Difference between revisions of "Team:GreatBay China"

 
(31 intermediate revisions by 3 users not shown)
Line 9: Line 9:
 
   ================================================== -->
 
   ================================================== -->
 
<meta charset="utf-8">
 
<meta charset="utf-8">
−
  <!--[if lt IE 9]>
+
−
    <script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
+
−
  <![endif]-->
+
 
   <!-- Mobile Specific Metas
 
   <!-- Mobile Specific Metas
 
   ================================================== -->
 
   ================================================== -->
Line 48: Line 46:
 
</script>
 
</script>
 
<!-- font -->
 
<!-- font -->
−
<link rel="stylesheet" type="text/css" href="http://fonts.googleapis.com/css?family=Delius" />
+
<style>
 +
/* latin */
 +
@font-face {
 +
  font-family: 'Delius';
 +
  font-style: normal;
 +
  font-weight: 400;
 +
  src: local('Delius-Regular'), url(https://static.igem.org/mediawiki/2018/a/af/T--GreatBay_China--font.woff) format('woff');
 +
  unicode-range: U+0000-00FF, U+0131, U+0152-0153, U+02BB-02BC, U+02C6, U+02DA, U+02DC, U+2000-206F, U+2074, U+20AC, U+2122, U+2191, U+2193, U+2212, U+2215, U+FEFF, U+FFFD;
 +
}
 +
</style>
 
<!-- css main -->
 
<!-- css main -->
 
<style>
 
<style>
Line 6,994: Line 7,001:
  
 
   <div class="logo"><a><img src="https://static.igem.org/mediawiki/2018/1/14/T--GreatBay_China--logo1.jpg" /></a></div>
 
   <div class="logo"><a><img src="https://static.igem.org/mediawiki/2018/1/14/T--GreatBay_China--logo1.jpg" /></a></div>
 +
 +
 +
  
 
   <div class="container">  
 
   <div class="container">  
Line 7,023: Line 7,033:
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Design">Design</a>  
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Design">Design</a>  
 
                    
 
                    
−
                 <a href="https://2018.igem.org/Team:GreatBay_China/Results" >Results</a>  
+
                 <a href="https://2018.igem.org/Team:GreatBay_China/Demonstrate">Results</a>  
−
                 <a href="https://2018.igem.org/Team:GreatBay_China/Demonstrate">Demonstrate</a>
+
                  
−
                <a href="https://2018.igem.org/Team:GreatBay_China/Improve" >Improve</a>
+
 
           <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/>
 
           <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/>
 
          
 
          
Line 7,031: Line 7,040:
 
           </li>
 
           </li>
 
           <li>
 
           <li>
−
             <a href="https://2018.igem.org/Team:GreatBay_China/Parts"><span>Parts</span></a>  
+
             <a href="#"><span>Parts</span></a>  
 
               <div>
 
               <div>
 +
                  <a href="https://2018.igem.org/Team:GreatBay_China/Parts">Parts</a>
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Basic_Part">Basic</a>  
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Basic_Part">Basic</a>  
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Composite_Part">Composite</a>   
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Composite_Part">Composite</a>   
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Part_Collection">Collection</a>  
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Part_Collection">Collection</a>  
 +
                <a href="https://2018.igem.org/Team:GreatBay_China/Improve" >Improve</a>
 
           <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/>
 
           <img src="https://static.igem.org/mediawiki/2018/2/2e/T--GreatBay_China--AlexisWrite.jpgleaf.png"/>
 
          
 
          
Line 7,042: Line 7,053:
 
           </li>
 
           </li>
 
           <li>
 
           <li>
−
             <a href="https://2018.igem.org/Team:GreatBay_China/HP"><span>HP</span></a>
+
             <a href="#"><span>HP</span></a>
 
               <div>  
 
               <div>  
−
                 <a class="long" href="https://2018.igem.org/Team:GreatBay_China/HP/Human_Practices">Integrated HP</a>
+
                  <a href="https://2018.igem.org/Team:GreatBay_China/HP">Overview</a>
 +
                 <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Human_Practices">Integrated HP</a>
 
