Difference between revisions of "Team:Valencia UPV/Attributions"

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#content { margin-left:0px; margin-top:-30px; padding:0px; width:100%;}
  
<div class="column full_size judges-will-not-evaluate">
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</style>
<h3>★  ALERT! </h3>
+
 
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<head>
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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  <meta charset="utf-8">
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  <title>Stack Multipurpose HTML Template</title>
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  <meta name="viewport" content="width=device-width, initial-scale=1.0">
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  <meta name="description" content="Site Description Here">
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  <link href="https://fonts.googleapis.com/css?family=Open+Sans:200,300,400,400i,500,600,700%7CMerriweather:300,300i" rel="stylesheet">
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  <link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
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  <link href="https://fonts.googleapis.com/css?family=Lato:400,700,900" rel="stylesheet">
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  <link href="https://2018.igem.org/Template:Valencia_UPV/fontAwesome?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
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<body class="" style="background-color:#353535;">
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  <a id="start"></a>
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<div class="main-container">
  
  
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<!-------------------------------IMAGEN PRINCIPAL------------------------------------------------>
  
  
 +
<section style="
 +
; ; background-size: 100%;height: 44.2em;margin-top: 0em;padding-top: 1.1em;" class="height-90 parallax">
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  height: 100%;margin-top: 4em;">
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  <div id="logoPrinteria" class="item" style="
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    background-image: url(https://static.igem.org/mediawiki/2018/3/3b/T--Valencia_UPV--laboratorioAdrianUPV2018.jpeg);
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    height: 100%;
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    width: 100%;background-attachment: fixed;background-size: cover;">
 +
   
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    <div style="/* position: absolute; *//* top: 81%; *//* padding-left: 31em; */">
 +
      <a class="btn down inner-link active" href="#story" style="font-size: 82%;right: 50%;/* position: fixed; *//* bottom: 7%; */top: 85.2%;z-index: 99;background-color: white;position: absolute;border-radius: 80%;width: 3.8em;height: 3.8em;padding: 0;padding-top: 14px;">
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        <i class="stack-interface stack-down-open-big"></i>
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      </a>
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    </div>
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  </div>
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</div>
  
 +
</center>
  
 +
</div><div class="all-page-modals"></div><div class="all-page-modals"></div>
 +
<div class="all-page-modals"></div>
 +
</section></div>
  
  
<div class="column full_size">
+
<!----------------------EMPIEZA LA SECCIÓN DE DEBAJO DE LA IMAGEN ----------------------------->
<h1>Attributions</h1>
+
<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
+
</p>
+
  
<h3> Bronze Medal Criterion #3</h3>
 
<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
 
<br><br>
 
Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
 
</div>
 
  
 +
<section id="story" class="feature-large" style="padding-top: 6em; padding-bottom: 50px; outline: none;" data-scroll-id="story" tabindex="-1">
 +
<div class="container">
 +
  <div class="row">
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    <div class="col-md-3" style="
 +
      margin-left: 0em;
 +
      padding-right: 0px;
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      padding-left: 0.2em;
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      ">
 +
     
 +
      <!------------ÍNDICE LATERAL------------------>
  
<div class="clear extra_space"></div>
+
      <div class="tabs-container tabs--vertical page-navigator" style="position: -webkit-sticky;position: sticky;padding-bottom: 0px;margin-top: 11.4em;">
 +
        <h4 style="
 +
        padding-left: 1em;
 +
        ">Index</h4>
 +
        <ul class="lateral">
 +
          <li class="lateral" style="margin-bottom: 0px;padding-bottom: 1em;padding-top: initial;padding-left: 1em;/* color: #353535; */">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
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              /* padding-left: 0.5em; */
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              ">
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              <a class="lateral" style="
 +
                /* color: #ffffff; */
 +
                opacity: 1;
 +
                list-style-type: circle;
 +
              ">INTRODUCTION</a>
 +
            </div>
 +
           
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
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              ">
 +
              <a href="#buho" class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">STRUCTURE</a>
 +
            </div>
 +
           
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
 +
              ">
 +
              <a class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">ENTRY OF CONSUMABLES</a>
 +
            </div>
 +
           
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
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              ">
 +
              <a class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">REACTION ZONE</a>
 +
            </div>
 +
           
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
 +
              ">
 +
              <a class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">MEASUREMENT ZONE</a>
 +
            </div>
 +
           
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
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              line-height: 1.3em;
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              ">
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              <a class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">DISINFECTION AND STERILITY</a>
 +
            </div>
 +
           
 +
          </li>
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
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              ">
 +
              <a class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">SIMULTANEOUS EXPERIMENTS</a>
 +
            </div>
 +
           
 +
          </li>
 +
          </li>
 +
          <li class="lateral">
 +
            <div class="tab__title" style="
 +
              line-height: 1.3em;
 +
              ">
 +
              <a class="lateral" style="
 +
                color: #353535;
 +
                opacity: 1;
 +
              ">RESULTS</a>
 +
            </div>
 +
           
 +
          </li>
 +
        </ul><ul class="tabs-content"></ul>
 +
       
 +
       
 +
      </div>
 +
    </div>
  
  
 +
<!--------------------------------------------TEXTO------------------------------------------------>
  
<div class="column third_size">
 
<h3> What should this page contain?</h3>
 
  
<ul>
+
    <div class="col-md-9" style="
<li>Clearly state what the team accomplished</li>
+
      /* margin-left: 0px; */
<li>General Support</li>
+
      padding-left: 6em;
<li>Project support and advice</li>
+
      padding-right: 2em;
<li>Fundraising help and advice</li>
+
      ">
<li>Lab support</li>
+
      <div>
<li>Difficult technique support</li>
+
        <h3>HARDWARE</h3>
<li>Project advisor support</li>
+
        <h4>INTRODUCTION</h4>
<li>Wiki support</li>
+
<li>Presentation coaching</li>
+
<li>Human Practices support</li>
+
<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
+
</ul>
+
</div>
+
  
<div class="column third_size">
+
        <p>To address the lack of accessibility to synthetic biology due to the high costs and the level of knowledge required among others, our team has designed Printeria, a fully-equipped bioengineering device capable of automating the process of printing genetic circuits in bacteria.
<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
+
        </p>
<ul>
+
<li>Does your institution teach an iGEM or synthetic biology course?</li>
+
<li>When did you start this course?</li>
+
<li>Are the syllabus and course materials freely available online?</li>
+
<li>When did you start your brainstorming?</li>
+
<li>When did you start in the lab?</li>
+
<li>When did you start working on  your project?</li>
+
</ul>
+
</div>
+
  
 +
        <p> To ensure a functional, economical and easy-to-use design, collaboration between the hardware and the wet lab teams has been necessary throughout the development process. After several drafts we have achieved a unique design that can be easily transported and replicated at a cost not exceeding xxx € (xxx $). [and whose cost does not exceed xxx €]</p>
  
<div class="column third_size">
+
        <p> Printeria is divided into three main parts dependent on each other:</p>
<div class="highlight decoration_A_full">
+
  
<h3>Inspiration</h3>
+
        <div>
<p>Take a look at what other teams have done:</p>
+
        <ul>
<ul>
+
          <li>
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
+
            <p> <u>Entry of consumables</u>: components that allow the entry of consumables into the reaction zone.</p>
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
+
          </li>
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
+
          <li>
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
+
            <p> <u>Reaction zone</u>: space where the process of printing genetic circuits in bacteria occurs thanks to digital microfluidic technology.</p>
</ul>
+
          </li>
</div>
+
          <li>
</div>
+
            <p> <u>Measurement zone</u>: the bacteria are kept in conditions that allow for growth and expression while OD and fluorescence measurements are taken.</p>
 +
          </li>
 +
        </ul>
 +
      </div>
 +
<div>
 +
<table class="border--round table--alternate-row tableHec" style="width:100%;">
 +
          <thead><tr>
 +
            <th class="thHec">Part</th>
 +
            <th class="thHec">Quantity</th>
 +
            <th class="thHec">Cost per unit</th>
 +
            <th class="thHec">Cost</th>
 +
          </tr>
 +
        </thead><tbody><tr>
 +
        <td class="tdHec">Structure</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
      </tr>
 +
      <tr>
 +
        <td class="tdHec">Entry system</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
      </tr>
 +
      <tr>
 +
        <td class="tdHec">Reaction zone</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
      </tr>
 +
      <tr>
 +
        <td class="tdHec">Measurement zone</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
        <td class="tdHec">----</td>
 +
      </tr>
 +
      <tr>
 +
        <td class="tdHec" style="opacity: 0; border:none !important;"></td>
 +
        <td class="tdHec" style="opacity: 0; border:none !important;"></td>
 +
        <td class="tdHec" style="background-color: #909090; color: #fff">TOTAL</td>
 +
        <td class="tdHec">----</td>
 +
      </tr>
 +
    </tbody></table>
 +
  </div>
 +
        <h5>STRUCTURE</h5>
 +
        <h5 style="padding-left: 1em;">Frame</h5>
 +
        <div style="padding-left: 1em;">
 +
            <p>First of all, it is necessary to design a structure that confines all the elements necessary for the operation of the device inside it and also allows the creation of a sterile atmosphere to carry out the biological reactions.</p>
  
 +
            <p> In order to facilitate its transport, aluminum has been chosen for the frame, which in addition to its low weight, has other great qualities such as great resistance to corrosion and low cost.</p>
  
