Difference between revisions of "Team:Cardiff Wales/Parts"

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<h1>Parts</h1>
 
<h1>Parts</h1>
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<p> Our team added several useful parts to the PhytoBrick registry, all of which are easy to use with either conventional iGEM cloning or using GoldenGate cloning. While we are happy with the parts we have created, we could only submit basic parts due to the iGEM plasmid backbone specifications, which present problems for submission of level 1 parts made with GoldenGate cloning. For this reason, all of our submitted parts are in the pSB1C3 plasmid, and are all individual parts, with the exception of one part, <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a>, which is a composite part ordered as a gBlock, so didn't need to be assembled using GoldenGate cloning. We were aware of this limitation starting our project, but thought its ease of use and flexibility outweighed the fact that we wouldn't be able to submit level 1 parts. Nevertheless, we have made and tested several composite parts, adding data to existing registry parts, and to our newer ones.</p>
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<p> Our team added several useful parts to the PhytoBrick registry, all of which are easy to use with either conventional iGEM cloning or using GoldenGate cloning. While we are happy with the parts we have created, we could only submit basic parts due to the iGEM plasmid backbone specifications, which present problems for submission of level 1 parts made with GoldenGate cloning. For this reason, all of our submitted parts are in the pSB1C3 plasmid, and are all individual parts, with the exception of one part, <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a composite part ordered as a gBlock, so didn't need to be assembled using GoldenGate cloning. We were aware of this limitation starting our project, but thought its ease of use and flexibility outweighed the fact that we wouldn't be able to submit level 1 parts. Nevertheless, we have made and tested several composite parts, adding data to existing registry parts, and to our newer ones.</p>
 
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Revision as of 11:05, 26 September 2018

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Parts

Our team added several useful parts to the PhytoBrick registry, all of which are easy to use with either conventional iGEM cloning or using GoldenGate cloning. While we are happy with the parts we have created, we could only submit basic parts due to the iGEM plasmid backbone specifications, which present problems for submission of level 1 parts made with GoldenGate cloning. For this reason, all of our submitted parts are in the pSB1C3 plasmid, and are all individual parts, with the exception of one part, BBa_K2810011, which is a composite part ordered as a gBlock, so didn't need to be assembled using GoldenGate cloning. We were aware of this limitation starting our project, but thought its ease of use and flexibility outweighed the fact that we wouldn't be able to submit level 1 parts. Nevertheless, we have made and tested several composite parts, adding data to existing registry parts, and to our newer ones.






Name Type Description Designer Length
BBa_BBa_K2810000 Regulatory WRKY intron for plant-targeted RNAi constructs Geraint Parry 295bp
BBa_BBa_K2810001 Reporter GUS reporter for plant expression Geraint Parry 1817bp
BBa_BBa_K2810002 Regulatory RTBV Vascular Specific Promotor Geraint Parry 759bp
BBa_BBa_K2810003 RNA Full sequence for Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_BBa_K2810004 RNA Complimentary sequence to Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_BBa_K2810005 RNA Coding sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_BBa_K2810006 RNA Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_BBa_K2810007 RNA siRNA from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_BBa_K2810008 RNA siRNA* from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_BBa_K2810009 Reporter mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible Ryan Coates 676bp
BBa_BBa_K2810010 Regulatory Regulatory region derived from the ribitol-responsive operon Ryan Coates 169bp
BBa_BBa_K2810011 Composite mCherry under control of the bacterial promoter and terminato Ryan Coates 858bp