Best Composite Part Special Prize
For this award we nominate our part BBa_K2770002 because for that particular part we were able to show protein production via Western Blot, were able to purify it using an Äkta, showed in vitro protein activity and product yield using an HPLC. For further information on the particular results please visit the respective pages on our wiki and the registry. //Comment den ich gerne schreiben würde, aber vlt lieber lassen:// Actually, we could show the mentioned characterizations for all composite parts except the ICL1 expression cassette BBa_K2770007, but for our nominated part we did the experiments the most often with the same positive results and it is the first in our list.
Composite Parts
In the following table are all composite parts listed that we produced this year and submitted to the registry.
part number | part type | produced enzyme | production organism |
---|---|---|---|
BBa_K2770002 | Generator | YcdW - E. coli glyoxylate reductase | E. coli |
BBa_K2770003 | Generator | AceA - E. coli isocitrate lyase | E. coli |
BBa_K2770006 | Generator | Glyr1 - A. thaliana glyoxylate reductase 1 | S. cerevisae |
BBa_K2770007 | Generator | ICL1 - S. cerevisae isocitrate lyase 1 | S. cerevisae |
BBa_K2770010 | Generator | CHMO - A. calcoaceticus cyclohexanone monooxygenase | E. coli |
BBa_K2770011 | Generator | ADH - L. kefiri alcohol dehydrogenase | E. coli |
Additionally, here are the basic parts listed, which have been used for correct assembly of the composite parts. Therefore, none of these basic parts has been submitted to the registry by us. Some of them are designed by other teams as indicated in the table.
part number | part type | short description | source organism | designed by |
---|---|---|---|---|
BBa_K2770000 | cds | ycdW - glyoxylate reductase | E. coli | TU_Darmstadt 2018 |
BBa_K2770001 | cds | aceA - isocitrate lyase | E. coli | TU_Darmstadt 2018 |
BBa_K2770004 | cds | glyr1 - glyoxylate reductase | A. thaliana | TU_Darmstadt 2018 |
BBa_K2770005 | cds | icl1 - isocitrate lyase | S. cerevisae | TU_Darmstadt 2018 |
BBa_K2770008 | cds | chmo - cyclohexanone monooxygenase | A. calcoaceticus | TU_Darmstadt 2018 |
BBa_K2770009 | cds | adh - alcohol dehydrogenase | L. kefiri | TU_Darmstadt 2018 |
BBa_K2770012 | protein domain | GS-Linker GGSGG | - | TU_Darmstadt 2018 |
BBa_K2770013 | Affinity-Tag | 10x His-Tag | - | TU_Darmstadt 2018 |
BBa_K2770014 | Linker | SA Linker | - | TU_Darmstadt 2018 |
BBa_K2770015 | Affinity-Tag | Strep-Tag II | - | TU_Darmstadt 2018 |
BBa_K2770016 | DNA | AAA sequence for correct assembly | - | TU_Darmstadt 2018 |
BBa_J70593 | DNA | Startcodon | - | Knight Lab 2010 |
BBa_K2389005 | Restriction Site | Kpn1 restriction site | - | UAlberta 2017 |
BBa_KM31383 | Restriction Site | Nco1 restriction site | - | Instructors MIT 2007 |
BBa_G0000 | DNA | SpeI/XbaI Scar | - | iGEM 2007 |
BBa_M36117 | DNA | Stop-Codon | - | Stanford 2012 |
BBa_K921000 | Promotor | T7/Lac Promotor | T7 bacteriophage | Carnegie_Mellon 2012 |
BBa_B0034 | RBS | RBS | E. coli | Antiquity 2003 |