Team:Cardiff Wales/Parts

Parts

Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.

Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.

Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.





Name Type Description Designer Length
BBa_K2810000 Regulatory WRKY intron for plant-targeted RNAi constructs Geraint Parry 295bp
BBa_K2810001 Reporter GUS reporter for plant expression Geraint Parry 1817bp
BBa_K2810002 Regulatory RTBV Vascular Specific Promotor Geraint Parry 759bp
BBa_K2810003 RNA Full sequence for Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_K2810004 RNA Complimentary sequence to Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_K2810005 RNA Coding sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_K2810006 RNA Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_K2810007 RNA siRNA from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_K2810008 RNA siRNA* from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_K2810009 Reporter mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible Ryan Coates 676bp
BBa_K2810010 Regulatory Regulatory region derived from the ribitol-responsive operon Ryan Coates 169bp
BBa_K2810011 Composite mCherry under control of the bacterial promoter and terminato Ryan Coates 858bp