Team:UCAS-China/Composite Part

BASIC PART

Type Parts Name Number Description
promoter laclq35 BBa_K2598007 a promoter promotes expression of YF1 and fixJ
promoter PCGG BBa_K2598023  a promoter that can be regulated by the full-functional RNA polymerase consisting of T7 RNAP sigma fragment T3 and T7 RNAP core fragment and promotes blue light output
promoter PT3 BBa_K2598026  a promoter that can be regulated by the full-functional RNA polymerase consisting of T7 RNAP sigma fragment CGG and T7 RNAP core fragment and promotes green light output
promoter PK1F BBa_K2598030 a promoter that can be regulated by the full-functional RNA polymerase consisting of T7 RNAP sigma fragment K1F and T7 RNAP core fragment and promotes red light output
insulator BydvJ BBa_K2598010 ribozyme-based insulator
insulator RiboJ BBa_K2598014 ribozyme-based insulator
coding T7 BBa_K2598000 Non-active T7 RNAP core fragment being able to combine to being able to combine to sigma fragment to form a full-functional RNA polymerase
coding CcaSR BBa_K2598005 This part is a green-light sensor based on the membrane-associated histidine kinase CcaS and its response regulator CcaR, which can be switched on by green (535 nm) light, inducing the promoter PcpcG2-172 (BBa_K592003) and off by far-red (672 nm) light. So it can be used as a green light sensor
coding CGG BBa_K2598011 a T7 RNAP sigma fragment with DNA-binding domain that can be expressed when green light sensors are activated and be able to combine to non-active T7 RNAP core fragment (BBa_K2598001) to form a full-functional RNA polymerase and direct it to specific promoter PCGG(BBa_K2598023)
coding T3 BBa_K2598015 a T7 RNAP sigma fragment with DNA-binding domain that can be expressed when blue light sensors are activated and be able to combine to non-active T7 RNAP core fragment (BBa_K2598001) to form a full-functional RNA polymerase and direct it to specific promoter PT3(BBa_K2598026)
coding bFMO BBa_K2598027 bFMO is a bacterial flavin-containing monooxygenase that can catalyse tryptophan and render it blue
coding rain BBa_K2598058 encoding GDS, a enzyme catalyzing the conversion of Farnesyl diphosphate to Geosmin that has rain smell
coding mint BBa_K2598059 encoding BSMT1, a enzyme catalyzing the conversion of benzoic acid to methyl benzoate that has flower smell
coding lemon BBa_K2598060 encoding Limonene synthase 1, an enzyme catalyzing the conversion of Farnesyl diphosphate to (+)-Limonene that has lemon smell
teminator ECK120033737 BBa_K2598002 a strong teminator
teminator L3S2P11 BBa_K2598004 a strong teminator
teminator L3S2P55 BBa_K2598008 a strong teminator
teminator DT25 BBa_K2598012 a strong teminator
teminator L3S1P22 BBa_K2598017 a strong teminator
teminator DT11 BBa_K2598020 a strong teminator
teminator T7 BBa_K2598024 a strong teminator
teminator DT5 BBa_K2598028 a strong teminator
teminator DT16 BBa_K2598031 a strong teminator
teminator L3S3P11 BBa_K2598054 a strong teminator