Difference between revisions of "Team:Edinburgh OG/Parts"

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<p>We have selected this part as the best improved part in the project because, </p>
 
<p>We have selected this part as the best improved part in the project because, </p>
 
<ul>
 
<ul>
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<li> 1. </li>
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<li> 1. This composite part has allow us to investigate A)  its relation in PHA production through PHA operon B) its function on PHA production with Phasin C) Assess the secretion function of Phasin-HlyA is compatible with phaR and if the improvement of phaR of PHA production is remained in this parts composition</li>
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<li> 2. </li>
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<li> 2. The function part of the sequence is more properly selected</li>
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<li> 3. </li>
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<li> 3. The sequence is codon optimised to E.coli</li>
 
</ul>
 
</ul>
  

Revision as of 23:58, 14 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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Our Best Improved Part ! ! !

We have selected this part as the best improved part in the project because,

  • 1. This composite part has allow us to investigate A) its relation in PHA production through PHA operon B) its function on PHA production with Phasin C) Assess the secretion function of Phasin-HlyA is compatible with phaR and if the improvement of phaR of PHA production is remained in this parts composition
  • 2. The function part of the sequence is more properly selected
  • 3. The sequence is codon optimised to E.coli
Registry Number Brief Description Key Details Length/th> Sequence information
BBa_K2739000 ProR-PhaR The phasin autoregulation system with native promoter 658bp Original Sequence obtained from: . Codon Optimised for E.coli

Parts

Registry Number Brief Description Key Details Length/th> Sequence information
BBa_K2739005 proR-PhaR-Pha promoter-PhaP The phasin autoregulation system with its native promoter + phasin with its native promoter 1459bp Original Sequence obtained from: . Codon Optimised for E.coli
BBa_K2739006 proR-PhaR-proP-PhaP-HlyA The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter 1646bp Original Sequence obtained from: . Codon Optimised for E.coli
BBa_K2739007 LacP-RBS-PhaP-HlyA-Terminator Phasin with HlyA signal peptide under the control of Lac promoter 1139bp This part is developed from BBa_K390501
BBa_K2739009 Hybrid promoter-PhaCAB-Bktb Co-expression of Bktb with PHA operon 5462bp The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051
BBa_K2739010 Hybrid promoter-PhaCB-Bktb Bktb in replacing the phaA in PHA operon 4252bp The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCB is developed from BBa_K1149051