Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.
Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.
Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.
|BBa_K2810000||Regulatory||WRKY intron for plant-targeted RNAi constructs||Geraint Parry||295bp|
|BBa_K2810001||Reporter||GUS reporter for plant expression||Geraint Parry||1817bp|
|BBa_K2810002||Regulatory||RTBV Vascular Specific Promotor||Geraint Parry||759bp|
|BBa_K2810003||RNA||Full sequence for Myzus persisae BCR3 secreted protein||Geraint Parry||257bp|
|BBa_K2810004||RNA||Complimentary sequence to Myzus persisae BCR3 secreted protein||Geraint Parry||257bp|
|BBa_K2810005||RNA||Coding sequence from 5' region of Myzus persisae BCR3 secreted protein||Geraint Parry||102bp|
|BBa_K2810006||RNA||Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein||Geraint Parry||102bp|
|BBa_K2810007||RNA||siRNA from 5' region of Myzus persicae SP3 secreted protein||Ryan Coates||291bp|
|BBa_K2810008||RNA||siRNA* from 5' region of Myzus persicae SP3 secreted protein||Ryan Coates||291bp|
|BBa_K2810009||Reporter||mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible||Ryan Coates||676bp|
|BBa_K2810010||Regulatory||Regulatory region derived from the ribitol-responsive operon||Ryan Coates||169bp|
|BBa_K2810011||Composite||mCherry under control of the bacterial promoter and terminato||Ryan Coates||858bp|