Difference between revisions of "Team:SDU-CHINA/Attributions"

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{{SDU-CHINA}}
 
 
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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        width: 100%;
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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        height: 100%;
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
 
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<h1>Attributions</h1>
 
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<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
 
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</p>
 
  
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<h3> Bronze Medal Criterion #3</h3>
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#footer { height: 180px;  color: white; }
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<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
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#sponsors {
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<br><br>
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    text-align: center;
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Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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<div class="column third_size">
 
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<h3> What should this page contain?</h3>
 
  
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<ul>
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#section {clear: both;
−
<li>Clearly state what the team accomplished</li>
+
width:100%;
−
<li>General Support</li>
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margin-top:-20px;
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<li>Project support and advice</li>
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padding: 133px auto 0 auto;
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<li>Fundraising help and advice</li>
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overflow: hidden;
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<li>Lab support</li>
+
background-color:white !important;}
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<li>Difficult technique support</li>
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−
<li>Project advisor support</li>
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−
<li>Wiki support</li>
+
−
<li>Presentation coaching</li>
+
−
<li>Human Practices support</li>
+
−
<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
+
−
</ul>
+
−
</div>
+
  
−
<div class="column third_size">
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#main-title {
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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font-size: 45px;
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<ul>
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color: #4b5ea4;
−
<li>Does your institution teach an iGEM or synthetic biology course?</li>
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font-family: sans-serif;
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<li>When did you start this course?</li>
+
text-align: center;
−
<li>Are the syllabus and course materials freely available online?</li>
+
font-weight: lighter;
−
<li>When did you start your brainstorming?</li>
+
margin: 0;
−
<li>When did you start in the lab?</li>
+
padding: 30px 0px 0px 0px;
−
<li>When did you start working on  your project?</li>
+
margin: 0px 67px 0px 65px;
−
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−
<div class="column third_size">
 
−
<div class="highlight decoration_A_full">
 
  
−
<h3>Inspiration</h3>
+
p {
−
<p>Take a look at what other teams have done:</p>
+
    text-align: justify;
−
<ul>
+
    text-justify: inter-word;
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
+
    font-family: 'Calibri', sans-serif;
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
+
}
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
+
p {
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
+
    font-family: 'Open Sans', sans-serif;
−
</ul>
+
    font-weight: 400 !important;
−
</div>
+
    font-size: 18px !important;
−
</div>
+
    line-height: 1.5;
 +
    margin-bottom: 20px;
 +
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 +
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 +
    font-family: 'Josefin Sans', sans-serif !important;
 +
    font-size: 30px !important;
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 +
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 +
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 +
    display: -moz-inline-block;/* Firefox */
 +
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 +
         
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−
<div class="clear extra_space"></div>
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 +
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 +
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 +
    left:0px;
 +
clear:both;
 +
z-index: 3;
  
−
<div class="column two_thirds_size">
+
}
−
<h3> Why is this page needed? </h3>
+
#footer{
−
<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
+
    width: 100%;
−
<p>
+
    height:100px;  /* footer的高度一定要是固定值*/
−
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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 +
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 +
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 +
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 +
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 +
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 +
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 +
 
 +
 
 +
   
 +
</head>
 +
<body>
 +
 
 +
<div class="body">
 +
  <div id="header">
 +
  <!--LOGO LINK--->
 +
  <div id="logo"><a href="https://2018.igem.org/Team:SDU-CHINA"><img src="https://static.igem.org/mediawiki/2018/7/72/T--SDU-China--duihui.jpg" alt="LOGO" /></a></div>
 +
  <div id="menu">
 +
    <ul>
 +
            <li>
 +
                <a href="#">PROJECT &#9662;</a>
 +
                <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Description">Description</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Design">Design</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Results">Results</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Demonstrate">Demonstrate</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
 +
                <a href="#">WETLAB &#9662;</a>
 +
                <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Experiments">Protocols</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Notebook">Notebook</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Safety">Safety</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
 +
                <a href="#">DRYLAB &#9662;</a>
 +
                <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Model">Model</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Hardware">Hardware</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
 +
                <a href="#">TEAM &#9662;</a>
 +
                <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Team">Members</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Attributions">Attributoins</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Collaborations">Collaborations</a></li>
 +
                </ul>
 +
            </li>
 +
            <!--links-->
 +
            <li><a href="https://2018.igem.org/Team:SDU-CHINA/InterLab">INTERLAB</a></li>
 +
            <li><a href="#">PARTS &#9662;</a>
 +
            <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Parts">Parts</a></li>
 +
 
