_size" >
 
  
−
<h3> Uploading pictures and files </h3>
+
.circular-button {
−
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
+
  background-color: #dbdbdb;
 +
  position: relative;
 +
}
  
 +
#loading-image {
 +
        padding-top: 50px;
 +
animation-name: fade-in;
 +
        animation-duration: 3s;
 +
        margin: 0;
 +
        width: 100%;
 +
        height: 80vh;
 +
        padding-bottom: 30px;
 +
}
  
−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
#background-image {
 +
  height: 80vh;
 +
}
  
−
<div class="button_link">
+
.text-center {
−
<a href="https://2018.igem.org/Special:Upload">
+
  text-align: center !important;
−
UPLOAD FILES
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}
−
</a>
+
−
</div>
+
  
−
</div>
+
#background-image img {
 +
  width: 100%;
 +
  top: 50%;
 +
  transform: translateY(-50%);
 +
  position: relative;
 +
}
  
−
<div class="column third_size" >
+
#header-text {
−
<h3> Wiki template information </h3>
+
        width: 100vw;
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
        position: relative;
 +
        top: -50vh;
 +
        text-align: center !important;
 +
}
  
−
</div>
+
.main-heading {
 +
  font-size: 3.5rem !important;
 +
}
  
 +
#header-text h2 {
 +
  font-size: 2rem !important;
 +
  width: 50%;
 +
  margin: 0 auto;
 +
  line-height: 1.2;
 +
  padding: 0;
 +
}
  
 +
.red-text {
 +
  color: #9b3131;
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}
  
−
<div class="column third_size" >
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#abstract {
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<div class="highlight decoration_B_full">
+
  margin: 5px 10%;
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<h3> Editing your wiki </h3>
+
  padding-bottom: 50px;
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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}
−
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
  
−
<div class="button_link">
+
#abstract p, #abstract h2 {
−
<a href="https://2018.igem.org/wiki/index.php?title=Team:UNSW_Australia&action=edit">
+
  text-align: center !important;
−
EDIT PAGE
+
}
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</a>
+
−
</div>
+
  
 +
#gif-div {
 +
  width: 100vw;
 +
  background-color: #f0f0f0;
 +
  animation-name: fade-in;
 +
  animation-duration: 5s;
 +
}
  
−
</div>
+
.gif-img img {
−
</div>
+
    margin: 30px;
 +
    border: 1px solid #f0f0f0;
 +
    width: 35vw;
 +
    height: 30vw;
 +
    border-radius: 10px;
 +
}
  
 +
.gif-text {
 +
  margin: auto 10px;
 +
}
  
 +
.gif-box h2 {
 +
  text-align: center !important;
 +
}
  
 +
.gif-box p {
 +
  width: 50vw;
 +
}
  
 +
#description-icon {
 +
    margin: 0 auto -80px auto !important;
 +
}
  
−
<div class="clear extra_space"></div>
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#lab-icon {
−
<div class="line_divider"></div>
+
    margin: auto 100px 0 0 !important;
−
<div class="clear extra_space"></div>
+
}
  
 +
#hp-icon {
 +
    margin: auto 0 0 100px !important;
 +
}
  
 +
#design-icon {
 +
    margin: 0 100px auto 0 !important;
 +
}
  
−
<div class="column two_thirds_size" >
+
#modelling-icon {
−
<h3>Tips</h3>
+
    margin: 0 0 auto 100px !important;
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
}
−
<ul>
+
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>
+
−
</div>
+
  
 +
#team-icon {
 +
    margin: -80px auto 0 auto !important;
 +
}
  
−
<div class="column third_size">
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#links {
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<div class="highlight decoration_A_full">
+
  height: 850px;
−
<h3>Inspiration</h3>
+
}
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
 
−
<ul>
+
#links h2 {
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
  font-size: 2.5rem;
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
  padding-bottom: 30px;
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
}
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
 
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
.link-icon {
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
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−
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+
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−
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+
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−
</div>
+
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 +
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 +
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 +
 
 +
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 +
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 +
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 +
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 +
 
 +
.big-text {
 +
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 +
}
 +
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 +
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 +
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 +
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 +
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 +
 
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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Latest revision as of 02:24, 18 October 2018

ASSEMBLASE

Covalently Co-localising Enzymes in a Modular System

Enzymes are ubiquitous to synthetic biology. We use them for everything, from the creation of therapeutics, to the development of novel bioremediation systems. Ensuring their efficiency is essential to the success of many synthetic biology projects.

The diffusion of reaction intermediates limits the efficiency of many biocatalytic pathways. The UNSW iGEM team has designed the Assemblase self-assembling scaffold system as the solution to this problem.

The Assemblase scaffold specifically and covalently co-localises enzymes in a modular system. As a result, substrates can be channelled between enzymes at a much more efficient rate. This is due to the increased concentration of metabolic intermediates in the proximate surroundings of the enzymes.

Head over to our description page to find out how our system has been constructed.

Our Assemblase scaffold has a range of advantages that make it ideal for a variety of applications. This includes it being highly thermostable and chemically resistant permiting our scaffold to be used at high temperatures, allowing for increased kinetic energy in our system and therefore an increased rate of catalysis.

The chosen attachment system also affords our scaffold modularity. This is desirable as it means the scaffold can be easily adapted for use in a range of pathways important in industry, bioremediation, and pharmaceutical synthesis. 

Head over to our design page to find out how we chose the best components for our system.

Over the past few months we have been busy cloning DNA, expressing and purifying some really cool proteins, and attaching proteins together through self-assembly and with the Spy/Snoop Catcher/Tag system.

We also got to perform some really awesome enzyme assays and FRET experiments, alongside modelling our enzyme kinetics with some mathematical magic.

On top of this, we executed molecular dynamics analysis, and grew our very own plants on agar plates in the lab. We had a great year and would absolutely love to share it with you. So, have a look around, and explore all things UNSW iGEM!