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<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=0"> | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=0"> | ||
− | + | <!-- js --> | |
− | <!-- js | + | <script type="text/javascript" src="https://2018.igem.org/wiki/index.php?title=Template:GreatBay_China/jquery.js&action=raw&ctype=text/javascript"></script> |
− | <script src="https:// | + | <script type="text/javascript" src="https://2018.igem.org/wiki/index.php?title=Template:GreatBay_China/bootstrap.js&action=raw&ctype=text/javascript"></script> |
− | <script src="https:// | + | <!-- js popover --> |
− | + | ||
− | <!-- js | + | |
<script type="text/javascript"> | <script type="text/javascript"> | ||
$(function () { $("[data-toggle='popover']").popover(); }); | $(function () { $("[data-toggle='popover']").popover(); }); | ||
</script> | </script> | ||
+ | <!-- js menu --> | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
$(function(){ | $(function(){ | ||
− | $('.top-nav ul li span').hover(function(){ | + | $('.top-nav ul li span').hover(function(){$(this).addClass('hover');},function(){$(this).removeClass('hover');}); |
− | + | $('.top-nav ul li').mouseover(function(){$(this).children('div').stop().slideDown().parent().siblings().children('div').stop().slideUp();}); | |
− | },function(){ | + | $('.top-nav ul li span').mouseleave(function(){$(this).siblings('div').stop().slideUp()}); |
− | $(this).removeClass('hover'); | + | |
− | }); | + | |
− | $('.top-nav ul li').mouseover(function(){ | + | |
− | + | ||
− | }); | + | |
− | $('.top-nav ul li span').mouseleave(function(){ | + | |
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$('.top-nav ul li div').bind('mouseleave',function(){$(this).stop().slideUp();}); | $('.top-nav ul li div').bind('mouseleave',function(){$(this).stop().slideUp();}); | ||
− | }) | + | }) |
+ | |||
+ | </script> | ||
+ | <!-- js cathead --> | ||
+ | <script> | ||
+ | function changeface() | ||
+ | { | ||
+ | element=document.getElementById('menuhead') | ||
+ | if (element.style.display='none') | ||
+ | { | ||
+ | element.style.display='block'; | ||
+ | } | ||
+ | else | ||
+ | { | ||
+ | element.style.display='none'; | ||
+ | } | ||
+ | } | ||
</script> | </script> | ||
<!-- font --> | <!-- font --> | ||
− | < | + | <style> |
+ | /* latin */ | ||
+ | @font-face { | ||
+ | font-family: 'Delius'; | ||
+ | font-style: normal; | ||
+ | font-weight: 400; | ||
+ | src: local('Delius-Regular'), url(https://static.igem.org/mediawiki/2018/a/af/T--GreatBay_China--font.woff) format('woff'); | ||
+ | unicode-range: U+0000-00FF, U+0131, U+0152-0153, U+02BB-02BC, U+02C6, U+02DA, U+02DC, U+2000-206F, U+2074, U+20AC, U+2122, U+2191, U+2193, U+2212, U+2215, U+FEFF, U+FFFD; | ||
+ | } | ||
+ | </style> | ||
<!-- css main --> | <!-- css main --> | ||
<style> | <style> | ||
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.btn-primary[disabled].active, | .btn-primary[disabled].active, | ||
fieldset[disabled] .btn-primary.active { | fieldset[disabled] .btn-primary.active { | ||
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} | } | ||
.btn-primary .badge { | .btn-primary .badge { | ||
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.top-nav ul li div img{ | .top-nav ul li div img{ | ||
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− | + | position:relative; | |
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} | } | ||
#top{ | #top{ | ||
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left:-83px; | left:-83px; | ||
top:-30px; | top:-30px; | ||
+ | |||
+ | } | ||
+ | .top-nav ul li div .long{ | ||
+ | line-height:1.1em; | ||
+ | padding:8px; | ||
+ | font-size:0.85em; | ||
+ | |||
} | } | ||
/* link style */ | /* link style */ | ||
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/* submenu link style */ | /* submenu link style */ | ||
.top-nav ul li div span:hover{ | .top-nav ul li div span:hover{ | ||
− | background: | + | background:rgba(125, 63, 152, 0.4);; |
+ | |||
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.top-nav ul li div a:hover{ | .top-nav ul li div a:hover{ | ||
color:rgb(88,73,63); | color:rgb(88,73,63); | ||
− | background:rgb( | + | background:rgb(203,170,199); |
} | } | ||
/* special */ | /* special */ | ||
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.responsive .img-title{ | .responsive .img-title{ | ||
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− | + | color:rgb(88,73,63); | |
− | + | background-color:white; | |
− | + | padding-bottom:10px; | |
− | + | margin:0; | |
− | + | font-family:Delius; | |
− | + | text-align:center; | |
} | } | ||
.responsive div.img { | .responsive div.img { | ||
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− | left:- | + | left:-40px; |
− | top:- | + | top:-59px; |
z-index:10; | z-index:10; | ||
} | } | ||
#menuhead img{ | #menuhead img{ | ||
position:absolute; | position:absolute; | ||
− | width: | + | width: 490%; |
− | left:- | + | left:-160px; |
− | top:- | + | top:-42px; |
} | } | ||
</style> | </style> | ||
− | |||