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Improve">Improve</a></li>
 +
                   
 +
                </ul>
 +
                </li>
 +
            <li><a href="https://2018.igem.org/Team:SDU-CHINA/Human_Practices">HUMAN PRACTICES &#9662;</a>
 +
            <ul>
 +
                    <!--links-->
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Human_Practices#Silver">Silver</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Human_Practices#integrated">Gold & Integrated</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SDU-CHINA/Public_Engagement">Engagement</a></li>
 +
                </ul>
 +
                </li>
 +
        </ul>
 +
  </div>
 
</div>
 
</div>
  
−
<div class="column third_size">
+
 
−
<div class="highlight decoration_B_full">
+
  <div class="conteudo">
−
<h3> Can we base our project on a previous one? </h3>
+
    <h1 id="main-title"> Attributions</h1>
−
<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
+
  <div class="paragraphs">
−
</div>
+
 
 +
 
 +
        <h4>Project</h4> <br>
 +
    <p><b>Ideal of the project:</b> <br>
 +
The project Metabolight was put forward after several rounds of brainstorm meetings in which all the team participated. And we discussed the feasibility with our PI, Professor Liang. <br><br>
 +
 
 +
<b>Molecular Cloning:</b><br>
 +
Design and construction of part CcaS# series (#3, #4, #10, #11): Yize Hao, Liyuan Xi, Zhaoqin Zhang and Junyang Chen.  <br>
 +
Design and construction of part opto-T7RNAP: Zijian Pan, Zilong Geng. <br>
 +
Design and construction of part pT7-crRNA: Zilong Geng, Jiao Jin, Yumin Meng, Zijian Pan.<br>
 +
Design and construction of pT7-sfGFP: Zijian Pan Zilong Geng.  <br>
 +
Design and construction of pT7-PHB: Zijian Pan <br>
 +
Design and construction of plasmid araBAD-crRNA: Liyuan Xi  <br>
 +
Design and construction of part PcpcG2-crRNA: Yize Hao,Liyuan Xi,  Zhaoqin Zhang and Junyang Chen <br>
 +
Design and construction of part PcpcG2-PHB: Liyuan Xi, Zijian Pan, and Yize Hao. <br>
 +
Design and construction of part phbcab: Our PI. <br><br>
 +
 
 +
<b>Characterization：</b><br>
 +
The target site of crRNA was designed by Yizhao Chang and data of fluorescence intensity was provided by Yizhao Chang. <br>
 +
The characterization of crRNA was designed by Yizhao Chang and conducted by Jiao Jin and Yize Hao. Abs600 was measured by Jiao Jin and cells were inoculated by Yize Hao. <br>
 +
The characterization of CcaS/CcaR was conducted by Yize Hao, Liyuan Xi, Junyang Chen, Jiao Jin, Zijian Pan according to the protocol from the reference. Fluorescence intensity was measured by Liyuan Xi and Junyang Chen, measurement of Abs600 was done by Jiao Jin and Zijian Pan. <br>
 +
The characterization of opto-T7RNAP was conducted by Zilong Geng, Jiao Jin, Zijian Pan and Yumin Meng according to the protocol from the reference. SDS-PAGE was done by Zilong Geng <br>
 +
      <br><br>
 +
 
 +
<b>Fermentation:</b> <br>
 +
The Fermentation was designed and conducted by Yize Hao, Zijian Pan, Jiao Jin, and Liyuan Xi <br><br>
 +
 
 +
<b>Hardware:</b> <br>
 +
The light-device 1.0 was designed and made by Weiguang Wang. <br><br>
 +
 
 +
<b>Modeling:</b> <br>
 +
The modeling work was performed by Jizhong Zhang. <br><br>
 +
 
 +
<b>Wiki&art:</b> <br>
 +
Wiki and art are designed and constructed by Yize Hao and Jizhong Zhang. The text was edited by the rest of the Team and polished by Zedao Liu. <br><br>
 +
 
 +
<b>Standardization</b> <br>
 +
Standardization was conducted by Zhaoqin Zhang, Jiao Jin, Yumin Meng, Zijian Pan Liyuan Xi.  <br><br>
 +
 
 +
<b>Interlab</b> <br>
 +
Interlab was conducted by Junyang Chen. <br><br>
 +
 
 +
<b>Human Practices</b> <br>
 +
Human practice was conducted by Jiao Jin, Yize Hao, Zhaoqin Zhang, Yumin Meng and Zilong Geng. <br><br>
 +
 
 +
<b>Funding </b> <br>
 +
Initial founds of the project was raised by Zilong Geng from SDU and subsequently by Qingsheng Qi (Profeesor at SDU，qiqingsheng@sdu.edu.cn) and Quanfeng Liang (Profeesor at SDU，liangquanfeng@sdu.edu.cn).<br><br><br></p>
 +
 
 +
 
 +
<h4>Supervision</h4> <br>
 +
<p><b>Wet lab</b>  <br>
 +
Wet lab and experiments are mainly supervised by Wei Jiang (PhD student) and Chang. And the bio-safety was supervised by Quanfeng Liang. <br><br>
 +
 
 +
<b>Dry Lab</b><br>
 +
The modeling and hardware are supervised by Huaiwei Liu (associate professor at SDU，liuhuaiwei@sdu.edu.cn). <br><br>
 +
 
 +
<b>Human Practices</b> <br>
 +
Human practice was supervised by Qanfeng Liang and Qingsheng Qi. <br></p><br>
 +
 
 +
 
 +
<h4>Acknowledgements</h4> <br>
 +
<p>Our PI Prof. <b>Quanfeng Liang</b> and Prof. <b>Qingsheng Qi</b>, offered us the lab and equipment, along with constructive advice over our project and long term technical consulting support through the whole procedure.
 +
<b>Wei Jiang</b> (Phd student), <b>Fei Gu</b> (PhD student), gave us molecular cloning training, and modified the fermentations.
 +
<b>Yizhao Chang</b> (research assistant), provided us with the parts he constructed nd modified the fermentations.<br><br>
 +
 
 +
<b>2017 OUC-China iGEM</b>, gave us practical suggestions at the very beginning of the team.<br><br>
 +
 
 +
<b>2018 BIT-China iGEM</b>, gave us practical advice on the hardware.<br><br>
 +
 
 +
<b>Prof Yi Yang</b>, from East China University of Science and Technology，gave us advise on the hardware and the potential of light-controlled systems.<br><br>
 +
 
 +
 
 +
 
 +
Our mentor <b>Junhao Lu</b>, from University of Alberta, gave us advice on the experiments and poster.<br><br>
 +
 
 +
Our adviser <b>Xianghui Zhao</b>，gave us advice on wiki. <br><br>
 +
Tianyuan Liu, from Shandong University, helped us with the poster. Also thanks for Gang Li designing logo for this team and Yuning Zheng paiting some attractive pictures for our wiki!<br><br>
 +
Weifang Continent Biotechnology Co. Ltd. provided us with the access to factory practice. <br><br>
 +
<a href="www.taobao.com"><b>Taobao</b></a>, provided us with hardware we used in the project.<br><br><br><br>
 +
</p>
 +
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Latest revision as of 01:00, 8 December 2018

Attributions

Project


Ideal of the project:
The project Metabolight was put forward after several rounds of brainstorm meetings in which all the team participated. And we discussed the feasibility with our PI, Professor Liang.