                 <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Applied_Design">Product Design</a>  
 
                 <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Applied_Design">Product Design</a>  
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Hardware">Hardware</a>  
 
                 <a href="https://2018.igem.org/Team:GreatBay_China/Hardware">Hardware</a>  
−
                 <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Engagement">Public Engagement</a>  
+
                 <a class="long" href="https://2018.igem.org/Team:GreatBay_China/Public_Engagement">Public Engagement</a>  
 
                  
 
                  
 
                  
 
                  
Line 7,155: Line 7,167:
 
         </br>
 
         </br>
 
          
 
          
−
         <p><video src="https://static.igem.org/mediawiki/2018/e/e4/T--GreatBay_China--video1.mov" type="video/mov" height="auto" width="850" controls>
+
         <p><video src="https://static.igem.org/mediawiki/2018/6/68/T--GreatBay_China--Documentary_PV.mp4" type="video/mov" height="auto" width="850" controls>
 
         </p>   
 
         </p>   
 
       </div>
 
       </div>
 +
 
       <div class="row ">
 
       <div class="row ">
 
         <span style="height:250px"></span>
 
         <span style="height:250px"></span>
Line 7,165: Line 7,178:
 
               <button style="color:white;"type="button" class="btn btn-primary" title="Timshel"   
 
               <button style="color:white;"type="button" class="btn btn-primary" title="Timshel"   
 
       data-container="body" data-toggle="popover" data-placement="top"  
 
       data-container="body" data-toggle="popover" data-placement="top"  
−
       data-content="Meow!I am done! I am finally done, by all means.">
+
       data-content="Meow!I am done!">
 
     Nepetalactone
 
     Nepetalactone
−
   </button> is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between <i>E. coli</i> and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE) stabilized promoters to regulate the heterologous gene expression in <i>E. coli</i>. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.
+
   </button> is the active ingredient in <a href="https://en.wikipedia.org/wiki/Catnip" style="color:blue;">catnip</a>, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between <i>E. coli</i> and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (<a href="https://en.wikipedia.org/wiki/TAL_effector" style="color:blue;">TALE</a>)stabilized promoters to regulate the heterologous gene expression in <i>E. coli</i>. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education. Our presentation powerpoints can be download <a href="https://drive.google.com/open?id=1ya0kHbMCGpcZo6fegQNXOGzQMVNZzvG3" style="color:blue;">here</a>.
 
           </h4>  
 
           </h4>  
 
         </div>
 
         </div>
Line 7,175: Line 7,188:
 
<div class="responsive ">
 
<div class="responsive ">
 
   <div class="img ">
 
   <div class="img ">
−
     <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Project">
+
     <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Description">
 
       <img src="https://static.igem.org/mediawiki/2018/2/24/T--GreatBay_China--HomologoProjectLogo1.png" alt="Project" width="300" height="200">
 
       <img src="https://static.igem.org/mediawiki/2018/2/24/T--GreatBay_China--HomologoProjectLogo1.png" alt="Project" width="300" height="200">
 
   <div class="img-title">Project</div>
 
   <div class="img-title">Project</div>
 
   </a>
 
   </a>
−
     <div class="desc">Metabolic engineering can help solve social issues! We envision to apply nepetalactone in stray cats rescue by employing VSD, making the use of synthetic biology readily comprehensible to the public. </div>
+
     <div class="desc"> If you want to know how to use synbio to create fantasies among cats, how to let two distintive microorganisms work together, how to achieve better stability and higher production, just click here and fill your curious brain with fun knowledge. </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 7,200: Line 7,213:
 
     <div class="img-title">Parts</div>
 
     <div class="img-title">Parts</div>
 
   </a>
 
   </a>
−
     <div class="desc">  We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand  
+
     <div class="desc">  We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand new collection of transcription-activator-like effector stabilised promoter.<br></div>
−
        <br></div>
+
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 7,219: Line 7,231:
 
<div class="responsive">
 
<div class="responsive">
 
   <div class="img ">
 
   <div class="img ">
−
     <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/Human_Practices">
+
     <a target="_blank" href="https://2018.igem.org/Team:GreatBay_China/HP">
 