<!-- <script type="application/x-javascript"> addEventListener("load", function() { setTimeout(hideURLbar, 0); }, false); function hideURLbar(){ window.scrollTo(0,1); } </script> --> | <!-- <script type="application/x-javascript"> addEventListener("load", function() { setTimeout(hideURLbar, 0); }, false); function hideURLbar(){ window.scrollTo(0,1); } </script> --> | ||
</head> | </head> | ||
<body> | <body> | ||
− | <div class="logo"><img src="https://static.igem.org/mediawiki/2018/1/14/T--GreatBay_China--logo1.jpg" /></div> | + | <div class="logo"><a><img src="https://static.igem.org/mediawiki/2018/1/14/T--GreatBay_China--logo1.jpg" /></a></div> |
+ | |||
+ | |||
+ | |||
<div class="container"> | <div class="container"> | ||
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<div class="top-nav"> | <div class="top-nav"> | ||
<ul> | <ul> | ||
− | <li | + | <li> |
<div class="res" target="_self" id="menuhead"> | <div class="res" target="_self" id="menuhead"> | ||
− | + | ||
<h5> Meow~</h5> | <h5> Meow~</h5> | ||
− | <img src="https://static.igem.org/mediawiki/2018/e/e4/T--GreatBay_China--menucathead.png" width="800px"> | + | <img onclick="changeface()" src="https://static.igem.org/mediawiki/2018/e/e4/T--GreatBay_China--menucathead.png" width="800px"></div> |
− | + | ||
</li> | </li> | ||
− | + | ||
<li> | <li> | ||
<a href="#"><span>Team</span></a> | <a href="#"><span>Team</span></a> | ||
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<a href="https://2018.igem.org/Team:GreatBay_China/Collaborations">Collaboration</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Collaborations">Collaboration</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Attributions">Attributions</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Attributions">Attributions</a> | ||
− | + | <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/> | |
− | + | </div> | |
</li> | </li> | ||
<li> | <li> | ||
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<a href="https://2018.igem.org/Team:GreatBay_China/Description">Description</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Description">Description</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Design">Design</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Design">Design</a> | ||
− | + | ||
− | <a href="https://2018.igem.org/Team:GreatBay_China/ | + | <a href="https://2018.igem.org/Team:GreatBay_China/Demonstrate">Results</a> |
− | + | ||
− | + | <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/> | |
− | + | ||
− | + | </div> | |
− | + | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href=" | + | <a href="#"><span>Parts</span></a> |
<div> | <div> | ||
+ | <a href="https://2018.igem.org/Team:GreatBay_China/Parts">Parts</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Basic_Part">Basic</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Basic_Part">Basic</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Composite_Part">Composite</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Composite_Part">Composite</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Part_Collection">Collection</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Part_Collection">Collection</a> | ||
− | + | <a href="https://2018.igem.org/Team:GreatBay_China/Improve" >Improve</a> | |
− | + | <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/> | |
− | + | ||
+ | </div> | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href=" | + | <a href="#"><span>HP</span></a> |
<div> | <div> | ||
− | + | <a href="https://2018.igem.org/Team:GreatBay_China/HP">Overview</a> | |
− | <a href="https://2018.igem.org/Team:GreatBay_China/Applied_Design">Product | + | <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Human_Practices">Integrated HP</a> |
− | <a href="https://2018.igem.org/Team:GreatBay_China/ | + | <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Applied_Design">Product Design</a> |
− | + | <a href="https://2018.igem.org/Team:GreatBay_China/Hardware">Hardware</a> | |
− | + | <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Public_Engagement">Public Engagement</a> | |
− | + | ||
+ | |||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/> | ||
+ | |||
+ | </div> | ||
</li> | </li> | ||
<li> | <li> | ||
<a href="#"><span>Lab</span></a> | <a href="#"><span>Lab</span></a> | ||
<div> | <div> | ||
+ | <a href="https://2018.igem.org/Team:GreatBay_China/Experiments">Experiments</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Notebook">Notebook</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Notebook">Notebook</a> | ||
<a href="https://2018.igem.org/Team:GreatBay_China/Interlab">Interlab</a> | <a href="https://2018.igem.org/Team:GreatBay_China/Interlab">Interlab</a> | ||
− | + | <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/> | |
− | + | ||
− | + | </div> | |
</li> | </li> | ||
<li> | <li> | ||
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<div class="row" style="margin:30px"> | <div class="row" style="margin:30px"> | ||
<span style="height:250px"></span> | <span style="height:250px"></span> | ||
− | <h1 style="color:rgb(88,73,63);font-size: | + | <h1 style="color:rgb(88,73,63);font-size:120px;font-family:Delius;margin-left:-80px;" class="text-center">mCATNIP</br></h1> |
