Advanced CcaS with PSA domain knocked out with an opposite response to green/red light.
CcaS is a membrane protein functioning in CcaS/CcaR two-component system in response to green/red light. Under green light, the system and activate the transcription and expression of the specific genes downstream a proper promoter named PcpcG2, while the expression of the genes can be repressed when exposed to red light. This year, we were inspired by Professor Sode’ work of making a modification of CcaS’s structure and functions. And we deleted 858 base pairs in gene coding for CcaS, which means we removed the PSA domain in CcaS protein. When we characterized this new part, we surprised to find that the new CcaS had an opposite response to green/red light, compared to the original one. 放几张表征的图，然后附上part的链接http://parts.igem.org/Part:BBa_K2627000