Difference between revisions of "Team:SDU-CHINA/Experiments"

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<head>
 
<head>
 
<meta charset="utf-8" />
 
<meta charset="utf-8" />
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    <link rel="stylesheet" href="https://2018.igem.org/Team:SDU-CHINA/bootstrap337?action=raw&ctype=text/css">
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<link rel="stylesheet" href="https://2018.igem.org/Team:SDU-CHINA/bootstrap337?action=raw&ctype=text/css">
 +
 
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<link href="https://2018.igem.org/wiki/index.php?title=Template:SDU-China/slickcss190&action=raw&ctype=text/css" rel="stylesheet">
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<link href="https://2018.igem.org/wiki/index.php?title=Template:SDU-China/slickthemecss190&action=raw&ctype=text/css" rel="stylesheet">
 
   <script src="https://2018.igem.org/Team:SDU-China/jquery211?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2018.igem.org/Team:SDU-China/jquery211?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2018.igem.org/Team:SDU-China/bootjs?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2018.igem.org/Team:SDU-China/bootjs?action=raw&ctype=text/javascript"></script>
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    <link rel="stylesheet" type="text/css" href="font-awesome-4.7.0/css/font-awesome.min.css">
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  <script src="https://2018.igem.org/Team:SDU-China/jquery311?action=raw&ctype=text/javascript"></script>
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 +
 
 
     <style>
 
     <style>
 
     #contentSub, #footer-box, #catlinks, #search-controls, #p-logo,
 
     #contentSub, #footer-box, #catlinks, #search-controls, #p-logo,
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         width: 100%;  
 
         width: 100%;  
 
         height: 100%;  
 
         height: 100%;  
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         background-color: transparent;
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         background-color: transparent;
 
     }
 
     }
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li { list-style: none; }b
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#menu a { text-decoration: none; }
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#menu a:link, a:visited, a:active { color: black; }
+
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#menu a:hover { color: #c1d9ff; }
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table { margin: 0 auto; }
 
table { margin: 0 auto; }
 
td { text-align: center }
 
td { text-align: center }
  
 
#content{
 
#content{
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margin: 0px;
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    margin-top: -22px;
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padding: 0px;
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    padding: 0px;
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background-color: #E6E6F2;
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    background-color: #E6E6F2;
 
         width: 100%;  
 
         width: 100%;  
 
         height: 100%;
 
         height: 100%;
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}
 
}
  
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#header, #footer {
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#footer,#header {
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background: linear-gradient(to right, #005AB5, #007500);
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    background: linear-gradient(to right, #005AB5, #007500);
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background: -webkit-linear-gradient(to right, #005AB5, #007500);
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    background: -webkit-linear-gradient(to right, #005AB5, #007500);
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background: -moz-linear-gradient(to right, #005AB5, #007500);
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    background: -moz-linear-gradient(to right, #005AB5, #007500);
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background: -o-linear-gradient(to right, #005AB5, #007500);
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    background: -o-linear-gradient(to right, #005AB5, #007500);
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float: left;
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    float: left;
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width: 100%;
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    width: 100%;
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padding: 1% 0%;
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         margin: 0 0px !important;
 
         margin: 0 0px !important;
 
}
 
}
  
 
#logo {
 
#logo {
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    float: left;
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position: relative;
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    position: relative;
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margin-left:1%;
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    margin-left:1%;
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display: block !important;
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    display: block !important;
 
}
 
}
 
#logo img {  width:150px;} /*???*/
 
#logo img {  width:150px;} /*???*/
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#footer { height: 180px;  color: white; }
 
 
#sponsors {
 
#sponsors {
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float: left;
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margin-left: 15px;
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    margin-left: 15px;
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margin-top: 2px;
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    margin-top: 2px;
 
}
 
}
 
#address {
 
#address {
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text-align: center;
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float: right;
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margin-right: 20px;
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margin-left: 30px;
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margin-top: 2px;
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}
 
}
 
#contact {
 
#contact {
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text-align: center;
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float: right;
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position: relative;
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margin-right: 30px;
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margin-top: 2px;
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    margin-top: 2px;
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width: 230px;
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    width: 230px;
 
}
 
}
  
  
  
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#section {clear: both;}
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#section {clear: both;
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width:100%;
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margin-top:-20px;
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padding: 133px auto 0 auto;
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overflow: hidden;
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background-color:white !important;}
 