 +
            <p> It has been used 15x15x1mm square tube profiles joined by plastic corner connectors obtaining as a result a 415x360x280mm chassis. Thanks to the generated blueprints we have been able to contact a company specializing in cutting and handling aluminum that has provided us with all the parts as we required. As it was not possible for us to find plastic connectors that fit our needs, we have decided to design these parts ourselves and then print them on a 3D printer. </p>
  
<div class="clear extra_space"></div>
+
            <div>
 +
              <div class="row" style="
 +
                margin: 0;
 +
                ">
 +
                <div class="col-md-6" style="
 +
                  padding: 0;
 +
                  padding-right: 0.3em;
 +
                  ">
 +
                  <img src="https://via.placeholder.com/800x400">
 +
                </div>
 +
                <div class="col-md-6" style="
 +
                  padding: 0;
 +
                  padding-left: 0.3em;
 +
                  ">
 +
                  <img src="https://via.placeholder.com/800x400">
 +
                </div>
 +
                <div style="
 +
                  width: 100%;
 +
                  padding-left: 19em;
 +
                  padding-right: 1em;
 +
                  "><h6>Aluminum frame</h6>
 +
                </div>
 +
              </div>
 +
            </div>
 +
        </div>
  
<div class="column two_thirds_size">
+
            <h5 style="padding-left: 1em;">Methacrylate</h5>
<h3> Why is this page needed? </h3>
+
 
<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
+
            <div style="padding-left: 1em;">
<p>
+
              <p> To complete the structure, it is necessary to create walls that are attached to this frame and contribute to its stability. We have opted for the use of methacrylate due to its attractive appearance, durability and scratch resistance. </p>
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
+
 
 +
              <p> As well as the aluminum profiles, the blueprints created were used for ordering the custom-made parts to a company.</p>
 +
 
 +
              <div style="
 +
              padding-bottom: 1em;
 +
              text-align: right;
 +
              ">
 +
              <img src="https://via.placeholder.com/800x400" alt="" style="
 +
              margin-top: 2.8em;
 +
              margin-bottom: 0.8em;
 +
              border-radius: 0.3em;
 +
              ">
 +
              <div style="
 +
                width: 100%;
 +
                padding-left: 19em;
 +
                padding-right: 1em;
 +
                "><h6>Methacrylate cladding</h6>
 +
              </div>
 +
              </div>
 +
 
 +
              <p> Finally, 4mm diameter countersunk head screws DIN 7991 (M4 L25) and nuts DIN 934 (M4), both made of galvanized steel, were used for joining the methacrylate plates to the frame. </p>
 +
 
 +
            </div>
 +
 
 +
            <h5 style="padding-left: 1em;">Leveling system</h5>
 +
 
 +
            <div style="padding-left: 1em;">
 +
              <p> Given the great importance of keeping the reaction zone perfectly horizontal, it is necessary to use levelling feet. In addition, for checking the horizontal position, a bull’s eye level has been installed next to the reaction zone. </p>
 +
 
 +
                <div style="
 +
                  padding-bottom: 0.8em;
 +
                  text-align: right;
 +
                 
 +
                  ">
 +
                  <div class="row" style="margin: 0;">
 +
                      <div class="col-md-6" style="
 +
                        padding: 0;
 +
                        padding-right: 0.3em;
 +
                        ">
 +
                        <img src="https://via.placeholder.com/800x400" alt="" style="
 +
                        margin-top: 1.8em;
 +
                        margin-bottom: 0.8em;
 +
                        border-radius: 0.3em;
 +
                        ">
 +
                      </div>
 +
                      <div class="col-md-6" style="
 +
                        padding: 0;
 +
                        padding-left: 0.3em;
 +
                        ">
 +
                        <img src="https://via.placeholder.com/800x400" alt="" style="
 +
                        margin-top: 1.8em;
 +
                        margin-bottom: 0.8em;
 +
                        border-radius: 0.3em;
 +
                        ">
 +
                      </div>
 +
                      <div style="
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                        "><h6>Levelling system</h6>
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                  <thead><tr>
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                    <th class="thHec">Part</th>
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                    <th class="thHec">Quantity</th>
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                    <th class="thHec">Cost per unit</th>
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                    <th class="thHec">Cost</th>
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                  </tr>
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                </thead><tbody><tr>
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                <td class="tdHec">Aluminum profiles</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
              </tr>
 +
              <tr>
 +
                <td class="tdHec">Profiles' cutting and drilling</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
              </tr>
 +
              <tr>
 +
                <td class="tdHec">Custom-made methacrylate</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
              </tr>
 +
              <tr>
 +
                <td class="tdHec">Screws DIN 7991</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
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              </tr>
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              <tr>
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                <td class="tdHec">Nuts DIN 934</td>
 +
                <td class="tdHec">----</td>
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                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
              </tr>
 +
              <tr>
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                <td class="tdHec">Kg of PLA for the connectors</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
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              </tr>
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              <tr>
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                <td class="tdHec">Leeveling feet</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
                <td class="tdHec">----</td>
 +
              </tr>
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              <tr>
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                <td class="tdHec">Bull's eye level</td>
 +
                <td class="tdHec">----</td>
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                <td class="tdHec">----</td>
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                <td class="tdHec">----</td>
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              </tr>
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              <tr>
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                <td class="tdHec" style="opacity: 0; border:none !important;"></td>
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                <td class="tdHec" style="opacity: 0; border:none !important;"></td>
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                <td class="tdHec" style="background-color: #909090; color: #fff">TOTAL</td>
 +
                <td class="tdHec">----</td>
 +
              </tr>
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            </tbody></table> 
 +
 
 +
        </div>
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 +
        <h5>ENTRY OF CONSUMABLES</h5>
 +
 
 +
        </div>
 +
        <h5>REACTION ZONE</h5>
 +
 
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        <h5>MEASUREMENT ZONE</h5>
 +
 
 +
        <h5>DISINFECTION AND STERILITY</h5>
 +
 
 +
        <h5>SIMULTANEOUS EXPERIMENTS</h5>
 +
 
 +
        <h5>RESULTS</h5>
 +
 
 +
 
 +
 
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  </div>
 
</div>
 
</div>
  
<div class="column third_size">
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<div class="highlight decoration_B_full">
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</section>
<h3> Can we base our project on a previous one? </h3>
+
 
<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<!--------------------FIN TEXTO------------------------------>
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Revision as of 16:28, 14 September 2018

Stack Multipurpose HTML Template

HARDWARE

INTRODUCTION

To address the lack of accessibility to synthetic biology due to the high costs and the level of knowledge required among others, our team has designed Printeria, a fully-equipped bioengineering device capable of automating the process of printing genetic circuits in bacteria.

To ensure a functional, economical and easy-to-use design, collaboration between the hardware and the wet lab teams has been necessary throughout the development process. After several drafts we have achieved a unique design that can be easily transported and replicated at a cost not exceeding xxx € (xxx $). [and whose cost does not exceed xxx €]

Printeria is divided into three main parts dependent on each other:

  • Entry of consumables: components that allow the entry of consumables into the reaction zone.

  • Reaction zone: space where the process of printing genetic circuits in bacteria occurs thanks to digital microfluidic technology.

  • Measurement zone: the bacteria are kept in conditions that allow for growth and expression while OD and fluorescence measurements are taken.

Part Quantity Cost per unit Cost
Structure ---- ---- ----
Entry system ---- ---- ----
Reaction zone ---- ---- ----
Measurement zone ---- ---- ----
TOTAL ----
STRUCTURE
Frame

First of all, it is necessary to design a structure that confines all the elements necessary for the operation of the device inside it and also allows the creation of a sterile atmosphere to carry out the biological reactions.

In order to facilitate its transport, aluminum has been chosen for the frame, which in addition to its low weight, has other great qualities such as great resistance to corrosion and low cost.

It has been used 15x15x1mm square tube profiles joined by plastic corner connectors obtaining as a result a 415x360x280mm chassis. Thanks to the generated blueprints we have been able to contact a company specializing in cutting and handling aluminum that has provided us with all the parts as we required. As it was not possible for us to find plastic connectors that fit our needs, we have decided to design these parts ourselves and then print them on a 3D printer.

Aluminum frame
Methacrylate

To complete the structure, it is necessary to create walls that are attached to this frame and contribute to its stability. We have opted for the use of methacrylate due to its attractive appearance, durability and scratch resistance.

As well as the aluminum profiles, the blueprints created were used for ordering the custom-made parts to a company.

Methacrylate cladding

Finally, 4mm diameter countersunk head screws DIN 7991 (M4 L25) and nuts DIN 934 (M4), both made of galvanized steel, were used for joining the methacrylate plates to the frame.

Leveling system

Given the great importance of keeping the reaction zone perfectly horizontal, it is necessary to use levelling feet. In addition, for checking the horizontal position, a bull’s eye level has been installed next to the reaction zone.

Levelling system
Part Quantity Cost per unit Cost
Aluminum profiles ---- ---- ----
Profiles' cutting and drilling ---- ---- ----
Custom-made methacrylate ---- ---- ----
Screws DIN 7991 ---- ---- ----
Nuts DIN 934 ---- ---- ----
Kg of PLA for the connectors ---- ---- ----
Leeveling feet ---- ---- ----
Bull's eye level ---- ---- ----
TOTAL ----
ENTRY OF CONSUMABLES
REACTION ZONE
MEASUREMENT ZONE
DISINFECTION AND STERILITY
SIMULTANEOUS EXPERIMENTS
RESULTS