Parts
Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.
Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.
Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K2810000 | Regulatory | WRKY intron for plant-targeted RNAi constructs | Geraint Parry | 295bp |
BBa_K2810001 | Reporter | GUS reporter for plant expression | Geraint Parry | 1817bp |
BBa_K2810002 | Regulatory | RTBV Vascular Specific Promotor | Geraint Parry | 759bp |
BBa_K2810003 | RNA | Full sequence for Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
BBa_K2810004 | RNA | Complimentary sequence to Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
BBa_K2810005 | RNA | Coding sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
BBa_K2810006 | RNA | Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
BBa_K2810007 | RNA | siRNA from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
BBa_K2810008 | RNA | siRNA* from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
BBa_K2810009 | Reporter | mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible | Ryan Coates | 676bp |
BBa_K2810010 | Regulatory | Regulatory region derived from the ribitol-responsive operon | Ryan Coates | 169bp |
BBa_K2810011 | Composite | mCherry under control of the bacterial promoter and terminato | Ryan Coates | 858bp |