<h1 style="color:rgb(88,73,63);font-size:45px;font-family:Delius;margin-left:-80px;" class="text-center">microbial Compartmentalization AssisTed </br>Nepetalactol Ingredient Production</br></h1> | <h1 style="color:rgb(88,73,63);font-size:45px;font-family:Delius;margin-left:-80px;" class="text-center">microbial Compartmentalization AssisTed </br>Nepetalactol Ingredient Production</br></h1> | ||
</br> | </br> | ||
</br> | </br> | ||
− | <p><video src="https://static.igem.org/mediawiki/2018/ | + | <p><video src="https://static.igem.org/mediawiki/2018/6/68/T--GreatBay_China--Documentary_PV.mp4" type="video/mov" height="auto" width="850" controls> |
</p> | </p> | ||
</div> | </div> | ||
+ | |||
<div class="row "> | <div class="row "> | ||
<span style="height:250px"></span> | <span style="height:250px"></span> | ||
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<button style="color:white;"type="button" class="btn btn-primary" title="Timshel" | <button style="color:white;"type="button" class="btn btn-primary" title="Timshel" | ||
data-container="body" data-toggle="popover" data-placement="top" | data-container="body" data-toggle="popover" data-placement="top" | ||
− | data-content="Meow!I am done! | + | data-content="Meow!I am done!"> |
Nepetalactone | Nepetalactone | ||
− | </button> is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between <i>E. coli</i> and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE) stabilized promoters to regulate the heterologous gene expression in <i>E. coli</i>. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education. | + | </button> is the active ingredient in <a href="https://en.wikipedia.org/wiki/Catnip" style="color:blue;">catnip</a>, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between <i>E. coli</i> and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (<a href="https://en.wikipedia.org/wiki/TAL_effector" style="color:blue;">TALE</a>)stabilized promoters to regulate the heterologous gene expression in <i>E. coli</i>. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education. Our presentation powerpoints can be download <a href="https://drive.google.com/open?id=1ya0kHbMCGpcZo6fegQNXOGzQMVNZzvG3" style="color:blue;">here</a>. |
</h4> | </h4> | ||
</div> | </div> | ||
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<div class="responsive "> | <div class="responsive "> | ||
<div class="img "> | <div class="img "> | ||
− | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/ | + | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Description"> |
<img src="https://static.igem.org/mediawiki/2018/2/24/T--GreatBay_China--HomologoProjectLogo1.png" alt="Project" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/2/24/T--GreatBay_China--HomologoProjectLogo1.png" alt="Project" width="300" height="200"> | ||
− | + | <div class="img-title">Project</div> | |
− | + | </a> | |
− | <div class="desc"> | + | <div class="desc"> If you want to know how to use synbio to create fantasies among cats, how to let two distintive microorganisms work together, how to achieve better stability and higher production, just click here and fill your curious brain with fun knowledge. </div> |
</div> | </div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2018/d/db/T--GreatBay_China--HomologoSafetyLogo1.png" alt="Safety" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/d/db/T--GreatBay_China--HomologoSafetyLogo1.png" alt="Safety" width="300" height="200"> | ||
<div class="img-title">Safety</div> | <div class="img-title">Safety</div> | ||
− | + | </a> | |
<div class="desc"> Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page. | <div class="desc"> Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page. | ||
<br> </div> | <br> </div> | ||
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<img src="https://static.igem.org/mediawiki/2018/9/9e/T--GreatBay_China--HomologoPartssLogo1.png" alt="Parts" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/9/9e/T--GreatBay_China--HomologoPartssLogo1.png" alt="Parts" width="300" height="200"> | ||
<div class="img-title">Parts</div> | <div class="img-title">Parts</div> | ||
− | + | </a> | |
− | <div class="desc"> We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand | + | <div class="desc"> We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand new collection of transcription-activator-like effector stabilised promoter.<br></div> |
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="responsive"> | <div class="responsive"> | ||
<div class="img"> | <div class="img"> | ||
− | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/ | + | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Notebook"> |
<img src="https://static.igem.org/mediawiki/2018/5/5c/T--GreatBay_China--HomologoLabLogo1.png" alt="Lab" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/5/5c/T--GreatBay_China--HomologoLabLogo1.png" alt="Lab" width="300" height="200"> | ||
<div class="img-title">Lab</div> | <div class="img-title">Lab</div> | ||
− | + | </a> | |
<div class="desc"> See how we have been working in the lab since May! We managed to achieve high yield of geraniol and successful co-culture in the end.</div> | <div class="desc"> See how we have been working in the lab since May! We managed to achieve high yield of geraniol and successful co-culture in the end.</div> | ||
