Difference between revisions of "Team:Montpellier/L jensenii"
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<p>This toolbox contains all kind of information that can be useful for working with <i>Lactobacillus jensenii</i>. First, we provide culture conditions that we used and were proven to be working. Second, it contains a transformation protocol that we optimized and found to be the most efficient of all those we tested. Finally, the toolbox contains all DNA sequences (vectors and biobricks parts including <i>L. jensenii</i> specific promoters) that we used in <i>L. jensenii</i> for our project. We hope that this toolbox will facilitate the use of <i>L. jensenii</i> by other iGEM and research teams. We plan to extend the toolbox in the future by providing the community with a physical distribution of well-characterized vectors and regulatory elements.</p> | <p>This toolbox contains all kind of information that can be useful for working with <i>Lactobacillus jensenii</i>. First, we provide culture conditions that we used and were proven to be working. Second, it contains a transformation protocol that we optimized and found to be the most efficient of all those we tested. Finally, the toolbox contains all DNA sequences (vectors and biobricks parts including <i>L. jensenii</i> specific promoters) that we used in <i>L. jensenii</i> for our project. We hope that this toolbox will facilitate the use of <i>L. jensenii</i> by other iGEM and research teams. We plan to extend the toolbox in the future by providing the community with a physical distribution of well-characterized vectors and regulatory elements.</p> | ||
| − | <h2>How to grow <i>Lactobacillus jensenii</i>?</h2> | + | <h2>How to grow <i>Lactobacillus jensenii</i>?</h2><hr/> |
<p>For our project we used the following strain and culture medium :</p> | <p>For our project we used the following strain and culture medium :</p> | ||
| Line 20: | Line 20: | ||
<h4>Bacteria</h4> | <h4>Bacteria</h4> | ||
| − | <a class="lien" href="http://www.lgcstandards-atcc.org/products/all/25258.aspx?geo_country=fr" target="_blank"><i>Lactobacillus jensenii</i> Gasser et al. (ATCC® 25258™)</a> | + | <p><a class="lien" href="http://www.lgcstandards-atcc.org/products/all/25258.aspx?geo_country=fr" target="_blank"><i>Lactobacillus jensenii</i> Gasser et al. (ATCC® 25258™)</a></p> |
<h4>Culture</h4> | <h4>Culture</h4> | ||
| − | <a class="lien" href="https://www.fishersci.fr/shop/products/bd-difco-dehydrated-culture-media-lactobacilli-mrs-broth-3/11713553#?keyword=BD+288130&change_lang=true" target="_blank">BD Difco™ <i>Lactobacilli</i> MRS Broth</a> | + | <p><a class="lien" href="https://www.fishersci.fr/shop/products/bd-difco-dehydrated-culture-media-lactobacilli-mrs-broth-3/11713553#?keyword=BD+288130&change_lang=true" target="_blank">BD Difco™ <i>Lactobacilli</i> MRS Broth</a></p> |
<p>We used this broth for both our liquid and solid culture media. The liquid medium was used to do the solid medium by adding agar to it. We tested different concentrations of Agar to measure which one was the best.</p> | <p>We used this broth for both our liquid and solid culture media. The liquid medium was used to do the solid medium by adding agar to it. We tested different concentrations of Agar to measure which one was the best.</p> | ||
Revision as of 14:41, 14 October 2018
How to work with Lactobacillus jensenii?
This toolbox contains all kind of information that can be useful for working with Lactobacillus jensenii. First, we provide culture conditions that we used and were proven to be working. Second, it contains a transformation protocol that we optimized and found to be the most efficient of all those we tested. Finally, the toolbox contains all DNA sequences (vectors and biobricks parts including L. jensenii specific promoters) that we used in L. jensenii for our project. We hope that this toolbox will facilitate the use of L. jensenii by other iGEM and research teams. We plan to extend the toolbox in the future by providing the community with a physical distribution of well-characterized vectors and regulatory elements.
How to grow Lactobacillus jensenii?
For our project we used the following strain and culture medium :
Bacteria
Lactobacillus jensenii Gasser et al. (ATCC® 25258™)
Culture
BD Difco™ Lactobacilli MRS Broth
We used this broth for both our liquid and solid culture media. The liquid medium was used to do the solid medium by adding agar to it. We tested different concentrations of Agar to measure which one was the best.