Difference between revisions of "Team:SDU-CHINA/Improve"

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<div class="column full_size judges-will-not-evaluate">
 
<h3>★  ALERT! </h3>
 
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
 
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
 
<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement.  Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
 
  
The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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<br><br>
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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</head>
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<body>
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<div class="body">
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    <div id="header">
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    <!--LOGO LINK--->
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    <div id="logo"><a href="https://2018.igem.org/Team:SDU-CHINA"><img src="https://static.igem.org/mediawiki/2018/7/72/T--SDU-China--duihui.jpg" alt="LOGO" /></a></div>
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    <div id="menu">
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        <ul>
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            <li>
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                <a href="#">PROJECT &#9662;</a>
 +
                <ul>
 +
                    <!--links-->
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                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Description">Description</a></li>
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                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Design">Design</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Results">Results</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Demonstrate">Demonstrate</a></li>
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                </ul>
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            <li>
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                <a href="#">WETLAB &#9662;</a>
 +
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                    <li><a href="#">Protocols</a></li>
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                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Notebook">Notebook</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Safety">Safety</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
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                <a href="#">DRYLAB &#9662;</a>
 +
                <ul>
 +
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                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Model">Model</a></li>
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                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Hardware">Hardware</a></li>
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 +
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            <!--links-->
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            <li><a href="https://2018.igem.org/Team:SDU-CHINA/InterLab">INTERLAB</a></li>
 +
            <li><a href="#">PARTS &#9662;</a>
 +
            <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Parts">Parts</a></li>
 +
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Improve">Improve</a></li>
 +
                   
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                </ul>
 +
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 +
            <li><a href="https://2018.igem.org/Team:SDU-CHINA/Human_Practices">HUMAN PRACTICES &#9662;</a>
 +
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                    <!--links-->
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                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Human_Practices#Silver">Silver</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Human_Practices#integrated">Gold & Integrated</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Public_Engagement">Engagement</a></li>
 +
                </ul>
 +
                </li>
 +
        </ul>
 +
    </div>
 
</div>
 
</div>
  
 +
<div id="section">
 +
    <div class="conteudo">
 +
        <h1 id="main-title">Improve</h1>
 +
 +
  <b>Advanced CcaS with PSA domain knocked out with an opposite response to green/red light.</b>
 +
CcaS is a membrane protein functioning in CcaS/CcaR two-component system in response to green/red light. Under green light, the system and activate the transcription and expression of the specific genes downstream a proper promoter named PcpcG2, while the expression of the genes can be repressed when exposed to red light. This year, we were inspired by Professor Sode’ work of making a modification of CcaS’s structure and functions. And we deleted 858 base pairs in gene coding for CcaS, which means we removed the PSA domain in CcaS protein. When we characterized this new part, we surprised to find that the new CcaS had an opposite response to green/red light, compared to the original one.
 +
放几张表征的图,然后附上part的链接http://parts.igem.org/Part:BBa_K2627000
 +
<img src="https://static.igem.org/mediawiki/2018/e/e2/T--SDU-China--chara.png" alt="">
 +
 +
        </div>
 +
    </div>
 +
 +
<div id="footer">
 +
    <div id="sponsors">
 +
        <img src="https://static.igem.org/mediawiki/2018/a/ab/T--SDU-CHINA--Sponsors0.svg" alt="SPONSORS" usemap="#Map-Sponsors" />
 +
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 +
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            <area title="MATHWOAR" href="https://www.mathworks.com/" shape="rect" coords="247,0,440,60" />
 +
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 +
        </map>
 +
    </div>
 +
    <div id="contact">
 +
        <div><a href="mailto:sdubioigem@163.com">mailto:sdubioigem@163.com</a></div>
 +
        <div><a href="https://www.instagram.com/sdubiochina_2018/">www.instagram.com/sdubiochina_2018/</a></div>
 +
    </div>
 +
</div>
 +
</div>
  
  
 +
</body>
 
</html>
 
</html>

Revision as of 07:42, 11 October 2018

Improve

Advanced CcaS with PSA domain knocked out with an opposite response to green/red light. CcaS is a membrane protein functioning in CcaS/CcaR two-component system in response to green/red light. Under green light, the system and activate the transcription and expression of the specific genes downstream a proper promoter named PcpcG2, while the expression of the genes can be repressed when exposed to red light. This year, we were inspired by Professor Sode’ work of making a modification of CcaS’s structure and functions. And we deleted 858 base pairs in gene coding for CcaS, which means we removed the PSA domain in CcaS protein. When we characterized this new part, we surprised to find that the new CcaS had an opposite response to green/red light, compared to the original one. 放几张表征的图,然后附上part的链接http://parts.igem.org/Part:BBa_K2627000