Difference between revisions of "Team:Edinburgh OG/Parts"

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     <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
 
     <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
 
     <td>1459bp</td>
 
     <td>1459bp</td>
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     <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> Sequence is codon optimised for E.coli</td>
+
     <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>. Sequence is codon optimised for E.coli.</td>
 
       </tr>
 
       </tr>
 
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Revision as of 18:47, 16 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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Parts

. Part of PhaR is codon optimised for E.coli
Registry Number Brief Description Key Details Length Sequence information
BBa_K2739005 proR-PhaR-Pha promoter-PhaP The phasin autoregulation system with its native promoter + phasin with its native promoter 1459bp Original Sequence obtained from: NCBI(AM260479.1). Sequence is codon optimised for E.coli.
BBa_K2739006 proR-PhaR-proP-PhaP-HlyA The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter 1646bp Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501
BBa_K2739007 LacP-RBS-PhaP-HlyA-Terminator Phasin with HlyA signal peptide under the control of Lac promoter 1139bp This part is developed from: BBa_K390501
BBa_K2739009 Hybrid promoter-PhaCAB-Bktb Co-expression of Bktb with PHA operon 5462bp Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli
BBa_K2739010 Hybrid promoter-PhaCB-Bktb Bktb in replacing the phaA in PHA operon 4252bp Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli