Difference between revisions of "Team:XMU-China/Parts"

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             <section id="Basic_Parts" class="js-scroll-step">
 
             <section id="Basic_Parts" class="js-scroll-step">
 
                 <div class="headline">
 
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                     Basic Parts
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                     Basic Parts <a class="share" href="https://2018.igem.org/Team:XMU-China/Basic_Part"></a>
 
                 </div>
 
                 </div>
 
                 <p class="indent">This year we have designed eleven basic parts mostly from the Kai ABC systems. <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a></span> is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
 
                 <p class="indent">This year we have designed eleven basic parts mostly from the Kai ABC systems. <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a></span> is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td>NG7</td>
 
                         <td>NG7</td>
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                         <td>siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026)</td>
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                         <td>siRNA with C/Dbox RNA structure to bind with L7Ae (<a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a>)</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>1447 bp</td>
 
                         <td>1447 bp</td>
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             <section id="Composite_Parts" class="js-scroll-step">
 
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                     Composite Parts
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                     Composite Parts<a class="share" href="https://2018.igem.org/Team:XMU-China/Composite_Part"></a>
 
                 </div>
 
                 </div>
 
                 <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></span>, which links <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a></span> and <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></span>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.</p>
 
                 <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></span>, which links <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a></span> and <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></span>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.</p>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
 
                         <td>NG8-1</td>
 
                         <td>NG8-1</td>
−
                         <td>A composite part linked by BBa_K2623022 and BBa_K2623025</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>3410 bp</td>
 
                         <td>3410 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
 
                         <td>NG1</td>
 
                         <td>NG1</td>
−
                         <td>BBa_K2623022 with GFP at its N-termini.</td>
+
                         <td><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> with GFP at its N-termini.</td>
 
                         <td>Reporter</td>
 
                         <td>Reporter</td>
 
                         <td>1657 bp</td>
 
                         <td>1657 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
 
                         <td>NG4</td>
 
                         <td>NG4</td>
−
                         <td>A non-GFP form of the BBa_K2623023</td>
+
                         <td>A non-GFP form of the <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>898 bp</td>
 
                         <td>898 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623029</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623029</a></td>
 
                         <td>NG8-2</td>
 
                         <td>NG8-2</td>
−
                         <td>A composite part linked by BBa_K2623022 and BBa_K2623026.</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a>.</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>2698 bp</td>
 
                         <td>2698 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
 
                         <td>NG9-1</td>
 
                         <td>NG9-1</td>
−
                         <td>A composite part linked by BBa_K2623024 and BBa_K2623025.</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>.</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>3410 bp</td>
 
                         <td>3410 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
 
                         <td>NG9-2</td>
 
                         <td>NG9-2</td>
−
                         <td>A composite part linked by BBa_K2623024 and BBa_K2623026</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>2698 bp</td>
 
                         <td>2698 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
 
                         <td>NG10</td>
 
                         <td>NG10</td>
−
                         <td>A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a>, BBa_K2623026 and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>4153 bp</td>
 
                         <td>4153 bp</td>

Revision as of 13:58, 12 October 2018

Team:XMU-China/Parts - 2018.igem.org

Parts

This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click here to learn about our project, or browse the specific page on parts.igem.org by clicking on the part numbers.

Basic Parts

This year we have designed eleven basic parts mostly from the Kai ABC systems. BBa_K2623003 is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.

Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2623003 KaiC KaiC coding region Coding 1560 bp Xinyi Ren
BBa_K2623001 KaiA KaiA coding region Coding 852 bp Yi Lin
BBa_K2623002 KaiB KaiB coding region Coding 309 bp Yuhan Qin
BBa_K2623004 SasA SasA coding region Coding 1167 bp Yi Lin
BBa_K2623005 CikA CikA coding region Coding 2265 bp Yuhan Qin
BBa_K2623006 paA paA coding region Coding 753 bp Xinyi Ren
BBa_K2623007 SAHS SAHS coding region Coding 510 bp Yingming Zhu
BBa_K2623008 CAHS CAHS coding region Coding 714 bp Weizhao Li
BBa_K2623009 pKaiBC pKaiBC promoter egulatory 89 bp Gezhi Xiao
BBa_K2623040 OmpA leader OmpA leader sequence Coding 72 bp Niangui Cai
BBa_K2623027 NG7 siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026) Composite 1447 bp Yunyun Hu
Composite Parts

We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623028, which links BBa_K2623022 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.

<
Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2623028 NG8-1 A composite part linked by BBa_K2623022 and BBa_K2623025 Composite 3410 bp Niangui Cai
BBa_K2623011 Kai 2 Phosphorylation pathsway builder of Kai Composite 4444 bp Gezhi Xiao
BBa_K2623013 Kai 1 3 Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC Composite 3978 bp Hongling Liu
BBa_K2623014 CAHS 1 Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) Composite 2891 bp Hongling Liu
BBa_K2623015 SAHS 1 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) Composite 1425 bp Jun Dai
BBa_K2623016 SAHS 2 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) Composite 1431 bp Yuanjun Shang
BBa_K2623021 NG1 BBa_K2623022 with GFP at its N-termini. Reporter 1657 bp Niangui Cai
BBa_K2623022 NG2 Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini Composite 910 bp Qiupeng Wang
BBa_K2623023 NG3 Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini. Reporter 1645 bp Junhong Chen
BBa_K2623024 NG4 A non-GFP form of the BBa_K2623023 Composite 898 bp Jiyang Zheng
BBa_K2623025 NG5 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. Reporter 2613 bp Ruofan Yang
BBa_K2623026 NG6 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. Composite 2317 bp Yunyun Hu
BBa_K2623029 NG8-2 A composite part linked by BBa_K2623022 and BBa_K2623026. Composite 2698 bp Ye Qiang
BBa_K2623030 NG9-1 A composite part linked by BBa_K2623024 and BBa_K2623025. Composite 3410 bp Yang Liang
BBa_K2623031 NG9-2 A composite part linked by BBa_K2623024 and BBa_K2623026 Composite 2698 bp Junhong Chen
BBa_K2623032 NG10 A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 Composite 4153 bp Niangui Cai
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