Molecular Cloning:
Design and construction of part CcaS# series (#3, #4, #10, #11): Yize Hao, Liyuan Xi, Zhaoqin Zhang and Junyang Chen.
Design and construction of part opto-T7RNAP: Zijian Pan, Zilong Geng.
Design and construction of part pT7-crRNA: Zilong Geng, Jiao Jin, Yumin Meng, Zijian Pan.
Design and construction of pT7-sfGFP: Zijian Pan Zilong Geng.
Design and construction of pT7-PHB: Zijian Pan
Design and construction of plasmid araBAD-crRNA: Liyuan Xi
Design and construction of part PcpcG2-crRNA: Yize Hao,Liyuan Xi, Zhaoqin Zhang and Junyang Chen
Design and construction of part PcpcG2-PHB: Liyuan Xi, Zijian Pan, and Yize Hao.
Design and construction of part phbcab: Our PI.

Characterization：
The target site of crRNA was designed by Yizhao Chang and data of fluorescence intensity was provided by Yizhao Chang.
The characterization of crRNA was designed by Yizhao Chang and conducted by Jiao Jin and Yize Hao. Abs600 was measured by Jiao Jin and cells were inoculated by Yize Hao.
The characterization of CcaS/CcaR was conducted by Yize Hao, Liyuan Xi, Junyang Chen, Jiao Jin, Zijian Pan according to the protocol from the reference. Fluorescence intensity was measured by Liyuan Xi and Junyang Chen, measurement of Abs600 was done by Jiao Jin and Zijian Pan.
The characterization of opto-T7RNAP was conducted by Zilong Geng, Jiao Jin, Zijian Pan and Yumin Meng according to the protocol from the reference. SDS-PAGE was done by Zilong Geng


Fermentation:
The Fermentation was designed and conducted by Yize Hao, Zijian Pan, Jiao Jin, and Liyuan Xi

Hardware:
The light-device 1.0 was designed and made by Weiguang Wang.

Modeling:
The modeling work was performed by Jizhong Zhang.

Wiki&art:
Wiki and art are designed and constructed by Yize Hao and Jizhong Zhang. The text was edited by the rest of the Team and polished by Zedao Liu.

Standardization
Standardization was conducted by Zhaoqin Zhang, Jiao Jin, Yumin Meng, Zijian Pan Liyuan Xi.

Interlab
Interlab was conducted by Junyang Chen.

Human Practices
Human practice was conducted by Jiao Jin, Yize Hao, Zhaoqin Zhang, Yumin Meng and Zilong Geng.

Funding
Initial founds of the project was raised by Zilong Geng from SDU and subsequently by Qingsheng Qi (Profeesor at SDU，qiqingsheng@sdu.edu.cn) and Quanfeng Liang (Profeesor at SDU，liangquanfeng@sdu.edu.cn).


Supervision


Wet lab
Wet lab and experiments are mainly supervised by Wei Jiang (PhD student) and Chang. And the bio-safety was supervised by Quanfeng Liang.

Dry Lab
The modeling and hardware are supervised by Huaiwei Liu (associate professor at SDU，liuhuaiwei@sdu.edu.cn).

Human Practices
Human practice was supervised by Qanfeng Liang and Qingsheng Qi.


Acknowledgements


Our PI Prof. Quanfeng Liang and Prof. Qingsheng Qi, offered us the lab and equipment, along with constructive advice over our project and long term technical consulting support through the whole procedure. Wei Jiang (Phd student), Fei Gu (PhD student), gave us molecular cloning training, and modified the fermentations. Yizhao Chang (research assistant), provided us with the parts he constructed nd modified the fermentations.

2017 OUC-China iGEM, gave us practical suggestions at the very beginning of the team.

2018 BIT-China iGEM, gave us practical advice on the hardware.

Prof Yi Yang, from East China University of Science and Technology，gave us advise on the hardware and the potential of light-controlled systems.

Our mentor Junhao Lu, from University of Alberta, gave us advice on the experiments and poster.

Our adviser Xianghui Zhao，gave us advice on wiki.

Tianyuan Liu, from Shandong University, helped us with the poster. Also thanks for Gang Li designing logo for this team and Yuning Zheng paiting some attractive pictures for our wiki!

Weifang Continent Biotechnology Co. Ltd. provided us with the access to factory practice.

Taobao, provided us with hardware we used in the project.