Team:TU Darmstadt/Composite Part
Best Composite Part Special Prize
For this award we nominate our part BBa_K2770002. This part is our favorite, as we were able to show functionality of all relevant steps, and could characterize our part accordingly. Transcription and translation of its encoded gene ycdW was shown by western blot. We could purify His-tagged YcdW using an Äkta, could prove YcdWs function by an in vitro glycolate production assay and were able to show product yield using HPLC. For detailed information please visit the respective pages on our wiki and the registry.
Composite Parts
Listed in the following table are all composite parts that we produced this year and submitted to the registry. The part we nominated for best composite part is BBa_K2770002, it is marked with a star in the table.
| part number | part type | produced enzyme | production organism |
|---|---|---|---|
| ✦ BBa_K2770002 | Generator | YcdW - E. coli glyoxylate reductase | E. coli |
| BBa_K2770003 | Generator | AceA - E. coli isocitrate lyase | E. coli |
| BBa_K2770006 | Generator | GLYR1 - A. thaliana glyoxylate reductase 1 | S. cerevisiae |
| BBa_K2770007 | Generator | ICL1 - S. cerevisiae isocitrate lyase 1 | S. cerevisiae |
| BBa_K2770010 | Generator | CHMO - A. calcoaceticus cyclohexanone monooxygenase | E. coli |
| BBa_K2770011 | Generator | ADH - L. kefiri alcohol dehydrogenase | E. coli |
Below we listed the basic parts we used for correct assembly of the above-mentioned composite parts. Therefore, none of these basic parts have been submitted to the registry by our team. Some of them have previously been designed by other iGEM teams, as indicated in the table . All sequences of parts from previous iGEM teams were taken form the part registry and our assembled composite parts have been synthesized by IDT. ✌
| part number | part type | short description | source organism | designed by |
|---|---|---|---|---|
| BBa_K2770000 | cds | ycdW - glyoxylate reductase | E. coli | TU_Darmstadt 2018 |
| BBa_K2770001 | cds | aceA - isocitrate lyase | E. coli | TU_Darmstadt 2018 |
| BBa_K2770004 | cds | GLYR1 - glyoxylate reductase | A. thaliana | TU_Darmstadt 2018 |
| BBa_K2770005 | cds | ICL1 - isocitrate lyase | S. cerevisiae | TU_Darmstadt 2018 |
| BBa_K2770008 | cds | chmo - cyclohexanone monooxygenase | A. calcoaceticus | TU_Darmstadt 2018 |
| BBa_K2770009 | cds | adh - alcohol dehydrogenase | L. kefiri | TU_Darmstadt 2018 |
| BBa_K2770012 | protein domain | GS-Linker GGSGG | - | TU_Darmstadt 2018 |
| BBa_K2770013 | Affinity-Tag | 10x His-Tag | - | TU_Darmstadt 2018 |
| BBa_K2770014 | Linker | SA Linker | - | TU_Darmstadt 2018 |
| BBa_K2770015 | Affinity-Tag | Strep-Tag II | - | TU_Darmstadt 2018 |
| BBa_K2770016 | DNA | AAA sequence for correct assembly | - | TU_Darmstadt 2018 |
| BBa_J70593 | DNA | Startcodon | - | Knight Lab 2010 |
| BBa_K2389005 | Restriction Site | Kpn1 restriction site | - | UAlberta 2017 |
| BBa_KM31383 | Restriction Site | Nco1 restriction site | - | Instructors MIT 2007 |
| BBa_G0000 | DNA | SpeI/XbaI Scar | - | iGEM 2007 |
| BBa_M36117 | DNA | Stop-Codon | - | Stanford 2012 |
| BBa_K921000 | Promotor | T7/Lac Promotor | T7 bacteriophage | Carnegie_Mellon 2012 |
| BBa_B0034 | RBS | RBS | E. coli | Antiquity 2003 |