Project

Ideal of the project:
The project Metabolight was put forward after several rounds of brainstorm meetings in which all the team participated. And we discussed the feasibility with our PI, Professor Liang.

Molecular Cloning:
Design and construction of part CcaS# series (#3, #4, #10, #11): Yize Hao, Liyuan Xi, Zhaoqin Zhang and Junyang Chen.
Design and construction of part opto-T7RNAP: Zijian Pan, Zilong Geng.
Design and construction of part pT7-crRNA: Zilong Geng, Jiao Jin, Yumin Meng, Zijian Pan.
Design and construction of pT7-sfGFP: Zijian Pan Zilong Geng.
Design and construction of pT7-PHB: Zijian Pan
Design and construction of plasmid araBAD-crRNA: Yizhao Chang (research assistant)
Design and construction of part PcpcG2-crRNA: Yize Hao,Liyuan Xi, Zhaoqin Zhang and Junyang Chen
Design and construction of part PcpcG2-PHB: Liyuan Xi, Zijian Pan, and Yize Hao.
Design and construction of part phbcab: Our PI.

Characterization：
The target site of crRNA was designed by Yizhao Chang and data of fluorescence intensity was provided by Yizhao Chang.
The characterization of crRNA was designed by Yizhao Chang and conducted by Jiao Jin and Yize Hao. Abs600 was measured by Jiao Jin and cells were inoculated by Yize Hao.
The characterization of CcaS/CcaR was conducted by Yize Hao, Liyuan Xi, Junyang Chen, Jiao Jin, Zijian Pan according to the protocol from the reference. Fluorescence intensity was measured by Liyuan Xi and Junyang Chen, measurement of Abs600 was done by Jiao Jin and Zijian Pan.
The characterization of opto-T7RNAP was conducted by Zilong Geng, Jiao Jin, Zijian Pan and Yumin Meng according to the protocol from the reference. SDS-PAGE was done by Zilong Geng


Fermentation:
The Fermentation was designed and conducted by Yize Hao, Zijian Pan, Jiao Jin, and Liyuan Xi

Hardware:
The light-device 1.0 was designed and made by Weiguang Wang.

Modeling:
The modeling work was performed by Jizhong Zhang.

Wiki&art:
Wiki and art are designed and constructed by Yize Hao and Jizhong Zhang. The text was edited by the rest of the Team and polished by Zedao Liu.

Standardization
Standardization was conducted by Zhaoqin Zhang, Jiao Jin, Yumin Meng, Zijian Pan Liyuan Xi.

Interlab
Interlab was conducted by Junyang Chen.

Human Practices
Human practice was conducted by Jiao Jin, Yize Hao, Zhaoqin Zhang, Yumin Meng and Zilong Geng.

Funding
Initial founds of the project was raised by Zilong Geng from SDU and subsequently by Qingsheng Qi (Profeesor at SDU，qiqingsheng@sdu.edu.cn) and Quanfeng Liang (Profeesor at SDU，liangquanfeng@sdu.edu.cn).

Supervision

Wet lab
Wet lab and experiments are mainly supervised by Wei Jiang (PhD student) and Chang. And the bio-safety was supervised by Quanfeng Liang.

Dry Lab
The modeling and hardware are supervised by Huaiwei Liu (associate professor at SDU，liuhuaiwei@sdu.edu.cn).

Human Practices
Human practice was supervised by Qanfeng Liang and Qingsheng Qi.

Acknowledgements

Our PI Prof. Quanfeng Liang and Prof. Qingsheng Qi, offered us the lab and equipment, along with constructive advice over our project and long term technical consulting support through the whole procedure. Wei Jiang (Phd student), Fei Gu (PhD student), gave us molecular cloning training, and modified the fermentations. Yizhao Chang (research assistant), provided us with the parts he constructed nd modified the fermentations.

2017 OUC-China iGEM, gave us practical suggestions at the very beginning of the team.

2018 BIT-China iGEM, gave us practical advice on the hardware.

Prof Yi Yang, from East China University of Science and Technology，gave us advise on the hardware and the potential of light-controlled systems.

Our mentor Junhao Lu, from University of Alberta, gave us advice on the experiments and poster.

Our adviser Xianghui Zhao，gave us advice on wiki.

Tianyuan Liu, from Shandong University, helped us with the poster.

Weifang Continent Biotechnology Co. Ltd. provided us with the access to factory practice.

Taobao, provided us with hardware we used in the project.