Team:UNSW Australia

ASSEMBLASE

Covalently Co-localising Enzymes in a Modular System

The diffusion of reaction intermediates limits the efficiency of many industrial biocatalytic pathways. The UNSW iGEM team has designed the Assemblase self-assembling scaffold system to counter this by increasing the rate and product yield of such reactions.

The scaffold specifically and covalently co-localises enzymes in a modular system. As a result, substrate can be channelled between enzymes at a much more efficient rate, thanks to the increased concentration of metabolic intermediates in the immediate surroundings of the enzymes.

The scaffold's modularity also means that it can be easily adapted for use in a range of pathways important in industry, bioremediation, and pharmaceutical synthesis. Proof of principle was established using the indole-acetic-acid biosynthesis pathway.

The Assemblase scaffold is a heterohexamer, composed of two alpha prefoldin and four beta prefoldin subunits. These subunits have been fused to Spy and Snoop Catchers, which covalently bind to Spy and Snoop Tags fused to enzymes of interest.

The scaffold design has many advantages. Firstly, prefoldin is highly thermostable and chemically resistant, being a protein derived from thermophilic archaea. This permits our scaffold to be used at high temperatures, allowing for increased kinetic energy in our system and therefore an increased rate of catalysis. Secondly, the use of Spy and Snoop Tag/Catcher systems gives our scaffold modularity, but with low cross-reactivity because of the specificity of the irreversible bonds. It also only requires small tags to be fused onto enzymes entering the system, and these are unlikely to interfere with enzyme functionality.

The Assemblase scaffold is therefore an incredibly useful foundational tool that may be adapted to co-localise a variety of enzymes for substrate channelling, which could have positive implications for a range of potential applications.