Difference between revisions of "Team:Edinburgh OG/Parts"

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<p>-</p>
 
<p>-</p>
 
<p>-</p>
 
<p>-</p>
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<table width="100%"  cellspacing="0" height="60px">
 
<table width="100%"  cellspacing="0" height="60px">
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   <tr><td  bgColor="#FEE5AD"></td> <td colspan="3" width="975px" bgColor="#FEE5AD" align="center"><h1>Parts ! ! !</h2></td> <td  bgColor="#FEE5AD"></td> </tr>
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   <tr><td  bgColor="#FEE5AD"></td> <td colspan="3" width="975px" bgColor="#FEE5AD" align="center"><h1>Our Best Improved Part ! ! !</h2></td> <td  bgColor="#FEE5AD"></td> </tr>
 
</table>
 
</table>
−
 
  
 
<div class="column two_thirds_size" >
 
<div class="column two_thirds_size" >
 +
<p>We have selected this part as the best improved part in the project because, </p>
 +
<ul>
 +
<li> 1. </li>
 +
<li> 2. </li>
 +
<li> 3. </li>
 +
</ul>
 +
 +
 
<table class="table table-hover" style="margin-left:-40px;">
 
<table class="table table-hover" style="margin-left:-40px;">
 
     <thead>
 
     <thead>
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         <td>ProR-PhaR </td>
 
         <td>ProR-PhaR </td>
 
         <td>The phasin autoregulation system with native promoter</td>
 
         <td>The phasin autoregulation system with native promoter</td>
−
         <td>N/A</td>
+
         <td>658bp</td>
 
         <td>Original Sequence obtained from:  . Codon Optimised for E.coli</td>
 
         <td>Original Sequence obtained from:  . Codon Optimised for E.coli</td>
 
       </tr>
 
       </tr>
 +
    </tbody>
 +
  </table>
 +
  </div>
 +
 +
 +
<table width="100%"  cellspacing="0" height="60px">
 +
  <tr><td colspan="3" width="975px" align="center"><h1>Parts </h1></td>  </tr>
 +
</table>
 +
 +
 +
<div class="column two_thirds_size" >
 +
<table class="table table-hover" style="margin-left:-40px;">
 +
    <thead>
 +
      <tr>
 +
        <th>Registry Number</th>
 +
        <th>Brief Description</th>
 +
        <th>Key Details</th>
 +
        <th>Length/th>
 +
        <th>Sequence information</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 
       <tr>
 
       <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2739005">BBa_K2739005</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K2739005">BBa_K2739005</a></td>
−
     <td>proR-PhaR-proP promoter-PhaP</td>
+
     <td>proR-PhaR-Pha promoter-PhaP</td>
 
     <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
 
     <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
−
     <td>N/A</td>
+
     <td>1459bp</td>
 
     <td>Original Sequence obtained from:  . Codon Optimised for E.coli</td>
 
     <td>Original Sequence obtained from:  . Codon Optimised for E.coli</td>
 
       </tr>
 
       </tr>
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      <td>proR-PhaR-proP-PhaP-HlyA</td>
 
      <td>proR-PhaR-proP-PhaP-HlyA</td>
 
      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
 
      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
−
      <td>N/A</td>
+
      <td>1646bp</td>
 
      <td>Original Sequence obtained from:  . Codon Optimised for E.coli</td>
 
      <td>Original Sequence obtained from:  . Codon Optimised for E.coli</td>
 
  </tr>
 
  </tr>
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      <td>LacP-RBS-PhaP-HlyA-Terminator</td>
 
      <td>LacP-RBS-PhaP-HlyA-Terminator</td>
 
      <td>Phasin with HlyA signal peptide under the control of Lac promoter</td>
 
      <td>Phasin with HlyA signal peptide under the control of Lac promoter</td>
−
      <td>BBa_K390501</td>
+
      <td>1139bp</td>
−
      <td>N/A</td>
+
      <td>This part is developed from BBa_K390501</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
 
      <td><a href="http://parts.igem.org/Part:BBa_K2739009">BBa_K2739009</a></td>
 
      <td><a href="http://parts.igem.org/Part:BBa_K2739009">BBa_K2739009</a></td>
 
      <td>Hybrid promoter-PhaCAB-Bktb</td>
 
      <td>Hybrid promoter-PhaCAB-Bktb</td>
−
      <td>N/A</td>
+
      <td>Co-expression of Bktb with PHA operon</td>
−
      <td>N/A</td>
+
      <td>5462bp</td>
−
      <td>N/A</td>
+
      <td>The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
 
      <td><a href="http://parts.igem.org/Part:BBa_K2739010">BBa_K2739010</a></td>
 
      <td><a href="http://parts.igem.org/Part:BBa_K2739010">BBa_K2739010</a></td>
 
      <td>Hybrid promoter-PhaCB-Bktb</td>
 
      <td>Hybrid promoter-PhaCB-Bktb</td>
−
      <td>N/A</td>
+
      <td>Bktb in replacing the phaA in PHA operon</td>
−
      <td>N/A</td>
+
      <td>4252bp</td>
−
      <td>N/A</td>
+
      <td>The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCB is developed from BBa_K1149051</td>
 
  </tr>
 
  </tr>
 
     </tbody>
 
     </tbody>
 
   </table>
 
   </table>
 
   </div>
 
   </div>
 +
 +
  
  
  
 
</html>
 
</html>

Revision as of 23:51, 14 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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Our Best Improved Part ! ! !

We have selected this part as the best improved part in the project because,

  • 1.
  • 2.
  • 3.
Registry Number Brief Description Key Details Length/th> Sequence information
BBa_K2739000 ProR-PhaR The phasin autoregulation system with native promoter 658bp Original Sequence obtained from: . Codon Optimised for E.coli

Parts

Registry Number Brief Description Key Details Length/th> Sequence information
BBa_K2739005 proR-PhaR-Pha promoter-PhaP The phasin autoregulation system with its native promoter + phasin with its native promoter 1459bp Original Sequence obtained from: . Codon Optimised for E.coli
BBa_K2739006 proR-PhaR-proP-PhaP-HlyA The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter 1646bp Original Sequence obtained from: . Codon Optimised for E.coli
BBa_K2739007 LacP-RBS-PhaP-HlyA-Terminator Phasin with HlyA signal peptide under the control of Lac promoter 1139bp This part is developed from BBa_K390501
BBa_K2739009 Hybrid promoter-PhaCAB-Bktb Co-expression of Bktb with PHA operon 5462bp The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051
BBa_K2739010 Hybrid promoter-PhaCB-Bktb Bktb in replacing the phaA in PHA operon 4252bp The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCB is developed from BBa_K1149051