       <img src="https://static.igem.org/mediawiki/2018/d/d8/T--GreatBay_China--HomologoPartsLogo1.png" alt="HP" width="300" height="200">
 
       <img src="https://static.igem.org/mediawiki/2018/d/d8/T--GreatBay_China--HomologoPartsLogo1.png" alt="HP" width="300" height="200">
 
     <div class="img-title">HP</div>
 
     <div class="img-title">HP</div>
Line 7,259: Line 7,271:
 
           <br></div>
 
           <br></div>
 
   </div>
 
   </div>
 +
 
</div>
 
</div>
−
</div>
+
  <div class="row" style="padding-left:50px">
−
<br><br><br><br>
+
−
      </div>
+
−
 
+
 
   </div>
 
   </div>
  
 +
 +
 +
  <div class="row" style="padding-left:-50px" >
 +
  <div id="myCarousel" class="carousel slide" style="
 +
  margin:50px; width:825px;padding-left: 50px;">
 +
    <ol class="carousel-indicators">
 +
        <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
 +
        <li data-target="#myCarousel" data-slide-to="1"></li>
 +
        <li data-target="#myCarousel" data-slide-to="2"></li>
 +
<li data-target="#myCarousel" data-slide-to="3"></li>
 +
<li data-target="#myCarousel" data-slide-to="4"></li>
 +
<li data-target="#myCarousel" data-slide-to="5"></li>
 +
    </ol> 
 +
    <div class="carousel-inner">
 +
        <div class="item active">
 +
            <img width="750" src="https://static.igem.org/mediawiki/2018/a/a6/T--GreatBay_China--p1.jpg" alt="First slide">
 +
        </div>
 +
        <div class="item">
 +
            <img width="750" src="https://static.igem.org/mediawiki/2018/9/99/T--GreatBay_China--p2.jpg" alt="Second slide">
 +
        </div>
 +
        <div class="item">
 +
            <img  width="750" src="https://static.igem.org/mediawiki/2018/f/fd/T--GreatBay_China--p3.jpg" alt="Third slide">
 +
        </div>
 +
<div class="item">
 +
            <img  width="750" src="https://static.igem.org/mediawiki/2018/e/ed/T--GreatBay_China--p4.jpg" alt="Fourth slide">
 +
        </div>
 +
<div class="item">
 +
            <img  width="750" src="https://static.igem.org/mediawiki/2018/b/bb/T--GreatBay_China--photo1.png" alt="Fifth slide">
 +
        </div>
 +
    </div>
 +
    <a class="carousel-control left" href="#myCarousel"
 +
      data-slide="prev"> <span _ngcontent-c3="" aria-hidden="true" class="glyphicon glyphicon-chevron-right"></span></a>
 +
    <a class="carousel-control right" href="#myCarousel"
 +
      data-slide="next">&rsaquo;</a>
 +
</div>
 
</div>
 
</div>
  
−
 
+
</div>
 +
<br><br><br><br>
 +
      </div>
 +
 
  
  

Latest revision as of 08:43, 7 December 2018

GBC-Home


mCATNIP2018@outlook.com
@Igem2018C

mCATNIP

microbial Compartmentalization AssisTed
Nepetalactol Ingredient Production



Abstract

is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between E. coli and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE)stabilized promoters to regulate the heterologous gene expression in E. coli. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education. Our presentation powerpoints can be download here.

Project
Project
If you want to know how to use synbio to create fantasies among cats, how to let two distintive microorganisms work together, how to achieve better stability and higher production, just click here and fill your curious brain with fun knowledge.
Safety
Safety
Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page.
Parts
Parts
We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand new collection of transcription-activator-like effector stabilised promoter.
Lab
Lab
See how we have been working in the lab since May! We managed to achieve high yield of geraniol and successful co-culture in the end.
HP
HP
Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories.
Model
Model
Modeling tools aid in the rational design and make the engineered system predictable. Visit this page to take a closer look.
Team
Team
GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here.
Judging
Judging
This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards.