Difference between revisions of "Team:Edinburgh OG/Parts"

Revision as of 18:49, 16 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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 	      <td>Bktb in replacing the phaA in PHA operon</td>
 	      <td>4252bp</td>
-	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and BBa_K1149051. Part of Bktb is codon optimised for E.coli.</td>
+	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for E.coli.</td>
 	   </tr>
      </tbody>

Registry Number	Brief Description	Key Details	Length	Sequence information
BBa_K2739005	proR-PhaR-Pha promoter-PhaP	The phasin autoregulation system with its native promoter + phasin with its native promoter	1459bp	Original Sequence obtained from: NCBI(AM260479.1). Sequence is codon optimised for E.coli.
BBa_K2739006	proR-PhaR-proP-PhaP-HlyA	The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter	1646bp	Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501. Part of PhaR is codon optimised for E.coli.
BBa_K2739007	LacP-RBS-PhaP-HlyA-Terminator	Phasin with HlyA signal peptide under the control of Lac promoter	1139bp	This part is developed from: BBa_K390501.
BBa_K2739009	Hybrid promoter-PhaCAB-Bktb	Co-expression of Bktb with PHA operon	5462bp	Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.
BBa_K2739010	Hybrid promoter-PhaCB-Bktb	Bktb in replacing the phaA in PHA operon	4252bp	Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.