Team:Edinburgh OG/Attributions
PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens
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Project
Project Background
Project Design
PHA Synthesis
Downstream Processing
Scaling Up
Model
Glucose Toggle Switch
InterLab Study
Parts List
Improve
Demonstrate
Practices
Integrated Human Practices
Education and Public Engagement
Life Cycle Assessment
Collaborations
Safety
Notebook
Weekly Journal
Protocol
Team
Acknowledgements
Attributions
Medal
Attributions
We are tremendously grateful to everyone who has kindly helped and guided us throughout our project. Your time, patience and effort is truly appreciated!
Team Valeris.ED
Cheewin (Big) Kittikunapong
: Team Leader, Crowdfunding, Integrated Human Practices Team, in silico modelling of project
Jackson DeKloe
: Team Leader, Integrated Human Practices Team, InterLab study, Glucose toggle switch development project
Lo Lok In (Owen)
: Jamboree trip coordinator, Integrated Human Practices Team, Education and Public Engagement, PHA production with Secretion system project
Ivonne Robledo
: Integrated Human Practices Team, Collaborated with Westminster iGEM team, Life cycle assessment project
Nabila Akhyar
: Logo Designer, Integrated Human Practices Team, Wiki Manager, Life cycle assessment project
Ming Zheng
: Wiki team, PHA production with
bktB
gene project
Siqi Wang
: Wiki team, Integrated Human Practices Team, PHA production with
sucCD
gene project
Craig Gilmour
: Collaborated with Iowa team, PHA production with SBM operon project
Qihui Lian
: PHA production with
phaR
gene-based autoregulation project
Peiyu Lu (Pepper)
: Integrated Human Practices Team, PHA operon project
Supervisors and Advisors
Dr Louise Horsfall
: Primary Investigator, supervised us on project planning, integrated human practices and academic work, provided her lab after dissertation submission for our project
Holly Robertson-Dick
: Secondary Investigator, supervised our iGEM work and advised us on iGEM requirements and fundraising
Dr Annegret Honsbein
: helped the team with troubleshooting and provided advice on planning experiments
Dr Heather Barker
: supervised our lab work and integrated human practices
Michael McDonald
: supervised our lab work
Dr Elise Cachat
: advised us on project planning and academic work
Dr David Clarke
: advised us on project planning and technical support
Project Support
Dr David Hills and Dr Elena Sugrue
: looked after us in the lab
Dr Leonardo Rios Solis
: advised us on simulation using SuperPro Designer
Dr Camilla Thomson
: advised us on life cycle assessment using SimaPro
Dr Ramon Grima
: guided us on dynamic modelling approach
Daniel Xiang, Stanford-Brown iGEM 2015
: provided dynamic approach model adapted in this project
Dr Martin Waterfall, Head of Flow Cytometry, Institute of Immunology & Infection Research at the University of Edinburgh
: taught us the use of the flow cytometer machine and showed how the work is conducted using flow cytometry measurement for our InterLab study
Edinburgh UG 2018 Team
: collaborated with us in the InterLab study
Westminster 2018 Team
: collaborated with us in the Life Cycle Assessment
Iowa UG 2018 Team
: collaborated with us in the detection of propionyl Co-A by their biosensor
Prof Paul Michels
: advised us on metabolic pathway
Dr Louise Horsfall Lab
: provided lab space and guidance
William Bothfeld, Northwestern University
: provided us with LacI(knock out) MG1655
E. coli
, pKDL071 plasmid, pKDL071-TaraF plasmid, and pKDL071-TaraF-phaECAB plasmid
Alan Taylor
: provided us access to Gas Chromatography and Mass Spectrophotometry, and advised us on the methodology and testings
Alan Isbister, John Hopkins, and Kirsten Affleck
: provided access to PCR machines and other equipment including flasks, shaker space, chemicals
Susan Forbes and Vicki Brown
: provided access to shaker space and drying oven
Prof Susan Rosser Lab
: provided access to chemicals and enzymes as well as shaker space
Dr Tessa Moses
: helped with gas chromatography inquiry
Jessica Birt
: advised on stoichiometric modelling and provided Benchling introduction
Christine Merrick and James Bryson
: helped with the competent cells
Felipe Aguilera Millacura
: helped with developing Sudan Black staining as an alternative strategy to confirm the production of PHA
Graham Nicholson and David Sneddon
: helped us to buy chemicals and anything we ran out of
Margaret Martin and her team
: helped to prepare medium and autoclaved flasks
Dr Jane Paget and Dr Hille Tekotte, Edinburgh Genome Foundry
: helped with cloning design, plate reader and quantitative measurement of PHA production
Dr David Kelly
: provided fluorescent microscopy for Nile red staining test
Ian Eggington and Miguel Cueva
: helped with cloning and primers design
Prof Mark Blaxter and Edinburgh Genomics
: provided us with ~800 GBP worth of free sequencing
Radhian Ferrel Armansyah
: helped with the wiki design
Dr Lorraine Kerr, Commercial Relations Manager
: provided information and contacts for Integrated Human Practices
MSc students in Tools of Synthetic Biology class 2017/2018
: provided valuable input that directed our project
Dr James Hallinan, Business Development Manager Synthetic Biology; Dr James Brown, Principal Scientist; and Dr Steve Thomas, Senior Consultant of Cambridge Consultant
: provided information
Dr Adrian Higson, Director and Lead Consultant for Biobased Products of The National Non-Food Crops Centre (NNFCC)
:
Morag Garden, Head of Sustainability and Innovation of Scotch Whisky Association
:
Dr Guoqiang Chen, Tsinghua University and BLUEPHA
:
Jin Yin, Technique Manager of BLUEPHA
:
Huang Jianfeng, Synthetic Resin Sales Division Manager of China Sinopec Guangzhou Branch
:
Paul Tan, Officer of China Sinopec Guangzhou Branch
:
Liu Hui, Manager of Tianan Biologic Material Co. Ltd.
:
Everyone who had generously supported us on our crowdfunding page