Difference between revisions of "Team:BNDS CHINA/Description"

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fig3:
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<p>Instead of using AhyR, however, we use RhlR as our transcriptional regulator. RhlR-C4-HSL complex will bind to the promoter region and activate the transcription of the downstream gene in our engineered E. coli.</p>
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<div align=center><img width="70%" src="https://static.igem.org/mediawiki/2018/2/20/T--BNDS_CHINA--tani.gif"/>
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  <p>Fig 3. Quorum Sensing of AhyRI and RhlRI system（就是第一个gif）</p></div>
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<p>Many pathogenesis-related factors of A. hydrophila, including expression of virulence factors (such as hemolysin, protease, S layer proteins, DNase, amylase), biofilm maturation, and the type II, III and VI secretion system, are reported under the regulation of quorum sensing in Aeromonas hydrophila. (Cao et al., 2012) In this project, we aim at cutting the QS pathway within A. hydrophila population to cure its infection on fish.</p>
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<p>To block QS within A. hydrophila, we engineer E. coli to make it expressing an autoinducer inactivation enzyme aiiA: after the transcriptional regulator RhlR is bound with C4-HSL in the environment, the complex activates the expression of aiiA which inactivate the surrounding C4-HSL to reduce QS within A. hydrophila population. aiiA is a lactonase which cleaves the homoserine lactone ring of molecule AHLs in a hydrolytic and reversible reaction.</p>
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<p>Fig 4. Inactivation of C4-HSL</p>
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<p>The aiiA we use is AHL-lactonase147-11516 from Bacillus thuringiensis strain 147-11516. It prefers AHLs with shorter acyl chains and thus is observed to have higher specificity with C4-HSL. As it has biological activity under alkaline pH conditions, it is effective in fish’s intestine. (Pedroza, Flórez, Ruiz & Orduz, 2013) We optimize the codons in the sequence BBa_C0160 as the second quorum quenching enzyme.</p>
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<div align=center><img width="70%" src="https://static.igem.org/mediawiki/2018/2/2c/T--BNDS_CHINA--description-fig5.gif"/>
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  <p>Fig 5. Quorum Quenching (第二个gif。。。）</p></div>
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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h3>What should this page contain?</h3>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
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<h3>References</h3>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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Revision as of 02:18, 16 October 2018

fig3: fig5:

Instead of using AhyR, however, we use RhlR as our transcriptional regulator. RhlR-C4-HSL complex will bind to the promoter region and activate the transcription of the downstream gene in our engineered E. coli.

Fig 3. Quorum Sensing of AhyRI and RhlRI system（就是第一个gif）

Many pathogenesis-related factors of A. hydrophila, including expression of virulence factors (such as hemolysin, protease, S layer proteins, DNase, amylase), biofilm maturation, and the type II, III and VI secretion system, are reported under the regulation of quorum sensing in Aeromonas hydrophila. (Cao et al., 2012) In this project, we aim at cutting the QS pathway within A. hydrophila population to cure its infection on fish.

To block QS within A. hydrophila, we engineer E. coli to make it expressing an autoinducer inactivation enzyme aiiA: after the transcriptional regulator RhlR is bound with C4-HSL in the environment, the complex activates the expression of aiiA which inactivate the surrounding C4-HSL to reduce QS within A. hydrophila population. aiiA is a lactonase which cleaves the homoserine lactone ring of molecule AHLs in a hydrolytic and reversible reaction.

Fig 4. Inactivation of C4-HSL

The aiiA we use is AHL-lactonase147-11516 from Bacillus thuringiensis strain 147-11516. It prefers AHLs with shorter acyl chains and thus is observed to have higher specificity with C4-HSL. As it has biological activity under alkaline pH conditions, it is effective in fish’s intestine. (Pedroza, Flórez, Ruiz & Orduz, 2013) We optimize the codons in the sequence BBa_C0160 as the second quorum quenching enzyme.

Fig 5. Quorum Quenching (第二个gif。。。）