Difference between revisions of "Team:Valencia UPV/Improve"
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| − | <li><p><a href="http://parts.igem.org/Part:BBa_K2656004">BBa_K2656004</a>: the <a href="http://parts.igem.org/Part:BBa_J23106">J23106</a> promoter in its | + | <li><p><a href="http://parts.igem.org/Part:BBa_K2656004">BBa_K2656004</a>: the <a href="http://parts.igem.org/Part:BBa_J23106">J23106</a> promoter in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li> |
| − | <li><a href="http://parts.igem.org/Part:BBa_K2656009">BBa_K2656009</a>: the <a href="http://parts.igem.org/Part:BBa_B0030">B0030</a> ribosome biding site in its | + | <li><p><a href="http://parts.igem.org/Part:BBa_K2656009">BBa_K2656009</a>: the <a href="http://parts.igem.org/Part:BBa_B0030">B0030</a> ribosome biding site in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li> |
| − | <li><a href="http://parts.igem.org/Part:BBa_K2656020">BBa_K2656020</a>: The original part <a href=http://parts.igem.org/Part:BBa_K592101> BBa_K592101</a> compatible with the GoldenBraid | + | <li><p><a href="http://parts.igem.org/Part:BBa_K2656020">BBa_K2656020</a>: The original part <a href=http://parts.igem.org/Part:BBa_K592101> BBa_K592101</a> compatible with the GoldenBraid assembly method .</p></li> |
| − | <li><a href="http://parts.igem.org/Part:BBa_K2656026">BBa_K2656026</a>: the <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a> transcriptional terminator in its | + | <li><p><a href="http://parts.igem.org/Part:BBa_K2656026">BBa_K2656026</a>: the <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a> transcriptional terminator in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li> |
</ul> | </ul> | ||
Revision as of 09:42, 8 October 2018
Part Improvement
The BioBrick chosen to be improved was the YFP coding sequence: BBa_K592101. To do this, we first standardized the part to be compatible with the GoldenBraid 3.0 assembly method creating the part BBa_K2656021. Then, we created the part BBa_K2656020. This part is the CDS of the YFP with the addition of the degradation tag ssRA LVA.
To test the effect of the degradation tag, we designed an experiment with which we measured the increase in protein degradation due to this tag. To perform this experiment, we assembled two composite parts:
BBa_K2656004: the J23106 promoter in its GoldenBraid compatible version from our Part Collection
BBa_K2656009: the B0030 ribosome biding site in its GoldenBraid compatible version from our Part Collection
BBa_K2656020: The original part BBa_K592101 compatible with the GoldenBraid assembly method .
BBa_K2656026: the B0015 transcriptional terminator in its GoldenBraid compatible version from our Part Collection