Difference between revisions of "Team:Edinburgh OG"

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{{Edinburgh_OG}}
 
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                                <span>Antibiotic revolution: a molecular toolkit to re-sensitise </span><br>
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                                    <b class="is-visible">Methycillin resistant S.aureus (MRSA)</b>
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                                    <b>Vancomycin resistant S.aureus (VRSA)</b>
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                                    <b>Klebsiella pneumoniae</b>
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                                    <b>Pseudomonas aeruginosa</b>
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                                    <b>Enterococcus faecalis/faecium</b>
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                                    <b>Acinetobacter baumannii</b>
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                                    Modular molecular toolkit for re-sensitisation of antibiotic-resistant pathogens using CRISPR delivered by a two-phage system.
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                                </h2>
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<a class="btn-lines dark light wow fadeInUp animated smooth-scroll btn btn-default btn-green" data-wow-delay=".9s" href="#works" data-section="#about">Read more</a>
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                                ABOUT PhagED
 
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                                </h2>
 
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The threat posed by antibiotic resistant bacteria is a pressing issue which must be addressed. It is difficult and expensive to develop new antibiotics, so our project is designed to make currently available antibiotics useful again. Our aim is to create
 
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a toolkit to re-sensitise pathogens to antibiotics using CRISPR and a two-phage system, based on work by <b style="font-weight: bold;">Yosef et al. (2015, doi:10.1073/pnas.1500107112)</b>. An engineered lysogenic phage will transfer a CRISPR
 
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system to its host bacterium, designed to cleave resistance genes and also confer protection from an engineered lytic phage. When this lytic phage is added to the population, it kills any bacteria that have not been successfully re-sensitised. We
 
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chose to target genes found in the highly-resistant ESKAPE pathogens, and worked with 4 different phages - P1, lambda, T4 and T7. Our system was modelled in silico and tested empirically on a specially designed E. coli testing platform.
 
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Figure: Yosef et al., 2015
 
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<h1 class="title wow fadeInDown" data-wow-delay=".3s">TAKE A CLOSER LOOK</h1>
 
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Description">Details</a>
 
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                                <a href="https://2017.igem.org/Team:Edinburgh_OG/Description">
 
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                                    Project
 
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                                </a>
 
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Get to know PhagED better.
 
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Parts">Details</a>
 
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                                    Parts
 
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                                </a>
 
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Blocks of DNA that we've used.
 
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                                    Safety
 
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                                </a>
 
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Safety always comes first.
 
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/HP/Silver">Details</a>
 
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                                    Human Practices
 
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                                </a>
 
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How does PhagED fit into the context of our lives?
 
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<h1> Edinburgh OG </h1>
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<h2> About </h2>
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<p> The University of Edinburgh Overgraduate iGEM team this year is looking at making bioplastics. We want to look at a more innovative source for useful biodegradable plastics. Our plastic of choice is called poly(hydroxybutyrate-co-hydroxyvalerate) or PHBV. We are looking at using by-product from whisky distilleries, namely pot ale, to produce this plastic. PHBV holds a lot of promise because of its physical properties and can fit into the current plastic market and by using bio-mass, waste, or secondary carbon sources we are looking to decouple plastic production from petroleum. PHBV can also be biodegraded unlike most currently used plastics reducing the amount of devastating waste thrown into the environment. To create this plastic, we are trying to introduce enzymes into Escherichia coli to facilitate this production of PHBV efficiently.   </p>
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<h3 class="team_name">Filippo Abbondanza</h3>
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<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
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<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
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<h3 class="team_name">Erin Corbett</h3>
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<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
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<p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform, and engineering the lytic T7 phage.</p>
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<h3 class="team_name">Yunqi He</h3>
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<p class="team_designation">MSc Biochemistry</p>
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<p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
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<img src="https://static.igem.org/mediawiki/2017/3/35/T--Edinburgh_OG--ti.jpg" class="team-pic" alt="">
 
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<h3 class="team_name">Ti He</h3>
 
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<p class="team_designation">MSc Biotechnology</p>
 
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<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
 
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<a href="https://www.facebook.com/profile.php?id=100012772305809" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
 
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<h3 class="team_name">Lydia Mapstone</h3>
 
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<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
 
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<p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform and engineering the lytic T4 phage using BRED.</p>
 
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<h3 class="team_name">Yuri Matsueda</h3>
 
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<p class="team_designation">MSc Biotechnology</p>
 
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<p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
 
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<h3 class="team_name">Anton Puzorjov</h3>
 
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<p class="team_designation">MSc Bioinformatics</p>
 
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<p class="team_text">Building a model of bacteria-phage interactions in two-step re-sensitisation combining both lysogenic and lytic phages.</p>
 
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<p>The Edinburgh_OG team members developed the iGEM project as part of our <strong>Masters dissertations</strong> at the University of Edinburgh. Due to academic requirements, the iGEM project, PhagED, was divided into individual sub-projects to allow
 
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the production of unique dissertations. Each of us completed a dissertation based on the work we did for iGEM, and these dissertations are available upon request.</p>
 
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Revision as of 14:43, 29 September 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

Edinburgh OG

About

The University of Edinburgh Overgraduate iGEM team this year is looking at making bioplastics. We want to look at a more innovative source for useful biodegradable plastics. Our plastic of choice is called poly(hydroxybutyrate-co-hydroxyvalerate) or PHBV. We are looking at using by-product from whisky distilleries, namely pot ale, to produce this plastic. PHBV holds a lot of promise because of its physical properties and can fit into the current plastic market and by using bio-mass, waste, or secondary carbon sources we are looking to decouple plastic production from petroleum. PHBV can also be biodegraded unlike most currently used plastics reducing the amount of devastating waste thrown into the environment. To create this plastic, we are trying to introduce enzymes into Escherichia coli to facilitate this production of PHBV efficiently.