</div> | </div> | ||
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<div class="responsive"> | <div class="responsive"> | ||
<div class="img "> | <div class="img "> | ||
− | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/ | + | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/HP"> |
<img src="https://static.igem.org/mediawiki/2018/d/d8/T--GreatBay_China--HomologoPartsLogo1.png" alt="HP" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/d/d8/T--GreatBay_China--HomologoPartsLogo1.png" alt="HP" width="300" height="200"> | ||
− | + | <div class="img-title">HP</div> | |
− | + | </a> | |
<div class="desc"> Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories. | <div class="desc"> Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories. | ||
<br> </div> | <br> </div> | ||
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<a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Model"> | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Model"> | ||
<img src="https://static.igem.org/mediawiki/2018/4/43/T--GreatBay_China--HomologoModelLogo1.png" alt="Model" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/4/43/T--GreatBay_China--HomologoModelLogo1.png" alt="Model" width="300" height="200"> | ||
− | + | <div class="img-title">Model</div> | |
− | + | </a> | |
<div class="desc"> Modeling tools aid in the rational design and make the engineered system predictable. Visit this page to take a closer look. | <div class="desc"> Modeling tools aid in the rational design and make the engineered system predictable. Visit this page to take a closer look. | ||
<br></div> | <br></div> | ||
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<a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Team"> | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Team"> | ||
<img src="https://static.igem.org/mediawiki/2018/f/f8/T--GreatBay_China--HomologoTeamLogo1.png" alt="Team" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/f/f8/T--GreatBay_China--HomologoTeamLogo1.png" alt="Team" width="300" height="200"> | ||
− | + | <div class="img-title">Team</div> | |
− | + | </a> | |
<div class="desc">GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here. | <div class="desc">GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here. | ||
</div> | </div> | ||
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<a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Judging"> | <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Judging"> | ||
<img src="https://static.igem.org/mediawiki/2018/0/00/T--GreatBay_China--HomologoJudgingLogo1.png" alt="Judging" width="300" height="200"> | <img src="https://static.igem.org/mediawiki/2018/0/00/T--GreatBay_China--HomologoJudgingLogo1.png" alt="Judging" width="300" height="200"> | ||
− | + | <div class="img-title">Judging</div> | |
− | + | </a> | |
<div class="desc"> This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards. | <div class="desc"> This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards. | ||
<br></div> | <br></div> | ||
</div> | </div> | ||
+ | |||
</div> | </div> | ||
− | < | + | <div class="row" style="padding-left:50px"> |
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
+ | |||
+ | |||
+ | <div class="row" style="padding-left:-50px" > | ||
+ | <div id="myCarousel" class="carousel slide" style=" | ||
+ | margin:50px; width:825px;padding-left: 50px;"> | ||
+ | <ol class="carousel-indicators"> | ||
+ | <li data-target="#myCarousel" data-slide-to="0" class="active"></li> | ||
+ | <li data-target="#myCarousel" data-slide-to="1"></li> | ||
+ | <li data-target="#myCarousel" data-slide-to="2"></li> | ||
+ | <li data-target="#myCarousel" data-slide-to="3"></li> | ||
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Latest revision as of 08:43, 7 December 2018
mCATNIP
microbial Compartmentalization AssisTed Nepetalactol Ingredient Production
Abstract
is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between E. coli and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE)stabilized promoters to regulate the heterologous gene expression in E. coli. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education. Our presentation powerpoints can be download here.
Project
If you want to know how to use synbio to create fantasies among cats, how to let two distintive microorganisms work together, how to achieve better stability and higher production, just click here and fill your curious brain with fun knowledge.
Safety
Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page.
Parts
We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand new collection of transcription-activator-like effector stabilised promoter.
Lab
See how we have been working in the lab since May! We managed to achieve high yield of geraniol and successful co-culture in the end.
HP
Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories.
Model
Modeling tools aid in the rational design and make the engineered system predictable. Visit this page to take a closer look.
Team
GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here.
Judging
This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards.