Difference between revisions of "Team:ZJUT-China/InterLab"
| Line 864: | Line 864: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 1 </th> |
| − | <th></th> | + | <th>-6.05</th> |
| − | <th></th> | + | <th> 698.91 </th> |
| − | <th></th> | + | <th>76.22 </th> |
| − | <th></th> | + | <th>295.93 </th> |
| − | <th></th> | + | <th>5.40</th> |
| − | <th></th> | + | <th> 352.20 </th> |
| − | <th></th> | + | <th>5.61</th> |
| − | <th></th> | + | <th>29.13</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 2 </th> |
| − | <th></th> | + | <th>-4.46 </th> |
| − | <th></th> | + | <th>648.50</th> |
| − | <th></th> | + | <th> 66.80 </th> |
| − | <th></th> | + | <th>323.64 </th> |
| − | <th></th> | + | <th>4.37</th> |
| − | <th></th> | + | <th> 360.68</th> |
| − | <th></th> | + | <th> 7.11 </th> |
| − | <th></th> | + | <th>23.40</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 3</th> |
| − | <th></th> | + | <th> -9.93 </th> |
| − | <th></th> | + | <th>689.59</th> |
| − | <th></th> | + | <th> 68.91 </th> |
| − | <th></th> | + | <th>300.19</th> |
| − | <th></th> | + | <th> -2.06 </th> |
| − | <th></th> | + | <th>338.91 </th> |
| − | <th></th> | + | <th>0.60 </th> |
| − | <th></th> | + | <th>22.85</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 4 </th> |
| − | <th></th> | + | <th>-4.45 </th> |
| − | <th></th> | + | <th>699.73 </th> |
| − | <th></th> | + | <th>98.45 </th> |
| − | <th></th> | + | <th>265.60</th> |
| − | <th></th> | + | <th> 12.05 </th> |
| − | <th></th> | + | <th>401.30</th> |
| − | <th></th> | + | <th> 8.33</th> |
| − | <th></th> | + | <th> 19.84</th> |
</tr> | </tr> | ||
</table> | </table> | ||
<table> | <table> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Hour 6: </th> |
| − | <th></th> | + | <th>Neg. Control </th> |
| − | <th></th> | + | <th>Pos. Control </th> |
| − | <th></th> | + | <th>Device 1</th> |
| − | <th></th> | + | <th> Device 2</th> |
| − | <th></th> | + | <th> Device 3</th> |
| − | <th></th> | + | <th> Device 4 </th> |
| − | <th></th> | + | <th>Device 5</th> |
| − | <th></th> | + | <th> Device 6</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 1 </th> |
| − | <th></th> | + | <th>11.65 </th> |
| − | <th></th> | + | <th>1448.71 </th> |
| − | <th></th> | + | <th>276.87</th> |
| − | <th></th> | + | <th> 2137.68 </th> |
| − | <th></th> | + | <th>36.92 </th> |
| − | <th></th> | + | <th>884.06 </th> |
| − | <th></th> | + | <th>13.28 </th> |
| − | <th></th> | + | <th>235.19</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 2 </th> |
| − | <th></th> | + | <th>16.60 </th> |
| − | <th></th> | + | <th>1466.59 </th> |
| − | <th></th> | + | <th>270.31 </th> |
| − | <th></th> | + | <th>2201.58 </th> |
| − | <th></th> | + | <th>48.22 </th> |
| − | <th></th> | + | <th>876.80</th> |
| − | <th></th> | + | <th> 14.59 </th> |
| − | <th></th> | + | <th>212.86</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 3</th> |
| − | <th></th> | + | <th> 23.60 </th> |
| − | <th></th> | + | <th>1571.41 </th> |
| − | <th></th> | + | <th>268.76 </th> |
| − | <th></th> | + | <th>2047.59 </th> |
| − | <th></th> | + | <th>41.80 </th> |
| − | <th></th> | + | <th>849.69 </th> |
| − | <th></th> | + | <th>15.85 </th> |
| − | <th></th> | + | <th>219.80</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 4 </th> |
| − | <th></th> | + | <th>28.56 </th> |
| − | <th></th> | + | <th>1655.83 </th> |
| − | <th></th> | + | <th>278.97 </th> |
| − | <th></th> | + | <th>2112.89 </th> |
| − | <th></th> | + | <th>44.06 </th> |
| − | <th></th> | + | <th>916.85 </th> |
| − | <th></th> | + | <th>26.90 </th> |
| − | <th></th> | + | <th>213.65</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 1 </th> |
| − | <th></th> | + | <th>29.58 </th> |
| − | <th></th> | + | <th>1942.08 </th> |
| − | <th></th> | + | <th>233.55 </th> |
| − | <th></th> | + | <th>1969.35 </th> |
| − | <th></th> | + | <th>44.31 </th> |
| − | <th></th> | + | <th>1147.30 </th> |
| − | <th></th> | + | <th>29.80 </th> |
| − | <th></th> | + | <th>200.06</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 2 </th> |
| − | <th></th> | + | <th>18.48 </th> |
| − | <th></th> | + | <th>1896.07 </th> |
| − | <th></th> | + | <th>239.26 </th> |
| − | <th></th> | + | <th>2042.66 </th> |
| − | <th></th> | + | <th>40.51 </th> |
| − | <th></th> | + | <th>1076.68 </th> |
| − | <th></th> | + | <th>17.36</th> |
| − | <th></th> | + | <th> 195.85</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 3 </th> |
| − | <th></th> | + | <th>15.35 </th> |
| − | <th></th> | + | <th>1970.55 </th> |
| − | <th></th> | + | <th>221.82 </th> |
| − | <th></th> | + | <th>1795.61 </th> |
| − | <th></th> | + | <th>52.52 </th> |
| − | <th></th> | + | <th>1041.35 </th> |
| − | <th></th> | + | <th>26.93 </th> |
| − | <th></th> | + | <th>201.75</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 4 </th> |
| − | <th></th> | + | <th>19.68 </th> |
| − | <th></th> | + | <th>1933.63 </th> |
| − | <th></th> | + | <th>178.57 </th> |
| − | <th></th> | + | <th>1612.39 </th> |
| − | <th></th> | + | <th>41.45 </th> |
| − | <th></th> | + | <th>1115.62 </th> |
| − | <th></th> | + | <th>17.80 </th> |
| − | <th></th> | + | <th>189.56</th> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 1,013: | Line 1,013: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Hour 0:</th> |
| − | <th></th> | + | <th>Neg. Control</th> |
| − | <th></th> | + | <th>Pos. Control</th> |
| − | <th></th> | + | <th>Device 1</th> |
| − | <th></th> | + | <th>Device 2</th> |
| − | <th></th> | + | <th>Device 3</th> |
| − | <th></th> | + | <th>Device 4</th> |
| − | <th></th> | + | <th>Device 5</th> |
| − | <th></th> | + | <th>Device 6</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 1 </th> |
| − | <th></th> | + | <th>0.082 </th> |
| − | <th></th> | + | <th>0.040 </th> |
| − | <th></th> | + | <th>0.028 </th> |
| − | <th></th> | + | <th>0.058 </th> |
| − | <th></th> | + | <th>0.070 </th> |
| − | <th></th> | + | <th>0.027 </th> |
| − | <th></th> | + | <th>0.058 </th> |
| − | <th></th> | + | <th>0.038</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 2 </th> |
| − | <th></th> | + | <th>0.078 </th> |
| − | <th></th> | + | <th>0.041 </th> |
| − | <th></th> | + | <th>0.024 </th> |
| − | <th></th> | + | <th>0.058 </th> |
| − | <th></th> | + | <th>0.069 </th> |
| − | <th></th> | + | <th>0.026 </th> |
| − | <th></th> | + | <th>0.058 </th> |
| − | <th></th> | + | <th>0.040</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 3 </th> |
| − | <th></th> | + | <th>0.078 </th> |
| − | <th></th> | + | <th>0.044 </th> |
| − | <th></th> | + | <th>0.028 </th> |
| − | <th></th> | + | <th>0.066 </th> |
| − | <th></th> | + | <th>0.075 </th> |
| − | <th></th> | + | <th>0.027 </th> |
| − | <th></th> | + | <th>0.062</th> |
| − | <th></th> | + | <th> 0.042</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 4 </th> |
| − | <th></th> | + | <th>0.075 </th> |
| − | <th></th> | + | <th>0.040 </th> |
| − | <th></th> | + | <th>0.027 </th> |
| − | <th></th> | + | <th>0.063 </th> |
| − | <th></th> | + | <th>0.077 </th> |
| − | <th></th> | + | <th>0.027 </th> |
| − | <th></th> | + | <th>0.060 </th> |
| − | <th></th> | + | <th>0.043</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 1 </th> |
| − | <th></th> | + | <th>0.083 </th> |
| − | <th></th> | + | <th>0.044</th> |
| − | <th></th> | + | <th> 0.023 </th> |
| − | <th></th> | + | <th>0.064 </th> |
| − | <th></th> | + | <th>0.066 </th> |
| − | <th></th> | + | <th>0.035</th> |
| − | <th></th> | + | <th> 0.064 </th> |
| − | <th></th> | + | <th>0.044</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 2</th> |
| − | <th></th> | + | <th> 0.086 </th> |
| − | <th></th> | + | <th>0.045 </th> |
| − | <th></th> | + | <th>0.021 </th> |
| − | <th></th> | + | <th>0.065 </th> |
| − | <th></th> | + | <th>0.067 </th> |
| − | <th></th> | + | <th>0.035 </th> |
| − | <th></th> | + | <th>0.061</th> |
| − | <th></th> | + | <th> 0.042</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 3 </th> |
| − | <th></th> | + | <th>0.079</th> |
| − | <th></th> | + | <th> 0.045 </th> |
| − | <th></th> | + | <th>0.019</th> |
| − | <th></th> | + | <th> 0.065 </th> |
| − | <th></th> | + | <th>0.064 </th> |
| − | <th></th> | + | <th>0.031 </th> |
| − | <th></th> | + | <th>0.058 </th> |
| − | <th></th> | + | <th>0.040</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 4 </th> |
| − | <th></th> | + | <th>0.073 </th> |
| − | <th></th> | + | <th>0.047 </th> |
| − | <th></th> | + | <th>0.022 </th> |
| − | <th></th> | + | <th>0.063 </th> |
| − | <th></th> | + | <th>0.066 </th> |
| − | <th></th> | + | <th>0.033 </th> |
| − | <th></th> | + | <th>0.062 </th> |
| − | <th></th> | + | <th>0.042</th> |
</tr> | </tr> | ||
</table> | </table> | ||
<table> | <table> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Hour 6: </th> |
| − | <th></th> | + | <th>Neg. Control </th> |
| − | <th></th> | + | <th>Pos. Control </th> |
| − | <th></th> | + | <th>Device 1</th> |
| − | <th></th> | + | <th> Device 2</th> |
| − | <th></th> | + | <th> Device 3</th> |
| − | <th></th> | + | <th> Device 4 </th> |
| − | <th></th> | + | <th>Device 5</th> |
| − | <th></th> | + | <th> Device 6</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 1</th> |
| − | <th></th> | + | <th> 0.894</th> |
| − | <th></th> | + | <th> 0.712 </th> |
| − | <th></th> | + | <th>0.153 </th> |
| − | <th></th> | + | <th>0.867 </th> |
| − | <th></th> | + | <th>0.752 </th> |
| − | <th></th> | + | <th>0.212 </th> |
| − | <th></th> | + | <th>0.769 </th> |
| − | <th></th> | + | <th>0.592</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 2 </th> |
| − | <th></th> | + | <th>0.898 </th> |
| − | <th></th> | + | <th>0.709 </th> |
| − | <th></th> | + | <th>0.154 </th> |
| − | <th></th> | + | <th>0.8576</th> |
| − | <th></th> | + | <th> 0.777 </th> |
| − | <th></th> | + | <th>0.211 </th> |
| − | <th></th> | + | <th>0.751</th> |
| − | <th></th> | + | <th> 0.55</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 3 </th> |
| − | <th></th> | + | <th>0.929</th> |
| − | <th></th> | + | <th> 0.729 </th> |
| − | <th></th> | + | <th>0.149 </th> |
| − | <th></th> | + | <th>0.818 </th> |
| − | <th></th> | + | <th>0.764</th> |
| − | <th></th> | + | <th> 0.208 </th> |
| − | <th></th> | + | <th>0.754</th> |
| − | <th></th> | + | <th> 0.559</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 1, Replicate 4 </th> |
| − | <th></th> | + | <th>0.949 </th> |
| − | <th></th> | + | <th>0.754</th> |
| − | <th></th> | + | <th> 0.157 </th> |
| − | <th></th> | + | <th>0.853 </th> |
| − | <th></th> | + | <th>0.758</th> |
| − | <th></th> | + | <th> 0.218 </th> |
| − | <th></th> | + | <th>0.783 </th> |
| − | <th></th> | + | <th>0.565</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 1 </th> |
| − | <th></th> | + | <th>0.779 </th> |
| − | <th></th> | + | <th>0.734 </th> |
| − | <th></th> | + | <th>0.057 </th> |
| − | <th></th> | + | <th>0.817 </th> |
| − | <th></th> | + | <th>0.774 </th> |
| − | <th></th> | + | <th>0.238 </th> |
| − | <th></th> | + | <th>0.718 </th> |
| − | <th></th> | + | <th>0.479</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 2</th> |
| − | <th></th> | + | <th> 0.787 </th> |
| − | <th></th> | + | <th>0.720 </th> |
| − | <th></th> | + | <th>0.057</th> |
| − | <th></th> | + | <th> 0.856 </th> |
| − | <th></th> | + | <th>0.775 </th> |
| − | <th></th> | + | <th>0.232 </th> |
| − | <th></th> | + | <th>0.693</th> |
| − | <th></th> | + | <th> 0.491</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 3 </th> |
| − | <th></th> | + | <th>0.760</th> |
| − | <th></th> | + | <th> 0.747 </th> |
| − | <th></th> | + | <th>0.055</th> |
| − | <th></th> | + | <th> 0.783 </th> |
| − | <th></th> | + | <th>0.762</th> |
| − | <th></th> | + | <th> 0.230 </th> |
| − | <th></th> | + | <th>0.723 </th> |
| − | <th></th> | + | <th>0.521</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th></th> | + | <th>Colony 2, Replicate 4 </th> |
| − | <th></th> | + | <th>0.779 </th> |
| − | <th></th> | + | <th>0.733 </th> |
| − | <th></th> | + | <th>0.053 </th> |
| − | <th></th> | + | <th>0.831 </th> |
| − | <th></th> | + | <th>0.768 </th> |
| − | <th></th> | + | <th>0.245 </th> |
| − | <th></th> | + | <th>0.713 </th> |
| − | <th></th> | + | <th>0.486</th> |
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 20:00, 24 July 2018
INTERLAB
Our team participated in the Fifth International InterLaboratory Measurement Study this year. The interlab study aims to solve the problem of reliability and repeatability in synthetic biology study. This year we were asked to measure green fluorescent protein, which is used as a measurement marker. We followed the experiment protocol strictly to make sure our data is valid. After two weeks lab work, we got the data we need after several attempts.
Calibration Protocol
Three calibration measurement were done by the calibration protocol, including an OD reference point at 600nm, one particle standard curve and one fluorescent standard curve.
★ OD600 reference point ★
| LUDOX CL-X | H2O | |
|---|---|---|
| Replicate 1 | 0.066 | 0.0285 |
| Replicate 2 | 0.059 | 0.029 |
| Replicate 3 | 0.068 | 0.026 |
| Replicate 4 | 0.064 | 0.028 |
| Arith. Mean | 0.064 | 0.028 |
| Corrected Abs600 | 0.036 | |
| Reference OD600 | OD600 | |
| OD600/Abs600 | 1.728 |
Cell measurement
Transformation
Transform Escherichia coli DH5α with these following plasmids:
| Device | Part Number | Location |
|---|---|---|
| Positive control | BBa_R0040 | Well 2D |
| Negative control | Ba_I20270 | Well 2B |
| Test Device 1 | BBa_J364000 | Well 2F |
| Test Device 2 | BBa_J364001 | Well 2H |
| Test Device 3 | BBa_J364002 | Well 2J |
| Test Device 4 | BBa_J364007 | Well 2L |
| Test Device 5 | BBa_J364008 | Well 2N |
| Test Device 6 | BBa_J364009 | Well 2P |
The transformation used E.coli DH5α competent cells bought from Tsingke Biological Technology company, and follow the steps in http://parts.igem.org/Help:2017_DNA_Distribution to use the DNA in the Distribution Kit.
Measurement
★ Fluorescence Raw Readings：★
| Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
|---|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 98.891 | 514.509 | 170.45 | 453.211 | 107.527 | 377.998 | 110.335 | 121.276 | 100.56 |
| Colony 1, Replicate 2 | 87.951 | 520.569 | 155.638 | 443.722 | 103.159 | 353.38 | 99.569 | 117.851 | 97.983 |
| Colony 1, Replicate 3 | 89.665 | 500.61 | 151.21 | 474.651 | 99.334 | 358.843 | 97.329 | 115.674 | 97.686 |
| Colony 1, Replicate 4 | 88.651 | 553.489 | 158.106 | 471.047 | 107.222 | 391.116 | 102.558 | 132.724 | 104.901 |
| Colony 2, Replicate 1 | 94.201 | 799.161 | 176.469 | 396.183 | 105.65 | 452.456 | 105.862 | 129.379 | 100.254 |
| Colony 2, Replicate 2 | 97.455 | 750.414 | 168.71 | 425.555 | 106.286 | 462.598 | 109.024 | 125.311 | 101.914 |
| Colony 2, Replicate 3 | 95.836 | 795.354 | 174.674 | 405.952 | 103.704 | 444.673 | 106.365 | 128.606 | 105.761 |
| Colony 2, Replicate 4 | 100.733 | 804.913 | 203.631 | 370.775 | 117.227 | 506.476 | 113.511 | 125.021 | 105.18 |
| Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
|---|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 142.426 | 1579.487 | 407.651 | 2268.46 | 167.703 | 1014.837 | 144.06 | 365.966 | 130.779 |
| Colony 1, Replicate 2 | 143.238 | 1593.226 | 396.943 | 2328.22 | 174.862 | 1003.434 | 141.231 | 339.499 | 126.638 |
| Colony 1, Replicate 3 | 151.515 | 1699.317 | 396.669 | 2175.501 | 169.71 | 977.6 | 143.762 | 347.711 | 127.911 |
| Colony 1, Replicate 4 | 155.799 | 1783.066 | 406.205 | 2240.128 | 171.295 | 1044.087 | 154.138 | 340.887 | 127.237 |
| Colony 2, Replicate 1 | 150.46 | 2062.959 | 354.432 | 2090.23 | 165.189 | 1268.176 | 150.674 | 320.936 | 120.878 |
| Colony 2, Replicate 2 | 145.579 | 2023.17 | 366.367 | 2169.758 | 167.611 | 1203.787 | 144.458 | 322.957 | 127.103 |
| Colony 2, Replicate 3 | 146.553 | 2101.747 | 353.019 | 1926.808 | 183.724 | 1172.553 | 158.135 | 332.951 | 131.201 |
| Colony 2, Replicate 4 | 157.503 | 2071.459 | 316.402 | 1750.215 | 179.275 | 1253.449 | 155.625 | 327.385 | 137.828 |
★ Abs600 Raw Readings：★
| Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
|---|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 0.1306 | 0.0884 | 0.0767 | 0.1068 | 0.1181 | 0.0753 | 0.1067 | 0.0866 | 0.0486 |
| Colony 1, Replicate 2 | 0.1272 | 0.0906 | 0.0737 | 0.1075 | 0.1184 | 0.0754 | 0.108 | 0.0896 | 0.0497 |
| Colony 1, Replicate 3 | 0.1246 | 0.09 | 0.0747 | 0.1119 | 0.1214 | 0.0731 | 0.1083 | 0.0879 | 0.0464 |
| Colony 1, Replicate 4 | 0.1225 | 0.0872 | 0.0743 | 0.1109 | 0.1247 | 0.0746 | 0.1079 | 0.0903 | 0.0476 |
| Colony 2, Replicate 1 | 0.1301 | 0.0907 | 0.0701 | 0.1109 | 0.1132 | 0.0813 | 0.1103 | 0.0909 | 0.0468 |
| Colony 2, Replicate 2 | 0.1327 | 0.0919 | 0.0685 | 0.1125 | 0.1145 | 0.0822 | 0.1081 | 0.0896 | 0.0472 |
| Colony 2, Replicate 3 | 0.1294 | 0.0952 | 0.0693 | 0.1151 | 0.1139 | 0.0809 | 0.1084 | 0.0898 | 0.05 |
| Colony 2, Replicate 4 | 0.1202 | 0.0941 | 0.0692 | 0.1105 | 0.1134 | 0.0805 | 0.1092 | 0.089 | 0.0472 |
| Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
|---|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 0.9386 | 0.7564 | 0.1978 | 0.911 | 0.7963 | 0.2566 | 0.8131 | 0.6363 | 0.0444 |
| Colony 1, Replicate 2 | 0.9413 | 0.7528 | 0.1974 | 0.9012 | 0.8211 | 0.2547 | 0.7951 | 0.5996 | 0.0438 |
| Colony 1, Replicate 3 | 0.9745 | 0.7748 | 0.1951 | 0.8642 | 0.81 | 0.2536 | 0.7994 | 0.6044 | 0.0458 |
| Colony 1, Replicate 4 | 0.9932 | 0.7975 | 0.2009 | 0.8969 | 0.8021 | 0.2622 | 0.8266 | 0.6092 | 0.0438 |
| Colony 2, Replicate 1 | 0.8246 | 0.78 | 0.1031 | 0.8625 | 0.8193 | 0.2834 | 0.7633 | 0.5251 | 0.0457 |
| Colony 2, Replicate 2 | 0.8327 | 0.7665 | 0.1031 | 0.9018 | 0.8211 | 0.2781 | 0.7394 | 0.5374 | 0.0462 |
| Colony 2, Replicate 3 | 0.8062 | 0.7927 | 0.1005 | 0.829 | 0.8082 | 0.276 | 0.7689 | 0.5668 | 0.0458 |
| Colony 2, Replicate 4 | 0.8262 | 0.7805 | 0.1005 | 0.8787 | 0.8156 | 0.2928 | 0.7604 | 0.5332 | 0.0477 |
★ uM Fluorescein / OD ★
| Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 |
|---|---|---|---|---|---|---|---|---|
| olony 1, Replicate 1 | -0.002 | 0.872 | 0.208 | 0.508 | 0.008 | 0.871 | 0.014 | 0.046 |
| Colony 1, Replicate 2 | -0.011 | 0.866 | 0.201 | 0.501 | 0.006 | 0.833 | 0.002 | 0.042 |
| Colony 1, Replicate 3 | -0.009 | 0.775 | 0.159 | 0.482 | 0.002 | 0.820 | 0.000 | 0.036 |
| Colony 1, Replicate 4 | -0.018 | 0.950 | 0.167 | 0.485 | 0.003 | 0.889 | -0.003 | 0.055 |
| Colony 2, Replicate 1 | -0.006 | 1.334 | 0.274 | 0.387 | 0.007 | 0.856 | 0.007 | 0.055 |
| Colony 2, Replicate 2 | -0.004 | 1.216 | 0.263 | 0.415 | 0.005 | 0.864 | 0.010 | 0.046 |
| Colony 2, Replicate 3 | -0.010 | 1.279 | 0.299 | 0.387 | -0.003 | 0.919 | 0.001 | 0.048 |
| Colony 2, Replicate 4 | -0.005 | 1.251 | 0.375 | 0.352 | 0.015 | 1.010 | 0.011 | 0.040 |
| Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 |
|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 0.001 | 0.171 | 0.151 | 0.207 | 0.004 | 0.349 | 0.001 | 0.033 |
| Colony 1, Replicate 2 | 0.002 | 0.173 | 0.148 | 0.215 | 0.005 | 0.348 | 0.002 | 0.032 |
| Colony 1, Replicate 3 | 0.002 | 0.181 | 0.151 | 0.210 | 0.005 | 0.343 | 0.002 | 0.033 |
| Colony 1, Replicate 4 | 0.003 | 0.184 | 0.149 | 0.208 | 0.005 | 0.352 | 0.003 | 0.032 |
| Colony 2, Replicate 1 | 0.003 | 0.222 | 0.341 | 0.202 | 0.005 | 0.405 | 0.003 | 0.035 |
| Colony 2, Replicate 2 | 0.002 | 0.221 | 0.352 | 0.200 | 0.004 | 0.389 | 0.002 | 0.033 |
| Colony 2, Replicate 3 | 0.002 | 0.221 | 0.340 | 0.192 | 0.006 | 0.379 | 0.003 | 0.032 |
| Colony 2, Replicate 4 | 0.002 | 0.221 | 0.283 | 0.163 | 0.005 | 0.382 | 0.002 | 0.033 |
★ Net Fluorescein a.u. ★
| Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 |
|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | -1.67 | 413.95 | 69.89 | 352.65 | 6.97 | 277.44 | 9.77 | 20.72 |
| Colony 1, Replicate 2 | -10.03 | 422.59 | 57.66 | 345.74 | 5.18 | 255.40 | 1.59 | 19.87 |
| Colony 1, Replicate 3 | -8.02 | 402.92 | 53.52 | 376.97 | 1.65 | 261.16 | -0.36 | 17.99 |
| Colony 1, Replicate 4 | -16.25 | 448.59 | 53.21 | 366.15 | 2.32 | 286.22 | -2.34 | 27.82 |
| Colony 2, Replicate 1 | -6.05 | 698.91 | 76.22 | 295.93 | 5.40 | 352.20 | 5.61 | 29.13 |
| Colony 2, Replicate 2 | -4.46 | 648.50 | 66.80 | 323.64 | 4.37 | 360.68 | 7.11 | 23.40 |
| Colony 2, Replicate 3 | -9.93 | 689.59 | 68.91 | 300.19 | -2.06 | 338.91 | 0.60 | 22.85 |
| Colony 2, Replicate 4 | -4.45 | 699.73 | 98.45 | 265.60 | 12.05 | 401.30 | 8.33 | 19.84 |
| Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 |
|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 11.65 | 1448.71 | 276.87 | 2137.68 | 36.92 | 884.06 | 13.28 | 235.19 |
| Colony 1, Replicate 2 | 16.60 | 1466.59 | 270.31 | 2201.58 | 48.22 | 876.80 | 14.59 | 212.86 |
| Colony 1, Replicate 3 | 23.60 | 1571.41 | 268.76 | 2047.59 | 41.80 | 849.69 | 15.85 | 219.80 |
| Colony 1, Replicate 4 | 28.56 | 1655.83 | 278.97 | 2112.89 | 44.06 | 916.85 | 26.90 | 213.65 |
| Colony 2, Replicate 1 | 29.58 | 1942.08 | 233.55 | 1969.35 | 44.31 | 1147.30 | 29.80 | 200.06 |
| Colony 2, Replicate 2 | 18.48 | 1896.07 | 239.26 | 2042.66 | 40.51 | 1076.68 | 17.36 | 195.85 |
| Colony 2, Replicate 3 | 15.35 | 1970.55 | 221.82 | 1795.61 | 52.52 | 1041.35 | 26.93 | 201.75 |
| Colony 2, Replicate 4 | 19.68 | 1933.63 | 178.57 | 1612.39 | 41.45 | 1115.62 | 17.80 | 189.56 |
★ Net Abs 600 ★
| Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 |
|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 0.082 | 0.040 | 0.028 | 0.058 | 0.070 | 0.027 | 0.058 | 0.038 |
| Colony 1, Replicate 2 | 0.078 | 0.041 | 0.024 | 0.058 | 0.069 | 0.026 | 0.058 | 0.040 |
| Colony 1, Replicate 3 | 0.078 | 0.044 | 0.028 | 0.066 | 0.075 | 0.027 | 0.062 | 0.042 |
| Colony 1, Replicate 4 | 0.075 | 0.040 | 0.027 | 0.063 | 0.077 | 0.027 | 0.060 | 0.043 |
| Colony 2, Replicate 1 | 0.083 | 0.044 | 0.023 | 0.064 | 0.066 | 0.035 | 0.064 | 0.044 |
| Colony 2, Replicate 2 | 0.086 | 0.045 | 0.021 | 0.065 | 0.067 | 0.035 | 0.061 | 0.042 |
| Colony 2, Replicate 3 | 0.079 | 0.045 | 0.019 | 0.065 | 0.064 | 0.031 | 0.058 | 0.040 |
| Colony 2, Replicate 4 | 0.073 | 0.047 | 0.022 | 0.063 | 0.066 | 0.033 | 0.062 | 0.042 |
| Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 |
|---|---|---|---|---|---|---|---|---|
| Colony 1, Replicate 1 | 0.894 | 0.712 | 0.153 | 0.867 | 0.752 | 0.212 | 0.769 | 0.592 |
| Colony 1, Replicate 2 | 0.898 | 0.709 | 0.154 | 0.8576 | 0.777 | 0.211 | 0.751 | 0.55 |
| Colony 1, Replicate 3 | 0.929 | 0.729 | 0.149 | 0.818 | 0.764 | 0.208 | 0.754 | 0.559 |
| Colony 1, Replicate 4 | 0.949 | 0.754 | 0.157 | 0.853 | 0.758 | 0.218 | 0.783 | 0.565 |
| Colony 2, Replicate 1 | 0.779 | 0.734 | 0.057 | 0.817 | 0.774 | 0.238 | 0.718 | 0.479 |
| Colony 2, Replicate 2 | 0.787 | 0.720 | 0.057 | 0.856 | 0.775 | 0.232 | 0.693 | 0.491 |
| Colony 2, Replicate 3 | 0.760 | 0.747 | 0.055 | 0.783 | 0.762 | 0.230 | 0.723 | 0.521 |
| Colony 2, Replicate 4 | 0.779 | 0.733 | 0.053 | 0.831 | 0.768 | 0.245 | 0.713 | 0.486 |