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− | <img src="https://static.igem.org/mediawiki/2018/f/fc/T--ZJUT-China--Particle_Standard_Curve_%28log_scale%29e.png" alt="Particle_Standard_Curve_(log_scale)e"> | + | <img src="https://static.igem.org/mediawiki/2018/f/fc/T--ZJUT-China--Particle_Standard_Curve_%28log_scale%29e.png" width="400"alt="Particle_Standard_Curve_(log_scale)e"> |
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<div class="column judges-will-not-evaluate middle" > | <div class="column judges-will-not-evaluate middle" > |
Revision as of 09:25, 23 July 2018
★ OD600 reference point ★
LUDOX CL-X | H2O | |
---|---|---|
Replicate 1 | 0.066 | 0.0285 |
Replicate 2 | 0.059 | 0.029 |
Replicate 3 | 0.068 | 0.026 |
Replicate 4 | 0.064 | 0.028 |
Arith. Mean | 0.064 | 0.028 |
Corrected Abs600 | 0.036 | |
Reference OD600 | OD600 | |
OD600/Abs600 | 1.728 |
★ Fluorescence Raw Readings:★
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 98.891 | 514.509 | 170.45 | 453.211 | 107.527 | 377.998 | 110.335 | 121.276 | 100.56 |
Colony 1, Replicate 2 | 87.951 | 520.569 | 155.638 | 443.722 | 103.159 | 353.38 | 99.569 | 117.851 | 97.983 |
Colony 1, Replicate 3 | 89.665 | 500.61 | 151.21 | 474.651 | 99.334 | 358.843 | 97.329 | 115.674 | 97.686 |
Colony 1, Replicate 4 | 88.651 | 553.489 | 158.106 | 471.047 | 107.222 | 391.116 | 102.558 | 132.724 | 104.901 |
Colony 2, Replicate 1 | 94.201 | 799.161 | 176.469 | 396.183 | 105.65 | 452.456 | 105.862 | 129.379 | 100.254 |
Colony 2, Replicate 2 | 97.455 | 750.414 | 168.71 | 425.555 | 106.286 | 462.598 | 109.024 | 125.311 | 101.914 |
Colony 2, Replicate 3 | 95.836 | 795.354 | 174.674 | 405.952 | 103.704 | 444.673 | 106.365 | 128.606 | 105.761 |
Colony 2, Replicate 4 | 100.733 | 804.913 | 203.631 | 370.775 | 117.227 | 506.476 | 113.511 | 125.021 | 105.18 |
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 142.426 | 1579.487 | 407.651 | 2268.46 | 167.703 | 1014.837 | 144.06 | 365.966 | 130.779 |
Colony 1, Replicate 2 | 143.238 | 1593.226 | 396.943 | 2328.22 | 174.862 | 1003.434 | 141.231 | 339.499 | 126.638 |
Colony 1, Replicate 3 | 151.515 | 1699.317 | 396.669 | 2175.501 | 169.71 | 977.6 | 143.762 | 347.711 | 127.911 |
Colony 1, Replicate 4 | 155.799 | 1783.066 | 406.205 | 2240.128 | 171.295 | 1044.087 | 154.138 | 340.887 | 127.237 |
Colony 2, Replicate 1 | 150.46 | 2062.959 | 354.432 | 2090.23 | 165.189 | 1268.176 | 150.674 | 320.936 | 120.878 |
Colony 2, Replicate 2 | 145.579 | 2023.17 | 366.367 | 2169.758 | 167.611 | 1203.787 | 144.458 | 322.957 | 127.103 |
Colony 2, Replicate 3 | 146.553 | 2101.747 | 353.019 | 1926.808 | 183.724 | 1172.553 | 158.135 | 332.951 | 131.201 |
Colony 2, Replicate 4 | 157.503 | 2071.459 | 316.402 | 1750.215 | 179.275 | 1253.449 | 155.625 | 327.385 | 137.828 |
★ Abs600 Raw Readings:★
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.1306 | 0.0884 | 0.0767 | 0.1068 | 0.1181 | 0.0753 | 0.1067 | 0.0866 | 0.0486 |
Colony 1, Replicate 2 | 0.1272 | 0.0906 | 0.0737 | 0.1075 | 0.1184 | 0.0754 | 0.108 | 0.0896 | 0.0497 |
Colony 1, Replicate 3 | 0.1246 | 0.09 | 0.0747 | 0.1119 | 0.1214 | 0.0731 | 0.1083 | 0.0879 | 0.0464 |
Colony 1, Replicate 4 | 0.1225 | 0.0872 | 0.0743 | 0.1109 | 0.1247 | 0.0746 | 0.1079 | 0.0903 | 0.0476 |
Colony 2, Replicate 1 | 0.1301 | 0.0907 | 0.0701 | 0.1109 | 0.1132 | 0.0813 | 0.1103 | 0.0909 | 0.0468 |
Colony 2, Replicate 2 | 0.1327 | 0.0919 | 0.0685 | 0.1125 | 0.1145 | 0.0822 | 0.1081 | 0.0896 | 0.0472 |
Colony 2, Replicate 3 | 0.1294 | 0.0952 | 0.0693 | 0.1151 | 0.1139 | 0.0809 | 0.1084 | 0.0898 | 0.05 |
Colony 2, Replicate 4 | 0.1202 | 0.0941 | 0.0692 | 0.1105 | 0.1134 | 0.0805 | 0.1092 | 0.089 | 0.0472 |
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.9386 | 0.7564 | 0.1978 | 0.911 | 0.7963 | 0.2566 | 0.8131 | 0.6363 | 0.0444 |
Colony 1, Replicate 2 | 0.9413 | 0.7528 | 0.1974 | 0.9012 | 0.8211 | 0.2547 | 0.7951 | 0.5996 | 0.0438 |
Colony 1, Replicate 3 | 0.9745 | 0.7748 | 0.1951 | 0.8642 | 0.81 | 0.2536 | 0.7994 | 0.6044 | 0.0458 |
Colony 1, Replicate 4 | 0.9932 | 0.7975 | 0.2009 | 0.8969 | 0.8021 | 0.2622 | 0.8266 | 0.6092 | 0.0438 |
Colony 2, Replicate 1 | 0.8246 | 0.78 | 0.1031 | 0.8625 | 0.8193 | 0.2834 | 0.7633 | 0.5251 | 0.0457 |
Colony 2, Replicate 2 | 0.8327 | 0.7665 | 0.1031 | 0.9018 | 0.8211 | 0.2781 | 0.7394 | 0.5374 | 0.0462 |
Colony 2, Replicate 3 | 0.8062 | 0.7927 | 0.1005 | 0.829 | 0.8082 | 0.276 | 0.7689 | 0.5668 | 0.0458 |
Colony 2, Replicate 4 | 0.8262 | 0.7805 | 0.1005 | 0.8787 | 0.8156 | 0.2928 | 0.7604 | 0.5332 | 0.0477 |
★ Assumed plate well pattern:★
A1 | A2 | A3 | A4 | A5 | A6 | A7 | A8 | A9 |
---|---|---|---|---|---|---|---|---|
B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | B9 |
C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 |
D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 |
E1 | E2 | E3 | E4 | E5 | E6 | E7 | E8 | E9 |
F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 |
G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | G9 |
H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 |