Team:UNSW Australia/Notebook
Notebook
All aspects of our lab work were documented using Benchling, and we have also displayed a portion of the relevant information on this page.
Click on the buttons below to toggle the lab notes for the respective sections.
Week 1
30th April - 6th May
Cloning
Luria Broth (LB) media was made and antibiotic plates were poured out (ampicillin and kanamycin).
A small-scale grow up of NEB Turbo colonies was prepared by Dr Daniel Lorenz Winter. The cultures were miniprepped and the concentrations of the plasmids were recorded.
Homemade Gibson Mastermix was made.
Week 2
7th May - 13th May
Cloning
pETDuet-1 and pRSFDUet-1 plasmids were PCR linearised and a DpnI digest was performed. An agarose el was ran to check band sizes.
Week 3
14th May - 20th May
Cloning
- Gibson cloning was performed on aPFD, aPFD-SpyC, bPFD, bPFD-SnpC, IaaM-SnpT, IaaH-SpyT and mVenus-SpyT. Recombinant plasmids were transformed into cells, colony PCR (cPCR) was performed and the corresponding colonies were grown and miniprepped.
Gel was unsuccessful.