Team:Edinburgh OG/Improve

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

 

 

 

 

 

Our Best Improved Part ! ! !
Registry Number Brief Description Key Details Length Sequence information
BBa_K2739000 ProR-PhaR The phasin autoregulation system with native promoter 658bp Original Sequence obtained from: NCBI(AM260479.1). Sequence is codon optimised for E.coli.
BBa_K108015 The previous PhaR The phasin autoregulation system 552bp Original Sequence obtained from: NCBI(AM260479.1). No codon optimisation for E.coli.

This composite part has allow us to investigate A) its relation in PHA production through PHA operon B) its function on PHA production with Phasin C) Assess the secretion function of Phasin-HlyA is compatible with phaR and if the improvement of phaR of PHA production is remained in this parts composition.

The function part of the promoter is more properly selected and The coding sequence of PhaR is codon optimised to E.coli