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Revision as of 14:24, 13 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens


 

 

 

 

Attributions

 

We are tremendously grateful to everyone who has kindly helped and guided us throughout our project. Your kindness is truly appreciated!

 

Team Valeris.ED 

  • Cheewin (Big) Kittikunapong : Team Leader, Crowdfunding, Integrated Human Practices Team, In silico modelling of project

  • Jackson DeKloe : Team Leader, Integrated Human Practices Team, InterLab study, Glucose toggle switch development project

  • Lo Lok In (Owen) : Jamboree trip coordinator, Integrated Human Practices Team, Public Engagement, Secretion system project

  • Ivonne Robledo : Integrated Human Practices Team, Collaborated with Westminster iGEM team, Life cycle assessment project

  • Nabila Akhyar : Logo Designer, Integrated Human Practices Team, Wiki Manager, Life cycle assessment project

  • Ming Zheng : Wiki team, bktB gene project

  • Siqi Wang : Wiki team, Integrated Human Practices Team, sucCD gene project

  • Craig Gilmour : Collaborated with Iowa team, SBM operon project

  • Qihui Lian : phaR gene-based autoregulation project

  • Peiyu Lu : Integrated Human Practices Team, PHA operon project

    •  

    Supervisors and Advisors

  • Dr Louise Horsfall : Primary Instructor, supervised us on project planning, integrated human practices and academic work, provided her lab after dissertation submission for our project

  • Holly Robertson-Dick : Secondary Instructor, supervised our iGEM work and advised us on iGEM requirements and fundraising

  • Dr Annegret Honsbein : helped the team with troubleshooting and provided advice on planning experiments

  • Dr Heather Barker : supervised our lab work and integrated human practices

  • Michael McDonald : supervised our lab work

  • Dr Elise Cachat : advised us on project planning and academic work

  • Dr David Clarke : advised us on project planning and technical support

    •  

    Project Support

  • Dr David Hills and Dr Elena Sugrue : looked after us in the lab

  • Dr Leonardo Rios Solis : advised us on simulation using SuperPro Designer

  • Dr Camilla Thomson : advised us on life cycle assessment using SimaPro

  • Dr Ramon Grima : guided us on dynamic modelling approach

  • Daniel Xiang, Stanford-Brown iGEM 2015 : provided dynamic approach model adapted in this project

  • Dr Martin Waterfall : helped us with the InterLab study

  • Edinburgh UG 2018 Team : collaborated with us in the InterLab study

  • Westminster 2018 Team : collaborated with us in the Life Cycle Assessment

  • Iowa UG 2018 Team : collaborated with us in the detection of propionyl Co-A by their biosensor

  • Prof Paul Michels : advised us on metabolic pathway

  • Dr Horsfall Lab : provided lab space and guidance

  • William Bothfeld, Northwestern University : provided us with LacI(knock out) MG1655 E. coli, pKDL071 plasmid, pKDL071-TaraF plasmid, and pKDL071-TaraF-phaECAB plasmid

  • Alan Taylor : provided us access to Gas Chromatography and Mass Spectrophotometry, and advised us on the methodology and testings

  • Alan Isbister, John Hopkins, and Kirsten Affleck : provided access to PCR machines and other equipment including flasks, shaker space, chemicals

  • Susan Forbes and Vicki Brown : provided access to shaker space and drying oven

  • Susan Rosser’s lab : provided access to chemicals and enzymes as well as shaker space

  • Dr Tessa Moses : helped with gas chromatography inquiry

  • Jessica Birt : advised on stoichiometric modelling and provided Benchling introduction

  • Christine Merrick and James Bryson : helped with the competent cells

  • Felipe Aguilera Millacura : helped with developing Sudan Black staining as an alternative strategy to confirm the production of PHA

  • Graham Nicholson and David Sneddon: helped us to buy chemicals and anything we ran out of

  • Margaret Martin and her team : helped to prepare medium and autoclaved flasks

  • Dr Jane Paget and Dr Hille Tekotte, Edinburgh Genome Foundry : helped with cloning design, plate reader and quantitative measurement of PHA production

  • Dr David Kelly : provided fluorescent microscopy for Nile red staining test

  • Ian Eggington and Miguel Cueva : helped with cloning and primers design

  • Prof Mark Blaxter and Edinburgh Genomics : contacted Edinburgh Genomics and helped us get 800 GBP of free sequencing

  • Radhian Ferrel Armansyah : Web designer (Wiki)

  • Everyone who had generously supported us on our crowdfunding page

  • Dr Lorrain Kerr, Commercial Relations Manager : provided information and contacts for Integrated Human Practices

  • MSc students in Tools of Synthetic Biology class 2017/2018 : provided valuable input that directed our project

  • Dr James Hallinan, Business Development Manager Synthetic Biology; Dr James Brown, Principal Scientist; and Dr Steve Thomas, Senior Consultant of Cambridge Consultant : provided information

  • Dr Adrian Higson, Director and Lead Consultant for Biobased Products of The National Non-Food Crops Centre (NNFCC) :

  • Morag Garden, Head of Sustainability and Innovation of Scotch Whisky Association :

  • Dr Guoqiang Chen, Tsinghua University and BLUEPHA :

  • Jin Yin, Technique Manager of BLUEPHA:

  • Huang Jianfeng, Synthetic Resin Sales Division Manager of China Sinopec Guangzhou Branch :

  • Paul Tan, Officer of China Sinopec Guangzhou Branch :

    • Liu Hui, Manager of Tianan Biologic Material Co. Ltd. :