#main-title {
 
#main-title {
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font-size: 60px;
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color: #4b5ea4;
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font-family: sans-serif;  
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text-align: center;
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font-weight: lighter;
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margin: 0;
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padding: 30px 0px 0px 0px;
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    padding: 30px 0px 0px 0px;
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margin: 0px 67px 0px 65px;
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    margin: 0px 67px 0px 65px;
 
}
 
}
 
.sublinks { text-align: center; }
 
.sublinks { text-align: center; }
 
.sublinks h2 {
 
.sublinks h2 {
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margin: 20px 40px;
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    margin: 20px 40px;
 
}
 
}
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.sublinks a:link, .sublinks a:visited, .sublinks a:active, .p-top a:link, .p-top a:visited, .p-top a:active { color: #8298ec;  font-family: sans-serif;  font-weight: lighter; }
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.sublinks a:link, .sublinks a:visited, .sublinks a:active, .p-top a:link, .p-top a:visited, .p-top a:active { color: #4b5ea4;  font-family: sans-serif;  font-weight: lighter; }
 
.sub-title {
 
.sub-title {
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font-family: sans-serif;
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color: white;
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}
 
}
 
.conteudo {
 
.conteudo {
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     line-height: 1.5;
 
     line-height: 1.5;
 
     clear: both;
 
     clear: both;
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}
 
}
 
.p-top {
 
.p-top {
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margin: 5px 0 0 0;
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font: 25px sans-serif;
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transform: translateY(80%);
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}
 
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p {
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p.partone {
 
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}
 
}
 
p {
 
p {
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h4{
 
h4{
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display: inline-block;
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    display: inline-block;
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display:-webkit-inline-block; /* Safari, Chrome, and Opera */
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    display:-webkit-inline-block; /* Safari, Chrome, and Opera */
 
     display: -moz-inline-block;/* Firefox */
 
     display: -moz-inline-block;/* Firefox */
 
     display: -o-inline-block;
 
     display: -o-inline-block;
 
     width: 700px;
 
     width: 700px;
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}
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h1{
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 +
}
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*{margin: 0;padding: 0;}
 +
         
 +
  #box div{
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    display: block;
 +
    width: 600px;
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    height: 500px;
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    margin: 0 auto;
 +
  }
 +
  #box img{
 +
    width: 600px;
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    height: 500px;
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    display: table-cell;
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    vertical-align: middle;
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  text-align: center;
 +
 +
  }
 +
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ul li{
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}
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ol li{​
 +
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        list-style-type:decimal;
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        list-style-position:inside ;
 +
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        font-size: 15px;
 +
 +
}
 +
ol{
 +
  padding-left: 10px ;
 +
  padding-bottom: 40px;
 
}
 
}
 
#header{
 
#header{

Revision as of 12:48, 7 October 2018

Protocols

Transformation

E. coli transformation protocol
Please click on link to view the protocol for E. coli transformation.

Gel extraction

Gel extraction protocol
Please click on link to view the protocol for gel extraction protocol.

Gibson assembly

Gibson assembly protocol
Please click on link to view the protocol for gibson assembly.

Gel electrophoresis

Agarose gel electrophoresis protocol
Please click on link to view the protocol for gel electrophoresis.

PCR(Vazyme)

PCR protocol(Vazyme)
Please click on link to view the protocol for PCR(Vazyme).

PCR(Trans)

PCR protocol(Trans)
Please click on link to view the protocol for PCR(Trans).

ABM assembly

ABM assembly protocol
Please click on link to view the protocol for ABM assembly.

Preparation of competent cells

Protocol of preparing competent cells
Please click on link to view the protocol for preparing competent cells.

Purification of bacteria plasmids

Protocol of purifying bacteria plasmids
Please click on link to view the protocol for purifying bacteria plasmids.

PCR extraction

PCR extraction protocol
Please click on link to view the protocol for PCR extraction.

Characterization of CcaS-CcaR system

Characterization of CcaS-CcaR system
Please click on link to view the protocol for charactrizing CcaS-CcaR system.

Characterization of crRNA

Characterization of crRNA
Please click on link to view the charactrizing crRNA.

Characterization of split RNAP

Characterization of split RNAP
Please click on link to view the protocol for charactrizing split RNAP.

Measurement of PHB production

Measurement of PHB production
Please click on link to view the protocol for measuring PHB production.

Nile red stain

Nile red stain protocol
Please click on link to view the protocol for Nile red stain.

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