Team:Montpellier/L jensenii
How to work with Lactobacillus jensenii?
This toolbox contains all kind of information that can be useful for working with Lactobacillus jensenii. First, we provide culture conditions that we used and were proven to be working. Second, it contains a transformation protocol that we optimized and found to be the most efficient of all those we tested. Finally, the toolbox contains all DNA sequences (vectors and biobricks parts including L. jensenii specific promoters) that we used in L. jensenii for our project. We hope that this toolbox will facilitate the use of L. jensenii by other iGEM and research teams. We plan to extend the toolbox in the future by providing the community with a physical distribution of well-characterized vectors and regulatory elements.
How to grow Lactobacillus jensenii?
For our project we used the following strain and culture medium :
Bacteria
Lactobacillus jensenii Gasser et al. (ATCC® 25258™)
Culture
BD Difco™ Lactobacilli MRS Broth
We used this broth for both our liquid and solid culture media. The liquid medium was used to do the solid medium by adding agar to it. We tested different concentrations of Agar to measure which one was the best.
5g/L
7g/L
10g/L
12g/L
15g/L