Demonstrate
Figure 1. Six 50 mL cultures of phaCB/Bktb in M9 media and 1% glucose were set up with three containing Pot ale and the other three containing water. Colonies were set up to shake at 37 oC overnight for 64 hours.
Figure 2. OD600 of cells grown in M9 media and 1% glucose with and without pot ale. Cells grown in pot ale and without pot ale similar growth curves.
Figure 3. Dry cell weight vs mass of plastic extracted of phaCB-Bktb cells grown in M9 media and 1% glucose with and without pot ale.
Figure 4. Melting temperature ranges of extracted plastic. Cultures grown on pot ale have consistant melting temperature ranges.