Difference between revisions of "Team:Cardiff Wales/Parts"
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| − | <p> Our team added several useful parts to the PhytoBrick registry, all of which are | + | <p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite parts to the registry as they are held within the pGB-A2 Phytobrick only submit basic parts due to the iGEM plasmid backbone specifications, which present problems for submission of level 1 parts made with GoldenGate cloning. </P> |
| + | Therefore each of our submitted parts are in the pSB1C3 plasmid, and are all individual parts, with the exception of one part, <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a composite part ordered as a gBlock, so didn't need to be assembled using GoldenGate cloning. We were aware of this limitation starting our project, but thought its ease of use and flexibility outweighed the fact that we wouldn't be able to submit level 1 parts. Nevertheless, we have made and tested several composite parts, adding data to existing registry parts, and to our newer ones.</p> | ||
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Revision as of 20:26, 2 October 2018
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Parts
Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite parts to the registry as they are held within the pGB-A2 Phytobrick only submit basic parts due to the iGEM plasmid backbone specifications, which present problems for submission of level 1 parts made with GoldenGate cloning.
Therefore each of our submitted parts are in the pSB1C3 plasmid, and are all individual parts, with the exception of one part, BBa_K2810011, which is a composite part ordered as a gBlock, so didn't need to be assembled using GoldenGate cloning. We were aware of this limitation starting our project, but thought its ease of use and flexibility outweighed the fact that we wouldn't be able to submit level 1 parts. Nevertheless, we have made and tested several composite parts, adding data to existing registry parts, and to our newer ones.| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2810000 | Regulatory | WRKY intron for plant-targeted RNAi constructs | Geraint Parry | 295bp |
| BBa_K2810001 | Reporter | GUS reporter for plant expression | Geraint Parry | 1817bp |
| BBa_K2810002 | Regulatory | RTBV Vascular Specific Promotor | Geraint Parry | 759bp |
| BBa_K2810003 | RNA | Full sequence for Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
| BBa_K2810004 | RNA | Complimentary sequence to Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
| BBa_K2810005 | RNA | Coding sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
| BBa_K2810006 | RNA | Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
| BBa_K2810007 | RNA | siRNA from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
| BBa_K2810008 | RNA | siRNA* from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
| BBa_K2810009 | Reporter | mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible | Ryan Coates | 676bp |
| BBa_K2810010 | Regulatory | Regulatory region derived from the ribitol-responsive operon | Ryan Coates | 169bp |
| BBa_K2810011 | Composite | mCherry under control of the bacterial promoter and terminato | Ryan Coates | 858bp |