Difference between revisions of "Team:Edinburgh OG/Parts"

Latest revision as of 01:22, 18 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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       	 <td>The phasin autoregulation system with its native promoter + phasin with its native promoter</td>
       	 <td>1459bp</td>
-      	 <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>. Sequence is codon optimised for E.coli.</td>
+      	 <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>. Sequence is codon optimised for <em>E.coli</em>.</td>
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 	      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with the phasin native promoter </td>
 	      <td>1646bp</td>
-	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> and <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>. Part of PhaR is codon optimised for E.coli.</td>
+	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> and <a href="http://parts.igem.org/Part:BBa_K390501">BBa_K390501</a>. Part of PhaR is codon optimised for <em>E.coli</em>.</td>
 	   </tr>
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@@ Line 53: / Line 53: @@
 	      <td>Co-expression of Bktb with PHA operon</td>
 	      <td>5462bp</td>
-	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for E.coli.</td>
+	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for <em>E.coli</em>.</td>
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@@ Line 60: / Line 60: @@
 	      <td>Bktb in replacing the phaA in PHA operon</td>
 	      <td>4252bp</td>
-	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for E.coli.</td>
+	      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI(NC_015726.1)</a> and <a href="http://parts.igem.org/Part:BBa_K1149051">BBa_K1149051</a>. Part of Bktb is codon optimised for <em>E.coli</em>.</td>
 	   </tr>
      </tbody>

Registry Number	Brief Description	Key Details	Length	Sequence information
BBa_K2739005	proR-PhaR-Pha promoter-PhaP	The phasin autoregulation system with its native promoter + phasin with its native promoter	1459bp	Original Sequence obtained from: NCBI(AM260479.1). Sequence is codon optimised for E.coli.
BBa_K2739006	proR-PhaR-proP-PhaP-HlyA	The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with the phasin native promoter	1646bp	Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501. Part of PhaR is codon optimised for E.coli.
BBa_K2739007	LacP-RBS-PhaP-HlyA-Terminator	Phasin with HlyA signal peptide under the control of Lac promoter	1139bp	This part is developed from: BBa_K390501.
BBa_K2739009	Hybrid promoter-PhaCAB-Bktb	Co-expression of Bktb with PHA operon	5462bp	Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.
BBa_K2739010	Hybrid promoter-PhaCB-Bktb	Bktb in replacing the phaA in PHA operon	4252bp	Original Sequence obtained from: NCBI(NC_015726.1) and BBa_K1149051. Part of Bktb is codon optimised for E.coli.