Team:BNDS CHINA/Attributions
The competition was organized at Beijing National Day school by Dr. Chun Zhang, Ms. Ruoting Tao, Zhuoyi Yang, and Xilin Yang. Yuchen Yang, Guanghua Hao, Zitian Ye, Jingxu Yan, Yinting Huang, Ziqi Zhao, Siying Peng, Kaiwen Dou, Yukun Xi, Zishi Wu, Yuezhang Zhou, Yuning Yang, and Zihan Zhang were recruited in December 2017.
Before determining our team project, we had rounds of discussion and every team member devoted in reading papers and sharing new ideas. As the team leader, Zhuoyi Yang and Xilin Yang organized lab skills training in winter holiday in Peking University with the help of Cheng Li, Hong Chen, Jianhui Qiu, Songyuan Zhang, Ke Bai, Chu Chen Wang, and Xiaoyu Yang from previous Peking iGEM teams. There are many institutes and people that helped us overcome many obstacles. The National Institute of Metrology provided many supports. The Laboratory of Biological Analysis and Cell Technologies，Division of Medical and Biological Measurement shared us their advanced laboratory facilities and equipment for us to conduct most of our experiments from August to September. Without their help, we could not finish our project. The Division of Chemical Metrology and Analytical Science lent us the HPLC/MS to measure the concentration of C4-HSL and constant temperature shaker to culture bacteria. Special thanks to Prof. Wang Jing, Dr. Gao Yunhua, Prof. Lu Xiaohua, Dr. Wang Jiayi, Prof. Wu Liqing, Dr. Dong Lianhua, Ms. Tang Hua and Dr. Song Dewei for their professional and unreserved help. The Laboratory of Structural Biology of Tsinghua University helped us with our induction, ultrasound bacteria lysis, protein purification, SDS-PAGE and most of the protein-related process. The National Center for Nanoscience and Technology shared us the Scanning Electron Microscope. Especially Prof. Chen Chunying and Prof. Piao Lingyu gave us many instructions to observe the antimicrobial ability of AMPs on Aeromonas Hydrophily. The Institute of Animal Quarantine Quarantine, Chinese Academy of Inspection and Inspection and Quarantine Technical Center of Beijing Customs shared us their P2 laboratory to conduct experiments with A.hydrophila, a biosafety level II bacteria. Mr. Yihao Zhang from School of Physics, Peking University Ms. Shuyu Shi and Mr. Jianhui Qiu from Medical School, Peking University gave us many useful pieces of advice on project and experiment design. Mr. Yihao Zhang kindly lent us the plate reader and taught us how to design RBS with desired strength in salislab.net. Prof. Li Xincang from East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences replied our email and solved our troubles on the experimental observation of the antimicrobial ability of AMP. Dr. Zhang Chun, Dr. Ruoting Tao as our PIs, Mr. Li Cheng as our instructor, Miss Hu Zhongxiu and Miss Wang Yantong as our advisers, gave us many suggestions on project design, experiment conduction, academic writing, and presentation. In addition, Dr. Xiangmei Dou from our school’s national department helped us to set up the synthetic biology lecture in her advanced biotechnology class. Mr. Wu Chunlei for instructing HTML and wiki design. Attributions
Our Roles in The Team
Name Wiki & Poster Human Practices & Collaboration Experiments Presentation Model Zishi Wu Experiments; Wiki design and composing; Drawing pictures; Poster design Red Window Expo; Meeting with UCAS; Team uniform design Induction of A.hydrophila sensors; Testing of AMPs; Interlab; Testing of QQ devices Quorum quenching ——— Yuchen Yang Experiments Red Window Expo Cloning of A. hydrophila sensor; Interlab; C4-HSL concentration measurement ——— ——— Guanghua Hao ——— Red Window Expo; Fish farming industry visit; Pamphlet making after fish trading market visit Interlab; Protein extraction for AMPs ——— ——— Zhuoyi Yang Demonstration; Collaboration; Design; Parts; Attributions Fish farming industry visit Winter holiday experiment training; Designing of experiment and devices; Interlab; Cloning; Testing of AMPs, A.hydrophila sensors, and QQ devices Collaborations & Special thanks Find the idea of model Zitian Ye Human Practice; Experiments Red Window Expo; Fish farming industry visit; Fish trading market visit Cloning; Testing of AMPs, A.hydrophila sensors, and QQ devices A. hydrophila sensor; Model ——— Xilin Yang Demonstration; Design; Experiments; poster design Fish farming industry visit Winter holiday experiment training; Designing of experiment and devices; Interlab; Cloning; Testing of AMPs, A.hydrophila sensors, and QQ devices Provide guidelines ——— Yukun Xi Human Practices Red Window Expo; Public lecture Cloning; Testing of AMPs ——— ——— Jingxu Yan Drawing pictures; Human practices Red Window Expo; Public lecture; Fish trading market visit Cloning; Testing of AMPs Introduction ——— Yinting Huang Human practices Red Window Expo; Public lecture; Team uniform design; Meeting with UCAS Cloning Human Practices ——— Ziqi Zhao Description Red Window Expo; Fish farming industry visit Testing of AMPs; Cloning AMPs ——— Siying Peng Human practices Red Window Expo; Fish trading market visit; meeting with UCAS Testing of AMPs; Cloning ——— ——— Yuezhang Zhou Description Red Window Expo; Team logo design Interlab; Cloning ——— ——— Kaiwen Dou Wiki design Red Window Expo; Public lecture Cloning ——— ——— Yuning Yang Model ——— A.hydrophila sensors ——— Model Zihan Zhang Model ——— A.hydrophila sensors ——— Model Special Thanks