Difference between revisions of "Team:Edinburgh OG/Parts"

Line 39: Line 39:
 
      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
 
      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
 
      <td>1646bp</td>
 
      <td>1646bp</td>
−
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> and <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>. Part of PhaR is codon optimised for E.coli</td>  
+
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> and <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>. Part of PhaR is codon optimised for E.coli.</td>  
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
Line 46: Line 46:
 
      <td>Phasin with HlyA signal peptide under the control of Lac promoter</td>
 
      <td>Phasin with HlyA signal peptide under the control of Lac promoter</td>
 
      <td>1139bp</td>
 
      <td>1139bp</td>
−
      <td>This part is developed from: <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a></td>
+
      <td>This part is developed from: <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>.</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
Line 53: Line 53:
 
      <td>Co-expression of Bktb with PHA operon</td>
 
      <td>Co-expression of Bktb with PHA operon</td>
 
      <td>5462bp</td>
 
      <td>5462bp</td>
−
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for E.coli</td>
+
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for E.coli.</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
Line 60: Line 60:
 
      <td>Bktb in replacing the phaA in PHA operon</td>
 
      <td>Bktb in replacing the phaA in PHA operon</td>
 
      <td>4252bp</td>
 
      <td>4252bp</td>
−
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and BBa_K1149051. Part of Bktb is codon optimised for E.coli</td>
+
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and BBa_K1149051. Part of Bktb is codon optimised for E.coli.</td>
 
  </tr>
 
  </tr>
 
     </tbody>
 
     </tbody>

Revision as of 18:48, 16 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

-

-

-

-

Parts

Registry Number Brief Description Key Details Length Sequence information
BBa_K2739005 proR-PhaR-Pha promoter-PhaP The phasin autoregulation system with its native promoter + phasin with its native promoter 1459bp Original Sequence obtained from: NCBI(AM260479.1). Sequence is codon optimised for E.coli.
BBa_K2739006 proR-PhaR-proP-PhaP-HlyA The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter 1646bp Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501. Part of PhaR is codon optimised for E.coli.
BBa_K2739007 LacP-RBS-PhaP-HlyA-Terminator Phasin with HlyA signal peptide under the control of Lac promoter 1139bp This part is developed from: BBa_K390501.
BBa_K2739009 Hybrid promoter-PhaCAB-Bktb Co-expression of Bktb with PHA operon 5462bp Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.
BBa_K2739010 Hybrid promoter-PhaCB-Bktb Bktb in replacing the phaA in PHA operon 4252bp Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.